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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (ARALYDRAFT_492381), Glucose-6-phosphate isomerase (ARALYDRAFT_494761), Glucose-6-phosphate isomerase (PGIC), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase (ARALYDRAFT_490667), Fructose-bisphosphate aldolase (ARALYDRAFT_484021), Fructose-bisphosphate aldolase (ARALYDRAFT_900547), Fructose-bisphosphate aldolase (ARALYDRAFT_492157), Fructose-bisphosphate aldolase (ARALYDRAFT_482625), Fructose-bisphosphate aldolase (ARALYDRAFT_492160), Fructose-bisphosphate aldolase (ARALYDRAFT_906673)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102ATP; via amide nitrogenUniRule annotation1
Metal bindingi191Magnesium; catalyticUniRule annotation1
Sitei192Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei221Proton acceptorUniRule annotation1
Binding sitei320SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 166ATPUniRule annotation2
Nucleotide bindingi190 – 193ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFKUniRule annotation
ORF Names:ARALYDRAFT_913590Imported
OrganismiArabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress)Imported
Taxonomic identifieri81972 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000008694 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 400PFKInterPro annotationAdd BLAST304

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 221Substrate bindingUniRule annotation3
Regioni264 – 266Substrate bindingUniRule annotation3
Regioni376 – 379Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OrthoDBiEOG093609PL.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

D7MD40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSVSNSDR KIVTGPAGYI LEDVPHFTDD FPDHPTYPNP LQDNAAYSVV
60 70 80 90 100
KQYFVDEDDT VPQKIVVHPD SPRGTHFRRA GPRQRVYFES DDVLACIVTC
110 120 130 140 150
GGLCPGLNTV IREIVCGLSY MYGVKRILGI DGGYRGFYAR NTIHLDLKTV
160 170 180 190 200
NDIHRTGGTI LGTSRGGHDT TKIVDSIQDR GINQVYIIGG DGSQKGAAAI
210 220 230 240 250
FKEIRKRGLK VAVAGIPKTI DNDIPIIDKS FGFDTAVEEA QRAINAAHVE
260 270 280 290 300
ATSVENGIGL VKLMGRYSGF IAMYATLASR DVDCCLIPES PFFLEGPGGL
310 320 330 340 350
FEFIDKRLKE SGHMVIVIAE GAGQDLLAES MEESTTLKDA SGNKLLQDIG
360 370 380 390 400
LWISQRIKDH FAKKMTLTLK YIDPTYMIRA IPSNASDNVC CTLLAQSAVH
410 420 430 440 450
GVMAGYNGFT VGLVNGRHTY IPFYRITEKQ NKVVITDRMW ARLLSSTNQP
460 470
SFMKQDKIHS NQLVGEPGTM KW
Length:472
Mass (Da):51,875
Last modified:August 10, 2010 - v1
Checksum:i900AD448FDE7FA3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL348719 Genomic DNA. Translation: EFH43679.1.
RefSeqiXP_002867420.1. XM_002867374.1.

Genome annotation databases

EnsemblPlantsiscaffold_701332.1; scaffold_701332.1; scaffold_701332.1.
GeneIDi9305518.
Grameneiscaffold_701332.1; scaffold_701332.1; scaffold_701332.1.
KEGGialy:ARALYDRAFT_913590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL348719 Genomic DNA. Translation: EFH43679.1.
RefSeqiXP_002867420.1. XM_002867374.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiscaffold_701332.1; scaffold_701332.1; scaffold_701332.1.
GeneIDi9305518.
Grameneiscaffold_701332.1; scaffold_701332.1; scaffold_701332.1.
KEGGialy:ARALYDRAFT_913590.

Phylogenomic databases

KOiK00850.
OrthoDBiEOG093609PL.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiD7MD40_ARALL
AccessioniPrimary (citable) accession number: D7MD40
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: November 2, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.