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D8MDS4 (D8MDS4_LEUGT) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL CBL90992.1
Ordered Locus Names:LEGAS_0344 EMBL CBL90992.1
OrganismLeuconostoc gasicomitatum (strain DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10) [Complete proteome] [HAMAP] EMBL CBL90992.1
Taxonomic identifier762550 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLeuconostoc

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain140 – 349210ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding170 – 22556ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3031Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3161Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3161Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3181Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D8MDS4 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: C93BEB075744553F

FASTA37741,818
        10         20         30         40         50         60 
MTKKHVALLF GGNSSEHDVS KRSAQNFYDA IKATGKYEIT VFAIAQNGYF LDPESSNRVL 

        70         80         90        100        110        120 
ALEDEQPIVD AYMAKIDTTD PLARISALSE GGDFDIFFPV VHGNLGEDGT LQGLFKLLNK 

       130        140        150        160        170        180 
PYVGAPLRGH AVSFDKAMTK ELLTVNGIRN TKYVVLDPKT AKEWTWEKVV AALGDIVFVK 

       190        200        210        220        230        240 
AANQGSSVGI SRAKNAVEFE AALSDSFQYD YKVLVEQAVK GSRELEVGVI GNEEPIVSEI 

       250        260        270        280        290        300 
GAHTLPNQGS GDAWYDYNNK FVDNSDVLFE IPAKLPESVT NEVKDMAIQA YKVLNLRGEA 

       310        320        330        340        350        360 
RMDFLLDENN VPYLGEPNTL PGFTNMSLFK RLWDYSDIDN VKLVDMLITY GFAEFEKNAQ 

       370 
LSYQFVTLGE EKIGKFN 

« Hide

References

[1]"Genome sequence and comparative genomics of a food spoilage lactic acid bacterium Leuconostoc gasicomitatum 18811T."
Johansson P., Paulin L., Vihavainen E.J., Salovuori N., Alatalo E.R., Bjoerkroth J.K., Auvinen P.
Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FN822744 Genomic DNA. Translation: CBL90992.1.
RefSeqYP_003771811.1. NC_014319.1.

3D structure databases

ProteinModelPortalD8MDS4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBL90992; CBL90992; LEGAS_0344.
GeneID9398237.
KEGGlgs:LEGAS_0344.
PATRIC42382332. VBILeuGas160647_0352.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.

Enzyme and pathway databases

BioCycLGAS762550:GHH1-1182-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD8MDS4_LEUGT
AccessionPrimary (citable) accession number: D8MDS4
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: February 19, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)