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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanohalobium evestigatum (strain DSM 3721 / OCM 161 / Z-7303)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei151 – 1511Proton acceptorUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation
Metal bindingi177 – 1771Magnesium; via carbamate groupUniRule annotation
Metal bindingi179 – 1791MagnesiumUniRule annotation
Metal bindingi180 – 1801MagnesiumUniRule annotation
Active sitei270 – 2701Proton acceptorUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation
Binding sitei303 – 3031SubstrateUniRule annotation
Sitei310 – 3101Transition state stabilizerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMEVE644295:GHG3-938-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Metev_0915Imported
OrganismiMethanohalobium evestigatum (strain DSM 3721 / OCM 161 / Z-7303)Imported
Taxonomic identifieri644295 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanohalobium
ProteomesiUP000000391 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei177 – 1771N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi644295.Metev_0915.

Structurei

3D structure databases

ProteinModelPortaliD7E762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni354 – 3563Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

D7E762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREDYIETEY KPKPDELVCE YYMEPADGVS FEKACNHMAG ESSIDTWSDI
60 70 80 90 100
STLSPEKAQE LKPHVFSIDK ERGVVKVAYK QDLFEIDSVP QILSAIAGNI
110 120 130 140 150
MSMKLVKNLR LEDIAFPKDV LNSFRGPKYG LNGVRDLFGV YDRPLVGTIV
160 170 180 190 200
KPKVGLSSEK HADVAYKSFV GGCDIVKDDE NLTNQKFNAF DKRVKITLDA
210 220 230 240 250
KEKAEKETGE RKMYLCNITA PTCEEMLRRA EVINDLGGKY VMIDIITAGW
260 270 280 290 300
SALQSLREAT EDMDIAIHAH RCMHSVMTRN PRHGVNMVAL AKLTRLIGHD
310 320 330 340 350
QLHIGTVVGK MHGDKDEVLS LRDECALDYM PANDQLHILE QDWGNIKPMM
360 370 380 390 400
PVASGGLEPG MIPDLYNMFG KDVIMQFGAG IHAHPMGTEA GATACRQSVE
410 420
AALENITLEE YASNHKELQS ALEKWG
Length:426
Mass (Da):47,523
Last modified:August 10, 2010 - v1
Checksum:i325E9C3FBB4FB0AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002069 Genomic DNA. Translation: ADI73811.1.
RefSeqiWP_013194379.1. NC_014253.1.
YP_003726607.1. NC_014253.1.

Genome annotation databases

EnsemblBacteriaiADI73811; ADI73811; Metev_0915.
GeneIDi9346544.
KEGGimev:Metev_0915.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002069 Genomic DNA. Translation: ADI73811.1.
RefSeqiWP_013194379.1. NC_014253.1.
YP_003726607.1. NC_014253.1.

3D structure databases

ProteinModelPortaliD7E762.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi644295.Metev_0915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADI73811; ADI73811; Metev_0915.
GeneIDi9346544.
KEGGimev:Metev_0915.

Phylogenomic databases

HOGENOMiHOG000230831.
KOiK01601.
OMAiFTQDWAS.

Enzyme and pathway databases

BioCyciMEVE644295:GHG3-938-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence chromosome of Methanohalobium evestigatum Z-7303."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Saunders E., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Anderson I., Woyke T.
    Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 3721 / OCM 161 / Z-7303Imported.

Entry informationi

Entry nameiD7E762_METEZ
AccessioniPrimary (citable) accession number: D7E762
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: July 22, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.