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D8MDJ3

- D8MDJ3_LEUGT

UniProt

D8MDJ3 - D8MDJ3_LEUGT

Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Leuconostoc gasicomitatum (strain DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotationSAAS annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi339 – 3391Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi399 – 3991Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi402 – 4021Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LyaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciLGAS762550:GHH1-1424-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
    Alternative name(s):
    Alpha-IPM isomeraseUniRule annotation
    Isopropylmalate isomeraseUniRule annotation
    Gene namesi
    Name:leuCUniRule annotationImported
    Ordered Locus Names:LEGAS_0263Imported
    OrganismiLeuconostoc gasicomitatum (strain DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10)Imported
    Taxonomic identifieri762550 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
    ProteomesiUP000008706: Chromosome

    Interactioni

    Subunit structurei

    Heterodimer of LeuC and LeuD.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD8MDJ3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000226972.
    KOiK01703.
    OMAiTAREESK.

    Family and domain databases

    Gene3Di3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPiMF_01026. LeuC_type1.
    InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR00170. leuC. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D8MDJ3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKTLFDKIW EKHVITGEIG EAQLIYVDLH LIHEVTSPQP FDGLRNTNRR    50
    VRRPDLTFAT MDHNVSTKDI FNVQDHMSRL QMDTLVKNTK EFGVPLASIG 100
    DDKQGIVHVV GPERGLTQPA KLIVCGDSHT ATHGAFGAIA FGIGTSEVEH 150
    VLATQTIWQV KPKTMGIKVT GKLLKNTYAK DIIMGIIAKY GVSFGVGYAI 200
    EFYGETVENL SMEARMTMCN MSIEAGSRTG MVQPDQTTFD YIEGREQAPK 250
    DFEAAKNYWL QFYTDDESDF DETLTFDVSN LKPMVTWGTN PGMATPVDQS 300
    LPAIKDDNDA NANAYEYIGL HPHMKATDIN LDYIFIGSCT NSRYEDLEIA 350
    ANMMKGHHLA PNVTAWIVPG SRAIRNRAIK SGIAKIFEDA GCEWREPGCS 400
    ACLAMNPDKI PAGKHVASTS NRNFIGRQGA GSRTHLASPA MVAAAGIAGY 450
    FVDITVDEFV 460
    Length:460
    Mass (Da):50,606
    Last modified:October 5, 2010 - v1
    Checksum:i123845047F32D05E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN822744 Genomic DNA. Translation: CBL90911.1.
    RefSeqiYP_003771730.1. NC_014319.1.

    Genome annotation databases

    EnsemblBacteriaiCBL90911; CBL90911; LEGAS_0263.
    GeneIDi9399465.
    KEGGilgs:LEGAS_0263.
    PATRICi42382166. VBILeuGas160647_0271.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN822744 Genomic DNA. Translation: CBL90911.1 .
    RefSeqi YP_003771730.1. NC_014319.1.

    3D structure databases

    ProteinModelPortali D8MDJ3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBL90911 ; CBL90911 ; LEGAS_0263 .
    GeneIDi 9399465.
    KEGGi lgs:LEGAS_0263.
    PATRICi 42382166. VBILeuGas160647_0271.

    Phylogenomic databases

    HOGENOMi HOG000226972.
    KOi K01703.
    OMAi TAREESK.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci LGAS762550:GHH1-1424-MONOMER.

    Family and domain databases

    Gene3Di 3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPi MF_01026. LeuC_type1.
    InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR00170. leuC. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence and comparative genomics of a food spoilage lactic acid bacterium Leuconostoc gasicomitatum 18811T."
      Johansson P., Paulin L., Vihavainen E.J., Salovuori N., Alatalo E.R., Bjoerkroth J.K., Auvinen P.
      Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10Imported.

    Entry informationi

    Entry nameiD8MDJ3_LEUGT
    AccessioniPrimary (citable) accession number: D8MDJ3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3