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Protein

Alpha-amylase

Gene

VIT_03s0063g00450

Organism
Vitis vinifera (Grape)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202NucleophileUniRule annotation1
Active sitei227Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Ordered Locus Names:VIT_03s0063g00450Imported
OrganismiVitis vinifera (Grape)Imported
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis
Proteomesi
  • UP000009183 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_500310615323 – 425Alpha-amylaseSequence analysisAdd BLAST403

Proteomic databases

PRIDEiD7TPJ1.

Interactioni

Protein-protein interaction databases

STRINGi29760.VIT_03s0063g00450.t01.

Structurei

3D structure databases

ProteinModelPortaliD7TPJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 368AamyInterPro annotationAdd BLAST345
Domaini363 – 424Alpha-amyl_C2InterPro annotationAdd BLAST62

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
InParanoidiD7TPJ1.
KOiK01176.
OMAiMAKHSTT.
OrthoDBiEOG093600FS.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

D7TPJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIATSLSCL LFFITILPAL TASPILFQGF NWESSKKEGG WYNFLINSIP
60 70 80 90 100
ELAASGITHV WLPPPSQSVS PEGYMPGRLY DLNASKYGTQ DELKTLIKVF
110 120 130 140 150
HSKGVQCIAD IVINHRTAEK QDARGIWAIF EGGTPDDRLD WTPSFICKDD
160 170 180 190 200
TPYSDGTGNP DSGDDYSAAP DIDHINPRVQ QELIDWMNWL KIEIGFDGWR
210 220 230 240 250
FDFARGFSPA FTKFYMAKTR PKFAVGEIWK SLSYRSDGKP SYNQDSHRRE
260 270 280 290 300
LVEWVRGAGG AVNAFDFTTK GILQAAVEGE LWRMKDLNGK PPGMIGLMPG
310 320 330 340 350
NAVTFIDNHD TGSTLQHWPF PSDKVMQGYA YILTHPGIPS IFYDHFFEWG
360 370 380 390 400
LKEEIMKLII IRSRNRIKPN SAVRILASDS DLYVAAIDGK IIVKIGPRFD
410 420
VGNLVPKSFK KIATSGKDYC VWEKK
Length:425
Mass (Da):47,779
Last modified:August 10, 2010 - v1
Checksum:iF9156B3487F485A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN596006 Genomic DNA. Translation: CBI32414.3.
RefSeqiXP_002282184.1. XM_002282148.2.

Genome annotation databases

EnsemblPlantsiVIT_03s0063g00450.t01; VIT_03s0063g00450.t01; VIT_03s0063g00450.
GeneIDi100250317.
GrameneiVIT_03s0063g00450.t01; VIT_03s0063g00450.t01; VIT_03s0063g00450.
KEGGivvi:100250317.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN596006 Genomic DNA. Translation: CBI32414.3.
RefSeqiXP_002282184.1. XM_002282148.2.

3D structure databases

ProteinModelPortaliD7TPJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi29760.VIT_03s0063g00450.t01.

Proteomic databases

PRIDEiD7TPJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiVIT_03s0063g00450.t01; VIT_03s0063g00450.t01; VIT_03s0063g00450.
GeneIDi100250317.
GrameneiVIT_03s0063g00450.t01; VIT_03s0063g00450.t01; VIT_03s0063g00450.
KEGGivvi:100250317.

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
InParanoidiD7TPJ1.
KOiK01176.
OMAiMAKHSTT.
OrthoDBiEOG093600FS.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiD7TPJ1_VITVI
AccessioniPrimary (citable) accession number: D7TPJ1
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: October 5, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.