UniProtKB - D7CPP1 (D7CPP1_SYNLT)
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Protein
DNA gyrase subunit B
Gene
gyrB
Organism
Syntrophothermus lipocalidus (strain DSM 12680 / TGB-C1)
Status
Functioni
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation
Miscellaneous
Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation
Catalytic activityi
ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationSAAS annotation
Cofactori
Protein has several cofactor binding sites:GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-KW
- DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA-dependent DNA replication Source: UniProtKB-UniRule
- DNA topological change Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-bindingSAAS annotation, Isomerase, TopoisomeraseUniRule annotationSAAS annotation |
Ligand | ATP-bindingUniRule annotationSAAS annotation, MagnesiumSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | SLIP643648:G1GKX-5-MONOMER. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gyrBUniRule annotation Ordered Locus Names:Slip_0005Imported |
Organismi | Syntrophothermus lipocalidus (strain DSM 12680 / TGB-C1)Imported |
Taxonomic identifieri | 643648 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Clostridiales › Syntrophomonadaceae › Syntrophothermus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- chromosome Source: InterPro
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationInteractioni
Subunit structurei
Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation
Protein-protein interaction databases
STRINGi | 643648.Slip_0005. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 421 – 535 | ToprimInterPro annotationAdd BLAST | 115 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 377 – 397 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the type II topoisomerase family.UniRule annotation
Keywords - Domaini
Coiled coilSequence analysisPhylogenomic databases
eggNOGi | ENOG4105C7D. Bacteria. COG0187. LUCA. |
HOGENOMi | HOG000075154. |
KOi | K02470. |
OMAi | DCSSRDP. |
OrthoDBi | POG091H01XP. |
Family and domain databases
CDDi | cd00075. HATPase_c. 1 hit. cd03366. TOPRIM_TopoIIA_GyrB. 1 hit. |
Gene3Di | 3.30.230.10. 1 hit. 3.30.565.10. 1 hit. 3.40.50.670. 1 hit. |
HAMAPi | MF_01898. GyrB. 1 hit. |
InterProi | View protein in InterPro IPR002288. DNA_gyrase_B_C. IPR011557. GyrB. IPR003594. HATPase_C. IPR036890. HATPase_C_sf. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013760. Topo_IIA-like_dom_sf. IPR013759. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR018522. TopoIIA_CS. IPR006171. TOPRIM_domain. IPR034160. TOPRIM_GyrB. |
PANTHERi | PTHR10169. PTHR10169. 1 hit. |
Pfami | View protein in Pfam PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. |
SMARTi | View protein in SMART SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. |
SUPFAMi | SSF54211. SSF54211. 1 hit. SSF55874. SSF55874. 1 hit. SSF56719. SSF56719. 1 hit. |
TIGRFAMsi | TIGR01059. gyrB. 1 hit. |
PROSITEi | View protein in PROSITE PS00177. TOPOISOMERASE_II. 1 hit. PS50880. TOPRIM. 1 hit. |
i Sequence
Sequence statusi: Complete.
D7CPP1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNINGSKNEY TALDIQVLEG LEAVRKRPGM YIGSTDSRGL HHLVFEIVDN
60 70 80 90 100
SIDEAVAGYC DEIKVIILDD STVTVTDNGR GIPVDMHPKM GIPALEVILT
110 120 130 140 150
TLHSGAKFGG SGYTISGGLH GVGLSVVNAL SRWLEVEVAR DGQLYHQRYE
160 170 180 190 200
RGKPVTPLAV TGACQTTGTK ITFSPDPEIF EEICFSAEII GQRLRELSYL
210 220 230 240 250
NQGTRIVLCD KRTGRDVEYY HTGGIRDFVK YLSKNREPLS PEPIYFRIGK
260 270 280 290 300
EGTVVEVAIQ YNTGYTETLL SYANNIHTQE GGTHELGFKN GLTRVVNEYA
310 320 330 340 350
KKQGMLKENE GNLLGEDIRE GMTAVISVLV QDPQFEGQTK TKLGNAFIRS
360 370 380 390 400
LVEGAVSDFV GAFFDENPSL AKKIVEKAIA ARRAREAARK ARELTRRKNL
410 420 430 440 450
LDSTSLPGKL ADCSEKDPAL SELYLVEGDS AGGSAKQGRD RNFQAILPLR
460 470 480 490 500
GKILNVEKAR LDKVLSNEEI RALITAIGTG IGEDFDINKA RYHRLIIMTD
510 520 530 540 550
ADVDGSHIRV LLLTFFYRYM RPLIEAGYVY VAQPPLYELK RGKEVHYIYN
560 570 580 590 600
DRELQRFLEQ QGNKKYGVKR YKGLGEMNPE QLWETTMNPE NRTLLQVRIE
610 620 630
DAIAADVIFS SLMGEKVEPR REFIQENARL VTNLDI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002048 Genomic DNA. Translation: ADI00813.1. |
RefSeqi | WP_013174217.1. NC_014220.1. |
Genome annotation databases
EnsemblBacteriai | ADI00813; ADI00813; Slip_0005. |
KEGGi | slp:Slip_0005. |
Similar proteinsi
Entry informationi
Entry namei | D7CPP1_SYNLT | |
Accessioni | D7CPP1Primary (citable) accession number: D7CPP1 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | August 10, 2010 |
Last sequence update: | August 10, 2010 | |
Last modified: | February 28, 2018 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |