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Protein

Thymidylate synthase

Gene

thyA

Organism
Vibrio cholerae RC385
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22dUMPUniRule annotation1
Active sitei160PROSITE-ProRule annotation1
Active sitei160NucleophileUniRule annotation1
Binding sitei1835,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei191dUMPUniRule annotation1
Binding sitei2825,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 183dUMPUniRule annotation4
Nucleotide bindingi221 – 223dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:VCRC385_03343Imported
OrganismiVibrio cholerae RC385Imported
Taxonomic identifieri345074 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000004696 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD7HD57
SMRiD7HD57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 283Thymidylat_syntInterPro annotationAdd BLAST281

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.101 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

D7HD57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQYLDLCQR IVDQGVWVEN ERTGKRCLTV INADLTYDVG NNQFPLVTTR
60 70 80 90 100
KSFWKAAVAE LLGYIRGYDN AADFRQLGTK TWDANANLNQ AWLNNPYRKG
110 120 130 140 150
EDDMGRVYGV QGRAWAKPDG GHIDQLKKIV DDLSRGVDDR GEILNFYNPG
160 170 180 190 200
EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR SCDVPLGLNF NMVQVYVFLA
210 220 230 240 250
LMAQITGKKP GLAYHKIVNA HIYQDQLELM RDVQLKREPF PAPQFHINPK
260 270 280
IKTLQDLETW VTLDDFDVTG YQFHDPIQYP FSV
Length:283
Mass (Da):32,573
Last modified:August 10, 2010 - v1
Checksum:iD50B760285FDE97E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG774557 Genomic DNA Translation: EFH74050.1
RefSeqiWP_001250353.1, NZ_GG774557.1

Genome annotation databases

EnsemblBacteriaiEFH74050; EFH74050; VCRC385_03343
PATRICifig|345074.23.peg.1895

Similar proteinsi

Entry informationi

Entry nameiD7HD57_VIBCL
AccessioniPrimary (citable) accession number: D7HD57
Entry historyiIntegrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: February 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported