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D7CPP1 (D7CPP1_SYNLT) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Protein attributes

Sequence length636 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01898 SAAS SAAS013506

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity. HAMAP-Rule MF_01898

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01898

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family. HAMAP-Rule MF_01898 RuleBase RU000380

Contains 1 Toprim domain. HAMAP-Rule MF_01898

Contains Toprim domain. SAAS SAAS011557

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain421 – 535115Toprim By similarity HAMAP-Rule MF_01898

Sites

Metal binding4271Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5001Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5001Magnesium 2 By similarity HAMAP-Rule MF_01898
Metal binding5021Magnesium 2 By similarity HAMAP-Rule MF_01898
Site4521Interaction with DNA By similarity HAMAP-Rule MF_01898
Site4551Interaction with DNA By similarity HAMAP-Rule MF_01898

Sequences

Sequence LengthMass (Da)Tools
D7CPP1 [UniParc].

Last modified August 10, 2010. Version 1.
Checksum: 0799A7E8050557BF

FASTA63671,076
        10         20         30         40         50         60 
MNINGSKNEY TALDIQVLEG LEAVRKRPGM YIGSTDSRGL HHLVFEIVDN SIDEAVAGYC 

        70         80         90        100        110        120 
DEIKVIILDD STVTVTDNGR GIPVDMHPKM GIPALEVILT TLHSGAKFGG SGYTISGGLH 

       130        140        150        160        170        180 
GVGLSVVNAL SRWLEVEVAR DGQLYHQRYE RGKPVTPLAV TGACQTTGTK ITFSPDPEIF 

       190        200        210        220        230        240 
EEICFSAEII GQRLRELSYL NQGTRIVLCD KRTGRDVEYY HTGGIRDFVK YLSKNREPLS 

       250        260        270        280        290        300 
PEPIYFRIGK EGTVVEVAIQ YNTGYTETLL SYANNIHTQE GGTHELGFKN GLTRVVNEYA 

       310        320        330        340        350        360 
KKQGMLKENE GNLLGEDIRE GMTAVISVLV QDPQFEGQTK TKLGNAFIRS LVEGAVSDFV 

       370        380        390        400        410        420 
GAFFDENPSL AKKIVEKAIA ARRAREAARK ARELTRRKNL LDSTSLPGKL ADCSEKDPAL 

       430        440        450        460        470        480 
SELYLVEGDS AGGSAKQGRD RNFQAILPLR GKILNVEKAR LDKVLSNEEI RALITAIGTG 

       490        500        510        520        530        540 
IGEDFDINKA RYHRLIIMTD ADVDGSHIRV LLLTFFYRYM RPLIEAGYVY VAQPPLYELK 

       550        560        570        580        590        600 
RGKEVHYIYN DRELQRFLEQ QGNKKYGVKR YKGLGEMNPE QLWETTMNPE NRTLLQVRIE 

       610        620        630 
DAIAADVIFS SLMGEKVEPR REFIQENARL VTNLDI

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002048 Genomic DNA. Translation: ADI00813.1.
RefSeqYP_003701378.1. NC_014220.1.

3D structure databases

ProteinModelPortalD7CPP1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADI00813; ADI00813; Slip_0005.
GeneID9272772.
KEGGslp:Slip_0005.
PATRIC38266332. VBISynLip21176_0006.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000075154.
KOK02470.
OMAHIIIMTD.

Enzyme and pathway databases

BioCycSLIP643648:GHUR-5-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPMF_01898. GyrB.
InterProIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01059. gyrB. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD7CPP1_SYNLT
AccessionPrimary (citable) accession number: D7CPP1
Entry history
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: June 11, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info