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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Lactobacillus plantarum subsp. plantarum ATCC 14917 = JCM 1149 = CGMCC 1.2437
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Mn2+SAAS annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase (glutamine-hydrolyzing) (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171
UPA00070; UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotationImported
ORF Names:HMPREF0531_12320Imported
OrganismiLactobacillus plantarum subsp. plantarum ATCC 14917 = JCM 1149 = CGMCC 1.2437Imported
Taxonomic identifieri525338 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000005567 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD7VDU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 401Carboxyphosphate synthetic domainUniRule annotationAdd BLAST401
Regioni545 – 927Carbamoyl phosphate synthetic domainUniRule annotationAdd BLAST383
Regioni928 – 1020Allosteric domainUniRule annotationAdd BLAST93

Sequence similaritiesi

Belongs to the CarB family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotation

Phylogenomic databases

OrthoDBiPOG091H01IP

Family and domain databases

Gene3Di1.10.1030.10, 1 hit
3.30.1490.20, 1 hit
HAMAPiMF_01210_B CPSase_L_chain_B, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006275 CarbamoylP_synth_lsu
IPR005480 CarbamoylP_synth_lsu_oligo
IPR036897 CarbamoylP_synth_lsu_oligo_sf
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR005483 CbamoylP_synth_lsu_CPSase_dom
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF02786 CPSase_L_D2, 2 hits
PF02787 CPSase_L_D3, 1 hit
PRINTSiPR00098 CPSASE
SMARTiView protein in SMART
SM01096 CPSase_L_D3, 1 hit
SUPFAMiSSF48108 SSF48108, 1 hit
SSF52440 SSF52440, 2 hits
TIGRFAMsiTIGR01369 CPSaseII_lrg, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 2 hits
PS00866 CPSASE_1, 2 hits
PS00867 CPSASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

D7VDU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKNNAIHSI AVIGSGPIKI GQAAEFDYAG TQACLSLKAA GYHVILINSN
60 70 80 90 100
PATIMTDTAT ADEVYLEPLT LTSVTKILRA THPDALLPTL GGQTGLNLAM
110 120 130 140 150
ALDQAGVLNE LKIQLLGTSL ATINQAEDRA AFKTLMKRLH QPIPASTTVH
160 170 180 190 200
HVTSALSFAE KIGYPVIVRP AFTLGGSGGG IANNAAELTQ TLQRGLTMSP
210 220 230 240 250
VTECLIEQSI AGFKEIEFEV MRDNQGTKII VCSMENFDPV GIHTGDSIVY
260 270 280 290 300
APVQTLTDTE YQQLRTATLT IVEALDIRGG CNVQLAQDPN SRQYYVIEVN
310 320 330 340 350
PRVSRSSALA SKATGYPIAK IAADIAIGLN LSEINNPVTQ TTYAAFEPAL
360 370 380 390 400
DYVVAKIPRF AFDKFPTADA HLGTQMKATG EVMAIGSTLE EATLKAIASL
410 420 430 440 450
EIDPKIQASL TPDHHVTTTE YIDQLTHPTD QRLFYLLAAL QAGWPLAKLA
460 470 480 490 500
TLTQITPFFL SKLQHIAQLI RNIKQVPTSQ HLLSAKKYGV SLATMAHYAQ
510 520 530 540 550
TSVATIAAMT ADLPFVYKMV DTCAGEFASV TPYFYSTAFG QTNESHPLGH
560 570 580 590 600
SILVLGSGPI RIGQGIEFDY TTVHCVKAIQ QAGYHAIIVN NNPETVSTDF
610 620 630 640 650
STSDKLYFEP LTIERLMPII ALEQPAGVIV QFGGQTAINL AHQLTKLGVT
660 670 680 690 700
VLGTSVSATD LTEDRQSFAD CLRLLKIAQA AGTTVTELSG ARQAAHAIGY
710 720 730 740 750
PLLVRPSFVL GGRAMAIVHN DHELTPVIKS AVAAGHGAPI LMDQYLAGTE
760 770 780 790 800
CEVDVLSDGT SCFIPGIMEH IEGAGVHSGD SITVYPPQHL SPAVQDKIVT
810 820 830 840 850
IATKLAQHLH CVGMMNIQFV VTDDVYVIDV NPRASRTVPY MSKVTHLPLA
860 870 880 890 900
QLATRLILGQ SLATLGLVPG LLTPVPQQIA IKAPVFSFNK LPQSPVVLSP
910 920 930 940 950
EMKSTGETLG IGPTFTTAWH AAMADSYHLE TWQTVDGLIT DIATVAQPAV
960 970 980 990 1000
QELFAANHLT VTTIANTTDW PAKTKAVGFT LNDQPDNPVA IAALNHGQPL
1010 1020
ITAIDTLKTL LASTTTMVSE
Length:1,020
Mass (Da):108,993
Last modified:October 5, 2010 - v1
Checksum:iF6B52729D7DD2E71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACGZ02000027 Genomic DNA Translation: EFK28642.1
RefSeqiWP_003642067.1, NZ_GL379763.1

Genome annotation databases

EnsemblBacteriaiEFK28642; EFK28642; HMPREF0531_12320
PATRICifig|525338.16.peg.959

Similar proteinsi

Entry informationi

Entry nameiD7VDU7_LACPN
AccessioniPrimary (citable) accession number: D7VDU7
Entry historyiIntegrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: March 28, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health