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Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Nitrosococcus watsoni (strain C-113)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei131Substrate; in homodimeric partnerUniRule annotation1
Binding sitei181SubstrateUniRule annotation1
Active sitei183Proton acceptorUniRule annotation1
Binding sitei185SubstrateUniRule annotation1
Metal bindingi209Magnesium; via carbamate groupUniRule annotation1
Metal bindingi211MagnesiumUniRule annotation1
Metal bindingi212MagnesiumUniRule annotation1
Active sitei301Proton acceptorUniRule annotation1
Binding sitei302SubstrateUniRule annotation1
Binding sitei334SubstrateUniRule annotation1
Sitei341Transition state stabilizerUniRule annotation1
Binding sitei386SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Calvin cycleUniRule annotation, Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotation
Ordered Locus Names:Nwat_2772Imported
OrganismiNitrosococcus watsoni (strain C-113)Imported
Taxonomic identifieri105559 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus
Proteomesi
  • UP000000393 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei209N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Protein-protein interaction databases

STRINGi105559.Nwat_2772.

Structurei

3D structure databases

ProteinModelPortaliD8KAW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 152RuBisCO_large_NInterPro annotationAdd BLAST127
Domaini162 – 469RuBisCO_largeInterPro annotationAdd BLAST308

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DT1. Bacteria.
COG1850. LUCA.
KOiK01601.
OMAiEAQYFAY.
OrthoDBiPOG091H14UZ.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D8KAW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSETMAEG KERYQSGVIP YKKMGYWEPD YQPKETDIIA MFRITPQPGV
60 70 80 90 100
DPEEAAAAVA GESSTATWTV VWTDRLTACE LYRAKAYRID PVPNTGEGTK
110 120 130 140 150
NEAQYFAYIA YDLDLFEPGS IANLTASIIG NVFGFKAVKA LRLEDMRIPV
160 170 180 190 200
AYLKTFQGPA TGVVVERERL DKFGRPLLGA TTKPKLGLSG RNYGRVVYEA
210 220 230 240 250
LKGGLDFVKD DENINSQPFM HWRDRFLYCM EAVNKASAAT GEVKGHYLNV
260 270 280 290 300
TAATMEDMYE RAEFAKSLGS VIIMIDLVVG YTAIQSMAKW ARKNDMILHL
310 320 330 340 350
HRAGNSTYSR QKNHGMNFRV ICKWMRMAGV DHIHAGTVVG KLEGDPLMIK
360 370 380 390 400
GFYDTLLDSH TPTSLEHGLF FDQDWASLNK VMPVASGGIH AGQMHQLIQY
410 420 430 440 450
LGEDVILQFG GGTIGHPQGI QAGAVANRVA LEAMILARNE GRDYVKEGPQ
460 470 480 490
ILQDAAKWCS PLKAALDTWK DVTFNYESTD TADFVPTATA SV
Length:492
Mass (Da):54,425
Last modified:October 5, 2010 - v1
Checksum:i2ED50814934759BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002086 Genomic DNA. Translation: ADJ29538.1.
RefSeqiWP_013221603.1. NC_014315.1.

Genome annotation databases

EnsemblBacteriaiADJ29538; ADJ29538; Nwat_2772.
KEGGinwa:Nwat_2772.
PATRICi42238239. VBINitWat28885_2971.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002086 Genomic DNA. Translation: ADJ29538.1.
RefSeqiWP_013221603.1. NC_014315.1.

3D structure databases

ProteinModelPortaliD8KAW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi105559.Nwat_2772.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADJ29538; ADJ29538; Nwat_2772.
KEGGinwa:Nwat_2772.
PATRICi42238239. VBINitWat28885_2971.

Phylogenomic databases

eggNOGiENOG4105DT1. Bacteria.
COG1850. LUCA.
KOiK01601.
OMAiEAQYFAY.
OrthoDBiPOG091H14UZ.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiD8KAW1_NITWC
AccessioniPrimary (citable) accession number: D8KAW1
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: November 30, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.