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D8MDJ3

- D8MDJ3_LEUGT

UniProt

D8MDJ3 - D8MDJ3_LEUGT

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Protein
3-isopropylmalate dehydratase large subunit
Gene
leuC, LEGAS_0263
Organism
Leuconostoc gasicomitatum (strain DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity.UniRule annotationSAAS annotations

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotationSAAS annotations

Cofactori

Binds 1 4Fe-4S cluster per subunit By similarity.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi339 – 3391Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi399 – 3991Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi402 – 4021Iron-sulfur (4Fe-4S) By similarityUniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotations

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotations, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciLGAS762550:GHH1-1424-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomerase
Isopropylmalate isomerase
Gene namesi
Name:leuCUniRule annotationImported
Ordered Locus Names:LEGAS_0263Imported
OrganismiLeuconostoc gasicomitatum (strain DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10)Imported
Taxonomic identifieri762550 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
ProteomesiUP000008706: Chromosome

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliD8MDJ3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000226972.
KOiK01703.
OMAiTAREESK.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D8MDJ3-1 [UniParc]FASTAAdd to Basket

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MSKTLFDKIW EKHVITGEIG EAQLIYVDLH LIHEVTSPQP FDGLRNTNRR    50
VRRPDLTFAT MDHNVSTKDI FNVQDHMSRL QMDTLVKNTK EFGVPLASIG 100
DDKQGIVHVV GPERGLTQPA KLIVCGDSHT ATHGAFGAIA FGIGTSEVEH 150
VLATQTIWQV KPKTMGIKVT GKLLKNTYAK DIIMGIIAKY GVSFGVGYAI 200
EFYGETVENL SMEARMTMCN MSIEAGSRTG MVQPDQTTFD YIEGREQAPK 250
DFEAAKNYWL QFYTDDESDF DETLTFDVSN LKPMVTWGTN PGMATPVDQS 300
LPAIKDDNDA NANAYEYIGL HPHMKATDIN LDYIFIGSCT NSRYEDLEIA 350
ANMMKGHHLA PNVTAWIVPG SRAIRNRAIK SGIAKIFEDA GCEWREPGCS 400
ACLAMNPDKI PAGKHVASTS NRNFIGRQGA GSRTHLASPA MVAAAGIAGY 450
FVDITVDEFV 460
Length:460
Mass (Da):50,606
Last modified:October 5, 2010 - v1
Checksum:i123845047F32D05E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN822744 Genomic DNA. Translation: CBL90911.1.
RefSeqiYP_003771730.1. NC_014319.1.

Genome annotation databases

EnsemblBacteriaiCBL90911; CBL90911; LEGAS_0263.
GeneIDi9399465.
KEGGilgs:LEGAS_0263.
PATRICi42382166. VBILeuGas160647_0271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN822744 Genomic DNA. Translation: CBL90911.1 .
RefSeqi YP_003771730.1. NC_014319.1.

3D structure databases

ProteinModelPortali D8MDJ3.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBL90911 ; CBL90911 ; LEGAS_0263 .
GeneIDi 9399465.
KEGGi lgs:LEGAS_0263.
PATRICi 42382166. VBILeuGas160647_0271.

Phylogenomic databases

HOGENOMi HOG000226972.
KOi K01703.
OMAi TAREESK.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci LGAS762550:GHH1-1424-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence and comparative genomics of a food spoilage lactic acid bacterium Leuconostoc gasicomitatum 18811T."
    Johansson P., Paulin L., Vihavainen E.J., Salovuori N., Alatalo E.R., Bjoerkroth J.K., Auvinen P.
    Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10.

Entry informationi

Entry nameiD8MDJ3_LEUGT
AccessioniPrimary (citable) accession number: D8MDJ3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: September 3, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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