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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Vibrio cholerae RC385
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotationSAAS annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotationSAAS annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotationSAAS annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotationSAAS annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei135Important for catalytic activityUniRule annotation1
Sitei157Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotation, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation
Biological processFatty acid metabolismUniRule annotationSAAS annotation, Lipid degradationUniRule annotationSAAS annotation, Lipid metabolism
LigandNADUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
ORF Names:VCRC385_01854Imported
OrganismiVibrio cholerae RC385Imported
Taxonomic identifieri345074 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000004696 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD7H7P7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini332 – 5113HCDH_NInterPro annotationAdd BLAST180
Domaini513 – 6063HCDHInterPro annotationAdd BLAST94
Domaini635 – 7103HCDHInterPro annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 207Enoyl-CoA hydrataseUniRule annotationAdd BLAST207
Regioni327 – 7243-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST398

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotationSAAS annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

D7H7P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCCGWLRRCY DSGGGIMDNN NAFQLSFDEQ HYAWLAIDVP GEKMNTLQAA
60 70 80 90 100
FAEEMQAVFA TLNEKRGQIK GLIIHSLKPD NFIAGADVRM LEACQSVHEA
110 120 130 140 150
QALASQGQQM FQQLADLPFP VVAAIHGPCL GGGLELALAC DYRVCTEDEV
160 170 180 190 200
TRLGLPEVML GLLPGSGGTQ RLPRLIGLLP ALDLILTGKQ LRAKKAKKLG
210 220 230 240 250
VVDACVPHSV LLDVAKRLLE EKGHKKRAQV TLPIKEKLLA NTGLGRKLIF
260 270 280 290 300
DQAAKKTQQK TRGNYPAAQA ILEVIQYGLE KGMHAGLEYE AKRFAELVMT
310 320 330 340 350
RESKALRSIF FATTEMKKDL GADAKPAPVA AVGVLGGGLM GAGISHVTVA
360 370 380 390 400
KAKTSVRIKD VANDGVLNAL NYNYKLFDKQ RQRKILTKAQ LQAQMSQLSG
410 420 430 440 450
GTGFVGFDRC DVVIEAVFED LKLKQQMVAD IEANAKPTTI FATNTSSLPI
460 470 480 490 500
HQIASQAQRP QNIVGLHYFS PVEKMPLVEV IPHATTSDET IATVVTLARK
510 520 530 540 550
QGKTPIVVKD CAGFYVNRIL APYMNEAAQV LMAGEPIEKL DAALLDFGFP
560 570 580 590 600
VGPITLLDEV GVDIGAKIMP ILVKELGPRF QGPDVFDVLL KDNRKGRKSG
610 620 630 640 650
KGFYTYKGSK KKEVDKSVYK LLKLTPESKL NDKEIAMRCL LPMLNEAVRC
660 670 680 690 700
LDEGIIRSAR DGDMGAIFGI GFPPFLGGPF RYMDTLGLTK VVEMMNQHTE
710 720
KYGERFAPCD GLLTRAGLGE KFYP
Length:724
Mass (Da):79,058
Last modified:August 10, 2010 - v1
Checksum:i4BC8FB9F2B278168
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG774555 Genomic DNA Translation: EFH75078.1

Genome annotation databases

EnsemblBacteriaiEFH75078; EFH75078; VCRC385_01854
PATRICifig|345074.23.peg.3004

Similar proteinsi

Entry informationi

Entry nameiD7H7P7_VIBCL
AccessioniPrimary (citable) accession number: D7H7P7
Entry historyiIntegrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: March 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported