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Protein

Ribulose bisphosphate carboxylase large chain

Gene

rbcL

Organism
Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123Substrate; in homodimeric partnerUniRule annotation1
Binding sitei173SubstrateUniRule annotation1
Active sitei175Proton acceptorUniRule annotation1
Binding sitei177SubstrateUniRule annotation1
Metal bindingi201Magnesium; via carbamate groupUniRule annotation1
Metal bindingi203MagnesiumUniRule annotation1
Metal bindingi204MagnesiumUniRule annotation1
Active sitei294Proton acceptorUniRule annotation1
Binding sitei295SubstrateUniRule annotation1
Binding sitei327SubstrateUniRule annotation1
Sitei334Transition state stabilizerUniRule annotation1
Binding sitei379SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Calvin cycleUniRule annotation, Carbon dioxide fixationUniRule annotation, PhotorespirationUniRule annotation, PhotosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:rbcLUniRule annotationImported
ORF Names:ARALYDRAFT_475563Imported
Encoded oniPlastid; ChloroplastImported
OrganismiArabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress)Imported
Taxonomic identifieri81972 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000008694 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Plastidchloroplast UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

ChloroplastUniRule annotationImported, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_50084414231 – 2UniRule annotation2
ChainiPRO_50084414243 – 479Ribulose bisphosphate carboxylase large chainUniRule annotationAdd BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N-acetylprolineUniRule annotation1
Modified residuei14N6,N6,N6-trimethyllysineUniRule annotation1
Modified residuei201N6-carboxylysineUniRule annotation1
Disulfide bondi247Interchain; in linked formUniRule annotation

Post-translational modificationi

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation, Disulfide bondUniRule annotation, MethylationUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 144RuBisCO_large_NInterPro annotationAdd BLAST121
Domaini154 – 462RuBisCO_largeInterPro annotationAdd BLAST309

Phylogenomic databases

KOiK01601.
OrthoDBiEOG093607MT.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D7KRA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPQTETKAS VGFKAGVKEY KLTYYTPEYE TKDTDILAAF RVTPQPGVPP
60 70 80 90 100
EEAGAAVAAE SSTGTWTTVW TDGLTSLDRY KGRCYHIEPV PGEETQFIAY
110 120 130 140 150
VAYPLDLFEE GSVTNMFTSI VGNVFGFKAL AALRLEDLRI PPAYTKTFQG
160 170 180 190 200
PPHGIQVERD KLNKYGRPLL GCTIKPKLGL SAKNYGRAVY ECLRGGLDFT
210 220 230 240 250
KDDENVNSQP FMRWRDRFLF CAEAIYKSQA ETGEIKGHYL NATAGTCEEM
260 270 280 290 300
IKRAVFAREL GVPIVMHDYL TGGFTANTSL AHYCRDNGLL LHIHRAMHAV
310 320 330 340 350
IDRQKNHGMH FRVLAKALRL SGGDHVHAGT VVGKLEGDRE STLGFVDLLR
360 370 380 390 400
DDYVEKDRSR GIFFTQDWVS LPGVLPVASG GIHVWHMPAL TEIFGDDSVL
410 420 430 440 450
QFGGGTLGHP WGNAPGAVAN RVALEACVQA RNEGRDLAVE GNEIIREACK
460 470
WSPELAAACE VWKEIRFNFP TVDKLDGQE
Length:479
Mass (Da):52,966
Last modified:August 10, 2010 - v1
Checksum:i397540B86771A5DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LN877383 Genomic DNA. Translation: CUA65412.1.
LT161942 Genomic DNA. Translation: CZF91239.1.
GL348714 Genomic DNA. Translation: EFH64608.1.
RefSeqiXP_002888349.1. XM_002888303.1.

Genome annotation databases

EnsemblPlantsifgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1.
GeneIDi9324412.
Grameneifgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1.
KEGGialy:ARALYDRAFT_475563.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LN877383 Genomic DNA. Translation: CUA65412.1.
LT161942 Genomic DNA. Translation: CZF91239.1.
GL348714 Genomic DNA. Translation: EFH64608.1.
RefSeqiXP_002888349.1. XM_002888303.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsifgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1.
GeneIDi9324412.
Grameneifgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1; fgenesh2_kg.2__693__ATCG00490.1.
KEGGialy:ARALYDRAFT_475563.

Phylogenomic databases

KOiK01601.
OrthoDBiEOG093607MT.

Family and domain databases

CDDicd08212. RuBisCO_large_I. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiD7KRA6_ARALL
AccessioniPrimary (citable) accession number: D7KRA6
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: November 30, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.