thyA

Functionⁱ

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityⁱ

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayⁱ: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	22	dUMPUniRule annotation	1
Active siteⁱ	160	PROSITE-ProRule annotation	1
Active siteⁱ	160	NucleophileUniRule annotation	1
Binding siteⁱ	183	5,10-methylenetetrahydrofolateUniRule annotation	1
Binding siteⁱ	191	dUMPUniRule annotation	1
Binding siteⁱ	282	5,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	180 – 183	dUMPUniRule annotation		4
Nucleotide bindingⁱ	221 – 223	dUMPUniRule annotation		3

GO - Molecular functionⁱ

thymidylate synthase activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

dTMP biosynthetic process Source: UniProtKB-UniRule
dTTP biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	MethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological process	Nucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayⁱ	UPA00575

UniPathwayⁱ

UPA00575

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation) Short name: TSUniRule annotation Short name: TSaseUniRule annotation
Gene namesⁱ	Name:thyAUniRule annotation ORF Names:VCRC385_03343Imported
Organismⁱ	Vibrio cholerae RC385Imported
Taxonomic identifierⁱ	345074 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae
Proteomesⁱ	UP000004696 Componentⁱ: Unassembled WGS sequence

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_00008

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

CytoplasmUniRule annotation

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotationSAAS annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	D7HD57
SMRⁱ	D7HD57
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	3 – 283	Thymidylat_syntInterPro annotationAdd BLAST		281

Sequence similaritiesⁱ

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Dⁱ	3.30.572.101 hit
HAMAPⁱ	MF_00008 Thymidy_synth_bact, 1 hit
InterProⁱ	View protein in InterPro IPR023451 Thymidate_synth/dCMP_Mease IPR036926 Thymidate_synth/dCMP_Mease_sf IPR000398 Thymidylate_synthase IPR020940 Thymidylate_synthase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303 Thymidylat_synt, 1 hit
PRINTSⁱ	PR00108 THYMDSNTHASE
SUPFAMⁱ	SSF55831 SSF55831, 1 hit
TIGRFAMsⁱ	TIGR03284 thym_sym, 1 hit
PROSITEⁱ	View protein in PROSITE PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

D7HD57-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRQYLDLCQR IVDQGVWVEN ERTGKRCLTV INADLTYDVG NNQFPLVTTR 
        60         70         80         90        100
KSFWKAAVAE LLGYIRGYDN AADFRQLGTK TWDANANLNQ AWLNNPYRKG 
       110        120        130        140        150
EDDMGRVYGV QGRAWAKPDG GHIDQLKKIV DDLSRGVDDR GEILNFYNPG 
       160        170        180        190        200
EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR SCDVPLGLNF NMVQVYVFLA 
       210        220        230        240        250
LMAQITGKKP GLAYHKIVNA HIYQDQLELM RDVQLKREPF PAPQFHINPK 
       260        270        280 
IKTLQDLETW VTLDDFDVTG YQFHDPIQYP FSV

Length:283

Mass (Da):32,573

Last modified:August 10, 2010 - v1

Checksum:ⁱD50B760285FDE97E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	GG774557 Genomic DNA Translation: EFH74050.1
NCBI Reference Sequences More...RefSeqⁱ	WP_001250353.1, NZ_GG774557.1

Genome annotation databases

EnsemblBacteriaⁱ	EFH74050; EFH74050; VCRC385_03343
PATRICⁱ	fig\|345074.23.peg.1895

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D7HD57	O66108 C3LST0 A0A2L2M2W3 A0A0H6NUW5 A0A0X1L3L6 A0A0H3QAH6 A0A0K9UUG6	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Vibrio cholerae serotype O1 (strain M66-2) Vibrio cholerae O1 biovar El Tor Vibrio cholerae Vibrio cholerae (strain MO10) Vibrio cholerae B33 Vibrio cholerae 2740-80	283	UniRef100_O66108	Cluster: Thymidylate synthase	8

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D7HD57	O66108 C3LST0 A0A2L2M2W3 A0A0H6NUW5 A0A0X1L3L6 A0A0H3QAH6 A0A0K9UUG6 A0A0H6QST8 A0A0H6JJ38 A0A0H5ZZM5 +175	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Vibrio cholerae serotype O1 (strain M66-2) Vibrio cholerae O1 biovar El Tor Vibrio cholerae Vibrio cholerae (strain MO10) Vibrio cholerae B33 Vibrio cholerae 2740-80 Vibrio metoecus Vibrio sp. JCM 18905 Vibrio antiquarius (strain Ex25) And more	283	UniRef90_O66108	Cluster: Thymidylate synthase	186

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
D7HD57	O66108 C3LST0 A0A2L2M2W3 A0A0H6NUW5 A0A0X1L3L6 A0A0H3QAH6 A0A0K9UUG6 A0A0H6QST8 A0A0H6JJ38 A0A0H5ZZM5 +996	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Vibrio cholerae serotype O1 (strain M66-2) Vibrio cholerae O1 biovar El Tor Vibrio cholerae Vibrio cholerae (strain MO10) Vibrio cholerae B33 Vibrio cholerae 2740-80 Vibrio sp. JCM 18905 Vibrio antiquarius (strain Ex25) Vibrio parahaemolyticus And more	283	UniRef50_O66108	Cluster: Thymidylate synthase	1,007

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	GG774557 Genomic DNA Translation: EFH74050.1
NCBI Reference Sequences More...RefSeqⁱ	WP_001250353.1, NZ_GG774557.1

3D structure databases

ProteinModelPortalⁱ	D7HD57
SMRⁱ	D7HD57
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	EFH74050; EFH74050; VCRC385_03343
PATRICⁱ	fig\|345074.23.peg.1895

Enzyme and pathway databases

UniPathwayⁱ	UPA00575

UniPathwayⁱ

UPA00575

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Dⁱ	3.30.572.101 hit
HAMAPⁱ	MF_00008 Thymidy_synth_bact, 1 hit
InterProⁱ	View protein in InterPro IPR023451 Thymidate_synth/dCMP_Mease IPR036926 Thymidate_synth/dCMP_Mease_sf IPR000398 Thymidylate_synthase IPR020940 Thymidylate_synthase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303 Thymidylat_synt, 1 hit
PRINTSⁱ	PR00108 THYMDSNTHASE
SUPFAMⁱ	SSF55831 SSF55831, 1 hit
TIGRFAMsⁱ	TIGR03284 thym_sym, 1 hit
PROSITEⁱ	View protein in PROSITE PS00091 THYMIDYLATE_SYNTHASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	D7HD57_VIBCL
Accessionⁱ	D7HD57Primary (citable) accession number: D7HD57
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	August 10, 2010
	Last sequence update:	August 10, 2010
	Last modified:	February 28, 2018
	This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteomeImported

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - D7HD57 (D7HD57_VIBCL)

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Thymidylate synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: dTTP biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: dTTP biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ