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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Nostoc azollae (strain 0708) (Anabaena azollae (strain 0708))
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mg2+SAAS annotation, Mn2+SAAS annotationNote: Binds 2 magnesium or manganese ions per subunit.SAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi296 – 2961Magnesium or manganese 1UniRule annotation
Metal bindingi310 – 3101Magnesium or manganese 1UniRule annotation
Metal bindingi310 – 3101Magnesium or manganese 2UniRule annotation
Metal bindingi312 – 3121Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi170 – 22556ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. D-alanine-D-alanine ligase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  3. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotation

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciTAZO551115:GH0O-272-MONOMER.
UniPathwayiUPA00219.
UPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:Aazo_0272Imported
OrganismiNostoc azollae (strain 0708) (Anabaena azollae (strain 0708))Imported
Taxonomic identifieri551115 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeTrichormus
ProteomesiUP000001511: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD7DYP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 343210ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation
Contains ATP-grasp domain.SAAS annotation

Phylogenomic databases

HOGENOMiHOG000011593.
KOiK01921.
OMAiWFPILHG.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D7DYP2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKLRVGLLF GGRSGEHEVS IISARGIANA LSAEENSHKY EIMPFYIQKD
60 70 80 90 100
GFWQAGEVAQ QVLTSGVAKE HPGNRANLWQ FPPQTAEIDL WFPILHGPNG
110 120 130 140 150
EDGTIQALLT LMQVPFVGSG VLGSATGMDK IAMKTAFAQA GLPQVKYKAL
160 170 180 190 200
TRAQIWSNPC VFPKLCDEIE ATLGYPYFVK PANLGSSVGI AKVQSRQELE
210 220 230 240 250
AALDNAATYD RRIIVEAGVV AREVECAVLG NDNPKASVIG EITFDSDFYD
260 270 280 290 300
YETKYTQGKA DLFIPAHLPE SAVQQIQEKA LQAFTAVDAA GLARVDFFYV
310 320 330 340 350
EATGEILINE INTLPGFTST SMYPQLWANT GISFPKLVDQ LIQLAMERHS

N
Length:351
Mass (Da):38,306
Last modified:August 10, 2010 - v1
Checksum:i3946D7BA9DC96E23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002059 Genomic DNA. Translation: ADI62865.1.
RefSeqiYP_003719988.1. NC_014248.1.

Genome annotation databases

EnsemblBacteriaiADI62865; ADI62865; Aazo_0272.
GeneIDi9338056.
KEGGinaz:Aazo_0272.
PATRICi38222109. VBINosAzo102301_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002059 Genomic DNA. Translation: ADI62865.1.
RefSeqiYP_003719988.1. NC_014248.1.

3D structure databases

ProteinModelPortaliD7DYP2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADI62865; ADI62865; Aazo_0272.
GeneIDi9338056.
KEGGinaz:Aazo_0272.
PATRICi38222109. VBINosAzo102301_0369.

Phylogenomic databases

HOGENOMiHOG000011593.
KOiK01921.
OMAiWFPILHG.

Enzyme and pathway databases

UniPathwayiUPA00219.
UPA00219.
BioCyciTAZO551115:GH0O-272-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome erosion in a nitrogen-fixing vertically transmitted endosymbiotic multicellular cyanobacterium."
    Ran L., Larsson J., Vigil-Stenman T., Nylander J.A., Ininbergs K., Zheng W.W., Lapidus A., Lowry S., Haselkorn R., Bergman B.
    PLoS ONE 5:E11486-E11486(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 0708Imported.

Entry informationi

Entry nameiD7DYP2_NOSA0
AccessioniPrimary (citable) accession number: D7DYP2
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: February 4, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.