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Protein

tRNA1(Val) (adenine(37)-N6)-methyltransferase

Gene

EbC_34210

Organism
Erwinia billingiae (strain Eb661)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the adenine in position 37 of tRNA1(Val) (anticodon cmo5UAC).UniRule annotationSAAS annotation

Catalytic activityi

S-adenosyl-L-methionine + adenine(37) in tRNA1(Val) = S-adenosyl-L-homocysteine + N(6)-methyladenine(37) in tRNA1(Val).UniRule annotationSAAS annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciEBIL634500:GHYX-3497-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA1(Val) (adenine(37)-N6)-methyltransferaseUniRule annotationSAAS annotation (EC:2.1.1.223UniRule annotationSAAS annotation)
Alternative name(s):
tRNA m6A37 methyltransferaseUniRule annotation
Gene namesi
Ordered Locus Names:EbC_34210Imported
OrganismiErwinia billingiae (strain Eb661)Imported
Taxonomic identifieri634500 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
ProteomesiUP000008793 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi634500.EbC_34210.

Structurei

3D structure databases

ProteinModelPortaliD8MVU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000283147.
KOiK15460.
OMAiDRLFCYH.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01872. tRNA_methyltr_YfiC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
IPR022882. tRNA_adenine-N6_MeTrfase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D8MVU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTIFICLCG LNDFSLRGYC PMLAPFPFQV SQMSQQKAVL RSNGFTFKQF
60 70 80 90 100
FVAHDRCAMK VGTDGVLLGA WAPVAGAQRV LDIGTGSGLI ALMLAQRTGQ
110 120 130 140 150
DVEVDAVELD EQAVGQAREN AAESPWADRI HVWQDDIVHW SKNAEQRYSL
160 170 180 190 200
IVSNPPYFVP GTDCASPERA SARYTTDLTH DVLLSCAEEL ITEEGYFCVV
210 220 230 240 250
LPASAGDKLV ELAQQRGWHL HCRTDISDTE MRPPNRVLLA LSPKPGEPFV
260 270
DRMTIRGPDH NYSEAHCSLT RDFYLFR
Length:277
Mass (Da):30,839
Last modified:October 5, 2010 - v1
Checksum:i41E535968A641244
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP236843 Genomic DNA. Translation: CAX60952.1.
RefSeqiWP_013203437.1. NC_014306.1.
YP_003742799.1. NC_014306.1.

Genome annotation databases

EnsemblBacteriaiCAX60952; CAX60952; EbC_34210.
KEGGiebi:EbC_34210.
PATRICi42309864. VBIErwBil95213_3758.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP236843 Genomic DNA. Translation: CAX60952.1.
RefSeqiWP_013203437.1. NC_014306.1.
YP_003742799.1. NC_014306.1.

3D structure databases

ProteinModelPortaliD8MVU5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi634500.EbC_34210.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX60952; CAX60952; EbC_34210.
KEGGiebi:EbC_34210.
PATRICi42309864. VBIErwBil95213_3758.

Phylogenomic databases

HOGENOMiHOG000283147.
KOiK15460.
OMAiDRLFCYH.

Enzyme and pathway databases

BioCyciEBIL634500:GHYX-3497-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01872. tRNA_methyltr_YfiC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
IPR022882. tRNA_adenine-N6_MeTrfase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae."
    Kube M., Migdoll A.M., Gehring I., Heitmann K., Mayer Y., Kuhl H., Knaust F., Geider K., Reinhardt R.
    BMC Genomics 11:393-393(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eb661Imported.

Entry informationi

Entry nameiD8MVU5_ERWBE
AccessioniPrimary (citable) accession number: D8MVU5
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: June 24, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.