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D8GBI5 (D8GBI5_LACCZ) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:LCAZH_0156 EMBL ADK17452.1
OrganismLactobacillus casei (strain Zhang) [Complete proteome] [HAMAP] EMBL ADK17452.1
Taxonomic identifier498216 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain145 – 339195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding172 – 22554ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2941Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D8GBI5 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: EE1EF6A679CAA405

FASTA35739,020
        10         20         30         40         50         60 
MKIVVLAGGR STERNVSISS GYRITNALRQ KGQQATFIDL FLGYDLEGKT VEQVFDDANT 

        70         80         90        100        110        120 
SKDLNISDAI LTDEDINKLR PDGSTQLFGP NVMAICKAAD IVFLALHGGD GENGKVQAVF 

       130        140        150        160        170        180 
DINGVKYTGS GPLASGITMN KVFSKEVMLY HGIQTSGFKE FKRDQGPKQT VPFDFPVVVK 

       190        200        210        220        230        240 
PTSGGSSVGT HIIHNQEELE SGLEDVFRFD NSAIVEEFTP GREFSLGVVN GHAYSAIEIK 

       250        260        270        280        290        300 
VRSGWYDFKH KFQAGYTDFI TPPKDLDEDV HQAMKDVAVQ TMDVLGLQNY GRIDFFANEK 

       310        320        330        340        350 
GVWVIEANNL PGMTPLSLLP QEAEADGVDY GDLVMDIVNG KLKLYADGMT EAGLLTK 

« Hide

References

« Hide 'large scale' references
[1]Meng H.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Zhang.
[2]"Complete genome sequence of Lactobacillus casei Zhang, a new probiotic strain isolated from traditional homemade koumiss in Inner Mongolia, China."
Zhang W., Yu D., Sun Z., Wu R., Chen X., Chen W., Meng H., Hu S., Zhang H.
J. Bacteriol. 192:5268-5269(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Zhang EMBL ADK17452.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001084 Genomic DNA. Translation: ADK17452.1.
RefSeqYP_003787302.1. NC_014334.1.

3D structure databases

ProteinModelPortalD8GBI5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADK17452; ADK17452; LCAZH_0156.
GeneID9458076.
KEGGlcz:LCAZH_0156.
PATRIC42376239. VBILacCas45142_0145.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.

Enzyme and pathway databases

BioCycLCAS498216:GH2S-156-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD8GBI5_LACCZ
AccessionPrimary (citable) accession number: D8GBI5
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)