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D8MDJ3

- D8MDJ3_LEUGT

UniProt

D8MDJ3 - D8MDJ3_LEUGT

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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Leuconostoc gasicomitatum (strain DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotationSAAS annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotationSAAS annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi339 – 3391Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi399 – 3991Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi402 – 4021Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotation

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciLGAS762550:GHH1-1424-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotationImported
Ordered Locus Names:LEGAS_0263Imported
OrganismiLeuconostoc gasicomitatum (strain DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10)Imported
Taxonomic identifieri762550 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc
ProteomesiUP000008706: Chromosome

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD8MDJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226972.
KOiK01703.
OMAiTAREESK.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D8MDJ3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKTLFDKIW EKHVITGEIG EAQLIYVDLH LIHEVTSPQP FDGLRNTNRR
60 70 80 90 100
VRRPDLTFAT MDHNVSTKDI FNVQDHMSRL QMDTLVKNTK EFGVPLASIG
110 120 130 140 150
DDKQGIVHVV GPERGLTQPA KLIVCGDSHT ATHGAFGAIA FGIGTSEVEH
160 170 180 190 200
VLATQTIWQV KPKTMGIKVT GKLLKNTYAK DIIMGIIAKY GVSFGVGYAI
210 220 230 240 250
EFYGETVENL SMEARMTMCN MSIEAGSRTG MVQPDQTTFD YIEGREQAPK
260 270 280 290 300
DFEAAKNYWL QFYTDDESDF DETLTFDVSN LKPMVTWGTN PGMATPVDQS
310 320 330 340 350
LPAIKDDNDA NANAYEYIGL HPHMKATDIN LDYIFIGSCT NSRYEDLEIA
360 370 380 390 400
ANMMKGHHLA PNVTAWIVPG SRAIRNRAIK SGIAKIFEDA GCEWREPGCS
410 420 430 440 450
ACLAMNPDKI PAGKHVASTS NRNFIGRQGA GSRTHLASPA MVAAAGIAGY
460
FVDITVDEFV
Length:460
Mass (Da):50,606
Last modified:October 5, 2010 - v1
Checksum:i123845047F32D05E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN822744 Genomic DNA. Translation: CBL90911.1.
RefSeqiYP_003771730.1. NC_014319.1.

Genome annotation databases

EnsemblBacteriaiCBL90911; CBL90911; LEGAS_0263.
GeneIDi9399465.
KEGGilgs:LEGAS_0263.
PATRICi42382166. VBILeuGas160647_0271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN822744 Genomic DNA. Translation: CBL90911.1 .
RefSeqi YP_003771730.1. NC_014319.1.

3D structure databases

ProteinModelPortali D8MDJ3.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBL90911 ; CBL90911 ; LEGAS_0263 .
GeneIDi 9399465.
KEGGi lgs:LEGAS_0263.
PATRICi 42382166. VBILeuGas160647_0271.

Phylogenomic databases

HOGENOMi HOG000226972.
KOi K01703.
OMAi TAREESK.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci LGAS762550:GHH1-1424-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence and comparative genomics of a food spoilage lactic acid bacterium Leuconostoc gasicomitatum 18811T."
    Johansson P., Paulin L., Vihavainen E.J., Salovuori N., Alatalo E.R., Bjoerkroth J.K., Auvinen P.
    Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15947 / CECT 5767 / JCM 12535 / LMG 18811 / TB1-10Imported.

Entry informationi

Entry nameiD8MDJ3_LEUGT
AccessioniPrimary (citable) accession number: D8MDJ3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: October 1, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3