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D8MKK6

- D8MKK6_ERWBE

UniProt

D8MKK6 - D8MKK6_ERWBE

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, EbC_01310
Organism
Erwinia billingiae (strain Eb661)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi104 – 1041Magnesium; catalytic By similarityUniRule annotation
Active sitei128 – 1281Proton acceptor By similarityUniRule annotation
Binding sitei155 – 1551Allosteric activator ADP By similarityUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei212 – 2121Allosteric activator ADP By similarityUniRule annotation
Binding sitei223 – 2231Substrate By similarityUniRule annotation
Binding sitei244 – 2441Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATP By similarityUniRule annotation
Nucleotide bindingi103 – 1064ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciEBIL634500:GHYX-135-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:EbC_01310Imported
OrganismiErwinia billingiae (strain Eb661)
Taxonomic identifieri634500 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeErwinia
ProteomesiUP000008793: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni126 – 1283Substrate binding By similarityUniRule annotation
Regioni170 – 1723Substrate binding By similarityUniRule annotation
Regioni186 – 1883Allosteric activator ADP binding By similarityUniRule annotation
Regioni214 – 2163Allosteric activator ADP binding By similarityUniRule annotation
Regioni250 – 2534Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D8MKK6-1 [UniParc]FASTAAdd to Basket

« Hide

MIKKIGVLTS GGDAPGMNAA IRGVVRAALS EGLEVCGVYD GYLGLYEDRM    50
ANLDRYSVSD MINRGGTFLG SARFPEFREE SVRTVCIENM KKRGIDALVV 100
IGGDGSYMGA KRLTEMGFPC IGLPGTIDND VAGTDYTIGY FTALETVVEA 150
IDRLRDTSSS HQRISIVEVM GRYCGDLTLA AAIAGGCEFI VLPEIPYTRE 200
ELVEEIKAGI AKGKKHAIVA ITEHICDIDE LAKYIEAETK RETRSTVLGH 250
IQRGGAPVAY DRILASRMGA YSIELLLQGY GGRCVGIQNE KMVHHDIIDA 300
IENMKRPFKG DWLDTAKKLY 320
Length:320
Mass (Da):34,942
Last modified:October 5, 2010 - v1
Checksum:i3E521D37B2901DC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FP236843 Genomic DNA. Translation: CAX57662.1.
RefSeqiWP_013200169.1. NC_014306.1.
YP_003739522.1. NC_014306.1.

Genome annotation databases

EnsemblBacteriaiCAX57662; CAX57662; EbC_01310.
GeneIDi9427768.
KEGGiebi:EbC_01310.
PATRICi42303018. VBIErwBil95213_0394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FP236843 Genomic DNA. Translation: CAX57662.1 .
RefSeqi WP_013200169.1. NC_014306.1.
YP_003739522.1. NC_014306.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAX57662 ; CAX57662 ; EbC_01310 .
GeneIDi 9427768.
KEGGi ebi:EbC_01310.
PATRICi 42303018. VBIErwBil95213_0394.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci EBIL634500:GHYX-135-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome comparison of the epiphytic bacteria Erwinia billingiae and E. tasmaniensis with the pear pathogen E. pyrifoliae."
    Kube M., Migdoll A.M., Gehring I., Heitmann K., Mayer Y., Kuhl H., Knaust F., Geider K., Reinhardt R.
    BMC Genomics 11:393-393(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Eb661Imported.

Entry informationi

Entry nameiD8MKK6_ERWBE
AccessioniPrimary (citable) accession number: D8MKK6
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: September 3, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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