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D7CIA7 (D7CIA7_STRBB) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cyclic pyranopterin monophosphate synthase HAMAP-Rule MF_01225
EC=4.1.99.18 HAMAP-Rule MF_01225
Alternative name(s):
Molybdenum cofactor biosynthesis protein A HAMAP-Rule MF_01225
Gene names
Name:moaA HAMAP-Rule MF_01225
Ordered Locus Names:SBI_08167
OrganismStreptomyces bingchenggensis (strain BCW-1) [Complete proteome] [HAMAP] EMBL ADI11285.1
Taxonomic identifier749414 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z) By similarity. HAMAP-Rule MF_01225 SAAS SAAS013483

Catalytic activity

GTP = cyclic pyranopterin monophosphate + diphosphate. HAMAP-Rule MF_01225 SAAS SAAS013483

Cofactor

Binds 2 4Fe-4S clusters. Binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate By similarity. HAMAP-Rule MF_01225 SAAS SAAS013483

Pathway

Cofactor biosynthesis; molybdopterin biosynthesis. HAMAP-Rule MF_01225 SAAS SAAS013483

Subunit structure

Monomer and homodimer By similarity. HAMAP-Rule MF_01225 SAAS SAAS013483

Sequence similarities

Belongs to the radical SAM superfamily. MoaA family. HAMAP-Rule MF_01225

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region261 – 2633GTP binding By similarity HAMAP-Rule MF_01225

Sites

Metal binding211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01225
Metal binding251Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01225
Metal binding281Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01225
Metal binding2561Iron-sulfur 2 (4Fe-4S-substrate) By similarity HAMAP-Rule MF_01225
Metal binding2591Iron-sulfur 2 (4Fe-4S-substrate) By similarity HAMAP-Rule MF_01225
Metal binding2731Iron-sulfur 2 (4Fe-4S-substrate) By similarity HAMAP-Rule MF_01225
Binding site141GTP By similarity HAMAP-Rule MF_01225
Binding site271S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01225
Binding site651GTP By similarity HAMAP-Rule MF_01225
Binding site691S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_01225
Binding site961GTP By similarity HAMAP-Rule MF_01225
Binding site1201S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01225
Binding site1571GTP By similarity HAMAP-Rule MF_01225
Binding site1911S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_01225

Sequences

Sequence LengthMass (Da)Tools
D7CIA7 [UniParc].

Last modified August 10, 2010. Version 1.
Checksum: 7E89CB1294B6F5FD

FASTA32935,949
        10         20         30         40         50         60 
MLIDTYGRIA TDLRVSLTDR CNLRCTYCMP EEGLQWLAKP DLLTDDEIVR LIRIAVTDLG 

        70         80         90        100        110        120 
VTEVRFTGGE PLLRPGLVGI VERCAALDPR PQMSLTTNGI GLERTAAALR AAGLDRVNVS 

       130        140        150        160        170        180 
LDTLRPEVFQ TMTRRKRHAD VLRGLEAAGA AGLTPVKVNA VLMPGLNDDE APDLLAWAIA 

       190        200        210        220        230        240 
HQYELRFIEQ MPLDAQHGWK RDGMITAGDI LASLRTRFEL TPEGDEARGS APAERWLVDG 

       250        260        270        280        290        300 
GPARVGVIAS VTRPFCRACD RTRLTADGQV RTCLFAREES DLRGALRSGA PDAEIARLWR 

       310        320 
LAMWGKKAGS GLDDPSFLQP DRPMSAIGG

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References

[1]"Genome sequence of the milbemycin-producing bacterium Streptomyces bingchenggensis."
Wang X.J., Yan Y.J., Zhang B., An J., Wang J.J., Tian J., Jiang L., Chen Y.H., Huang S.X., Yin M., Zhang J., Gao A.L., Liu C.X., Zhu Z.X., Xiang W.S.
J. Bacteriol. 192:4526-4527(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BCW-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002047 Genomic DNA. Translation: ADI11285.1.
RefSeqYP_004966416.1. NC_016582.1.

3D structure databases

ProteinModelPortalD7CIA7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADI11285; ADI11285; SBI_08167.
GeneID11606628.
KEGGsbh:SBI_08167.
PATRIC43274506. VBIStrBin158249_8402.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000228681.
KOK03639.
OMAKNIMYLV.

Enzyme and pathway databases

UniPathwayUPA00344.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01225_B. MoaA_B.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR02666. moaA. 1 hit.
PROSITEPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD7CIA7_STRBB
AccessionPrimary (citable) accession number: D7CIA7
Entry history
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info