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D8IH31 (D8IH31_LACFC) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:LC40_0318 EMBL ADJ40962.1
OrganismLactobacillus fermentum (strain CECT 5716) [Complete proteome] [HAMAP] EMBL ADJ40962.1
Taxonomic identifier712938 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain141 – 347207ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding171 – 22656ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3011Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3161Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D8IH31 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: 036A4E0283EBDD07

FASTA37742,319
        10         20         30         40         50         60 
MTEKKLHVAL LFGGNSSEHD VSKRSAHNIY DGMDKDKYDV SIFMFTKDGI LLDNEASQRI 

        70         80         90        100        110        120 
FDGEPEDQVV QEAYQKMDLD APLAPIAALS TVKEIDFFYP VIHGNLGEDG TVQGLFRLLK 

       130        140        150        160        170        180 
KPYIGSGIAS SAMSFDKDLT KRILNQAGIR NTKYLLVTPQ NKDQYSWSRI KEELGDLVFV 

       190        200        210        220        230        240 
KPAKQGSSVG IHKVDTEEEY ETAMKDAFTY DYKVLVEAGI KNPREIEISI LGNENPIASK 

       250        260        270        280        290        300 
LGGIRVPEGD EFYDYENKFV DASGVVFDLP VIVDDDLAKE ITDMALKAYE ALGMKGMARI 

       310        320        330        340        350        360 
DFLVDEDGVP YLGEPNTLPG FTNISLYPQM WNISGISYSE LIDRLIQLGI EEFEHEGQLR 

       370 
YDFKALGVEK VGEKRYN 

« Hide

References

[1]"Complete genome sequence of Lactobacillus fermentum CECT 5716, a probiotic strain isolated from human milk."
Jimenez E., Langa S., Martin V., Arroyo R., Martin R., Fernandez L., Rodriguez J.M.
J. Bacteriol. 192:4800-4800(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CECT 5716 EMBL ADJ40962.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002033 Genomic DNA. Translation: ADJ40962.1.
RefSeqYP_005848456.1. NC_017465.1.

3D structure databases

ProteinModelPortalD8IH31.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADJ40962; ADJ40962; LC40_0318.
GeneID12456310.
KEGGlfr:LC40_0318.
PATRIC43084925. VBILacFer149467_0447.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.

Enzyme and pathway databases

BioCycLFER712938:GLF8-312-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD8IH31_LACFC
AccessionPrimary (citable) accession number: D8IH31
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)