D8I6T0 (D8I6T0_AMYMU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Urocanate hydratase HAMAP-Rule MF_00577
Short name=Urocanase HAMAP-Rule MF_00577
EC=4.2.1.49 HAMAP-Rule MF_00577

Alternative name(s):
Imidazolonepropionate hydrolase HAMAP-Rule MF_00577

Gene names

Name:	hutU HAMAP-Rule MF_00577 EMBL ADJ43080.1
Ordered Locus Names:	AMED_1266

Organism

Amycolatopsis mediterranei (strain U-32) [Complete proteome] [HAMAP] EMBL ADJ43080.1

Taxonomic identifier

749927 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis › Amycolatopsis mediterranei

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O. HAMAP-Rule MF_00577 SAAS SAAS023637
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP-Rule MF_00577 SAAS SAAS023636
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3. HAMAP-Rule MF_00577 SAAS SAAS023637
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00577.
Sequence similarities	Belongs to the urocanase family. HAMAP-Rule MF_00577

Ontologies

Keywords
Biological process	Histidine metabolism HAMAP-Rule MF_00577 SAAS SAAS023637
Cellular component	Cytoplasm HAMAP-Rule MF_00577
Ligand	NAD HAMAP-Rule MF_00577 SAAS SAAS023637
Molecular function	Lyase HAMAP-Rule MF_00577 SAAS SAAS023637
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: UniProtKB-UniPathway histidine catabolic process to glutamate and formate Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	urocanate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	403	1	By similarity HAMAP-Rule MF_00577

Sequences

	Sequence		Length	Mass (Da)
	D8I6T0 [UniParc]. Last modified October 5, 2010. Version 1. Checksum: 2D0B372B096724D1	FASTA	550	59,680
	10 20 30 40 50 60 MSRVVRAARG TQLTAKSWQT EAALRMFHNN LDPDVAERPE DLVVYGGTGK AARNWASFDA 70 80 90 100 110 120 ITRELTTLDV DETLLVQSGK PVGVFRTHEW APRVLIANSN LVGDWATWPE FRRLEQQGLT 130 140 150 160 170 180 MYGQMTAGSW IYIGTQGILQ GTYETFAAVA KKKFGGSLRG TLTVTAGLGG MGGAQPLAVT 190 200 210 220 230 240 MNDGVAMVIE CDPQRAHRRV ETRYLDEVAD DLDDAIARVT KAKQEKRPLS VGVVGNAAEV 250 260 270 280 290 300 LPELLRRGVE VDIVTDQTSA HDPLSYLPKG VSVDDWHDYA AKKPDEFTDR SRESMADHVD 310 320 330 340 350 360 AMLGFLDRGA EVFDYGNSLR GEAKLGGCER AFDFPGFVPA YIRPLFCEGN GPFRWAALSG 370 380 390 400 410 420 DPEDIAATDR AMLELFPENE SLARWIKLAG ERVAFQGLPA RICWLGYGER HLAGLRFNEM 430 440 450 460 470 480 VASGELKAPV VIGRDHLDSG SVASPYRETE GMADGSDAIA DWPLLNALVN TSSGASWVSI 490 500 510 520 530 540 HHGGGVGMGR SIHAGQVSVA DGTLLAAQKL ERVLTNDPGM GVIRHVDAGY DQAADVADER 550 GVRVPMREDA

References

[1]

"Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism." Zhao W., Zhong Y., Yuan H., Wang J., Zheng H., Wang Y., Cen X., Xu F., Bai J., Han X., Lu G., Zhu Y., Shao Z., Yan H., Li C., Peng N., Zhang Z., Zhang Y. , Lin W., Fan Y., Qin Z., Hu Y., Zhu B., Wang S., Ding X., Zhao G.P. Cell Res. 20:1096-1108(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: U-32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002000 Genomic DNA. Translation: ADJ43080.1.
RefSeq	YP_003763482.1. NC_014318.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADJ43080; ADJ43080; AMED_1266.
GeneID	9435483.
KEGG	amd:AMED_1266.
PATRIC	42149495. VBIAmyMed151214_1302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000237606.
KO	K01712.
Enzyme and pathway databases
BioCyc	AMED749927:GCZN-1274-MONOMER.
UniPathway	UPA00379; UER00550.
Family and domain databases
HAMAP	MF_00577. HutU.
InterPro	IPR023637. Urocanase. IPR023636. Urocanase_CS. [Graphical view]
PANTHER	PTHR32312. PTHR32312. 1 hit.
Pfam	PF01175. Urocanase. 1 hit. [Graphical view]
PIRSF	PIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAM	SSF111326. Urocanase. 1 hit.
TIGRFAMs	TIGR01228. hutU. 1 hit.
PROSITE	PS01233. UROCANASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D8I6T0_AMYMU

Accession

Primary (citable) accession number: D8I6T0

Entry history

Integrated into UniProtKB/TrEMBL:	October 5, 2010
Last sequence update:	October 5, 2010
Last modified:	May 29, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)