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Protein

Alpha-amylase

Gene

VIT_03s0063g00400

Organism
Vitis vinifera (Grape)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Cofactori

Ca2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202NucleophileUniRule annotation1
Active sitei227Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolaseUniRule annotation
Gene namesi
Ordered Locus Names:VIT_03s0063g00400Imported
OrganismiVitis vinifera (Grape)Imported
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis
Proteomesi
  • UP000009183 Componenti: Chromosome 3

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 27HelicalSequence analysisAdd BLAST22

Keywords - Cellular componenti

Membrane

PTM / Processingi

Proteomic databases

PRIDEiD7TPI6

Expressioni

Gene expression databases

ExpressionAtlasiD7TPI6 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi29760.VIT_03s0063g00400.t01

Structurei

3D structure databases

ProteinModelPortaliD7TPI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 362AamyInterPro annotationAdd BLAST339
Domaini363 – 423Alpha-amyl_C2InterPro annotationAdd BLAST61

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Phylogenomic databases

eggNOGiKOG0471 Eukaryota
COG0366 LUCA
InParanoidiD7TPI6
KOiK01176
OMAiGIPCLFW
OrthoDBiEOG093600FS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

D7TPI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGITTSLSYL LFFNIILPTL TASPILFQGF NWESSKKQGG WYNFLINSIP
60 70 80 90 100
ELSASGITHV WLPPPSQSAA SEGYLPGRLY DLNASHYGTQ YELKALIKAF
110 120 130 140 150
RSNGIQCIAD IVINHRTAEK KDSRGIWAIF EGGTPDDRLD WGPSFICSDD
160 170 180 190 200
TLFSDGTGNP DTGAGFDPAP DIDHVNPRVQ RELSDWMNWL KIEIGFAGWR
210 220 230 240 250
FDFARGYSPD FTKLYMENTS PNFAVGEIWN SLSYGNDSKP NYNQDAHRRE
260 270 280 290 300
LVDWVKAAGG AVTAFDFTTK GILQAAVEGE LWRLKDSNGG PPGMIGLMPE
310 320 330 340 350
NAVTFIDNHD TGSTQKIWPF PSDKVMQGYV YILTHPGIPS IFYDHFFDWG
360 370 380 390 400
LKEEISKLIS IRTRNGIKPN SVVRILASDP DLYVAAIDEK IIAKIGPRYD
410 420
VGNLVPSTFK LATSGNNYAV WEKQ
Length:424
Mass (Da):47,243
Last modified:August 10, 2010 - v1
Checksum:i34B563703DB1A850
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN596006 Genomic DNA Translation: CBI32409.3
RefSeqiXP_002285213.1, XM_002285177.3

Genome annotation databases

EnsemblPlantsiVIT_03s0063g00400.t01; VIT_03s0063g00400.t01; VIT_03s0063g00400
GeneIDi100245165
GrameneiVIT_03s0063g00400.t01; VIT_03s0063g00400.t01; VIT_03s0063g00400
KEGGivvi:100245165

Similar proteinsi

Entry informationi

Entry nameiD7TPI6_VITVI
AccessioniPrimary (citable) accession number: D7TPI6
Entry historyiIntegrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: March 28, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health