Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

D8K8Z0 (D8K8Z0_NITWC) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Nwat_0230 EMBL ADJ27200.1
OrganismNitrosococcus watsoni (strain C-113) [Complete proteome] [HAMAP] EMBL ADJ27200.1
Taxonomic identifier105559 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesChromatiaceaeNitrosococcus

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain109 – 304196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding135 – 19056ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2581Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2711Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2711Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2731Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D8K8Z0 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: FB5406A8D7355FA4

FASTA31634,257
        10         20         30         40         50         60 
MIGRADAKAW GKVAVLMGGY SAEREISLKS GTAVLQALLR QGIEAHGIDV GKEVLTQLSK 

        70         80         90        100        110        120 
GEFTRAFIVL HGRGGEDGVI QGALETLNLP YTGSGVLGSA LTMDKLRSKQ LWRGMDLPTA 

       130        140        150        160        170        180 
DFSALTEDTD PAVIAADLGL PLIVKPAREG SSLGMMKVES TDALQSAYRE AAIFDTAVFA 

       190        200        210        220        230        240 
ERWLSGAEYT AAILADRVLP LIRLETPRVF YDFEAKYHAN TTHYFCPCGL SERQEHVLQA 

       250        260        270        280        290        300 
LALEAFQVLG ASGWGRVDLR CDEKAHPYLL EINTVPGMTD HSLVPMAAQA AGIGFDEMVL 

       310 
RILESSLERR MFQDGA 

« Hide

References

[1]"Complete sequence of chromosome of Nitrosococcus watsoni C-113."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Malfatti S.A., Chain P.S.G., Land M., Hauser L., Kyrpides N., Ivanova N., Cambell M.A., Heidelberg J.F., Klotz M.G., Woyke T.
Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C-113 EMBL ADJ27200.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002086 Genomic DNA. Translation: ADJ27200.1.
RefSeqYP_003759521.1. NC_014315.1.

3D structure databases

ProteinModelPortalD8K8Z0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADJ27200; ADJ27200; Nwat_0230.
GeneID9391682.
KEGGnwa:Nwat_0230.
PATRIC42232719. VBINitWat28885_0251.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMADTQYRIP.

Enzyme and pathway databases

BioCycNWAT105559:GHXU-235-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD8K8Z0_NITWC
AccessionPrimary (citable) accession number: D8K8Z0
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: February 19, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)