Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA ligase

Gene

lig

Organism
Staphylothermus hellenicus (strain DSM 12710 / JCM 10830 / BK20S6-10-b1 / P8)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei276 – 2761ATPUniRule annotation
Active sitei278 – 2781N6-AMP-lysine intermediateUniRule annotation
Binding sitei283 – 2831ATPUniRule annotation
Binding sitei298 – 2981ATPUniRule annotation
Binding sitei328 – 3281ATPUniRule annotation
Binding sitei368 – 3681ATPUniRule annotation
Binding sitei445 – 4451ATPUniRule annotation
Binding sitei451 – 4511ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSHEL591019:GC4A-1151-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Shell_1131Imported
OrganismiStaphylothermus hellenicus (strain DSM 12710 / JCM 10830 / BK20S6-10-b1 / P8)Imported
Taxonomic identifieri591019 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeStaphylothermus
ProteomesiUP000002573 Componenti: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000036008.
KOiK10747.
OMAiLACYDEQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D7D8Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQQNEQPR YEKDMPFREL ADTFERIEVI TSRTQMTVLL VNLFKKTPPE
60 70 80 90 100
IIDKVVYLLQ GRLWPDWKGL PELGVGEKML VKAIALATQS RESEVESLYK
110 120 130 140 150
SLGDLGKAAE KLKAKYEEKL KKGAMSILAF VPAKKELTVG QVYETLSRVA
160 170 180 190 200
LATGEGSRDI KLKLLAGLLS DASPKEAKYI IRFVEGRLRL GIGDATIMDA
210 220 230 240 250
LAIVYGGGAH ARPIVERAYN LRADLGHIAK ILATQGLEAL KGIKPQVGIP
260 270 280 290 300
IRPMLAERLN NPVEILKKVG GDSFVEYKYD GERAQIHKLG DKIWIYSRRL
310 320 330 340 350
ENITHQYPDV VDYARKYIKA DEAIVEGEIV AYDPDTGELR PFQELMHRKR
360 370 380 390 400
KHDIHIAIKE VPVKVFLFDL LYVDGEDYTL KPLPERRAKL EEIIEQTETF
410 420 430 440 450
QIAEYIRTND PDELEKFFFK AIEDGAEGVM IKALHKNAIY QAGTRGWLWI
460 470 480 490 500
KYKRDYKSEM IDTVDLVIIG AFYGRGRRGG KYGALLMASY NPDKDVFESV
510 520 530 540 550
CKVGSGFKDE DIDKLPEMLK PYIIEHKHPR VVARMKPDIW VTPALVAEII
560 570 580 590 600
GAELTLSPLH TCCLDAIKPG VGISIRFPRF IRWRPDKGPE DATTSQELLE
610
MYKRQLKKLS E
Length:611
Mass (Da):69,402
Last modified:August 10, 2010 - v1
Checksum:iD0B0E2C2548555CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002051 Genomic DNA. Translation: ADI32232.1.
RefSeqiYP_003669131.1. NC_014205.1.

Genome annotation databases

EnsemblBacteriaiADI32232; ADI32232; Shell_1131.
GeneIDi9234420.
KEGGishc:Shell_1131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002051 Genomic DNA. Translation: ADI32232.1.
RefSeqiYP_003669131.1. NC_014205.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADI32232; ADI32232; Shell_1131.
GeneIDi9234420.
KEGGishc:Shell_1131.

Phylogenomic databases

HOGENOMiHOG000036008.
KOiK10747.
OMAiLACYDEQ.

Enzyme and pathway databases

BioCyciSHEL591019:GC4A-1151-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Staphylothermus hellenicus DSM 12710."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Anderson I.J., Woyke T.
    Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 12710 / JCM 10830 / BK20S6-10-b1 / P8Imported.

Entry informationi

Entry nameiD7D8Y6_STAHD
AccessioniPrimary (citable) accession number: D7D8Y6
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: April 1, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.