Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Streptomyces bingchenggensis (strain BCW-1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei256 – 2561ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSBIN749414:GHKA-3537-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:gltXUniRule annotationImported
Ordered Locus Names:SBI_03515Imported
OrganismiStreptomyces bingchenggensis (strain BCW-1)Imported
Taxonomic identifieri749414 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
ProteomesiUP000000377: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD7CCB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi7 – 1711"HIGH" regionUniRule annotationAdd
BLAST
Motifi253 – 2575"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

D7CCB7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVRFCPSPT GNPHVGLVRT ALFNWAFARH HGGTLVFRIE DTDAARDSEE
60 70 80 90 100
SYQQLLDAMR WLGLDWDEGP EIGGPHAPYR QSQRMDLYRD VAERLQESGH
110 120 130 140 150
AYRCYCTAEE LEERREEARK AGRPSGYDGK CRTLTAEQRA AYEAEGRSSI
160 170 180 190 200
VRFRMPDEPI TFTDLVRGEL TFTPENVTDY GIVRANGAPL YTLVNPVDDA
210 220 230 240 250
LMEITHVLRG EDLLSSTPRQ IALYRALAEI GVGGGTVPAF GHLPYVMGEG
260 270 280 290 300
NKKLSKRDPQ ASLNLYRERG FLPEGLLNYL SLLGWSLAPD RDVFSMDELV
310 320 330 340 350
AAFDIAQVNA NPARFDLKKA EAINADHIRQ LDVKAFIEAC GPWLKAPHAP
360 370 380 390 400
WAPEAFDSAA FEALAPLAQT RLTVLSDITA NVDFLFLDEP VEDEASWTKA
410 420 430 440 450
MKPGADALLA SVRTRLAEAE WDAETLKAAV LAAGEEHGLK LGKAQAPVRV
460 470 480
AVTGRTVGLP LFESLEVLGR ERTLARVDAA LAKLTA
Length:486
Mass (Da):53,642
Last modified:August 10, 2010 - v1
Checksum:i531FEC8977AD4A47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002047 Genomic DNA. Translation: ADI06636.1.
RefSeqiYP_004961767.1. NC_016582.1.

Genome annotation databases

EnsemblBacteriaiADI06636; ADI06636; SBI_03515.
GeneIDi11613976.
KEGGisbh:SBI_03515.
PATRICi43264892. VBIStrBin158249_3647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002047 Genomic DNA. Translation: ADI06636.1.
RefSeqiYP_004961767.1. NC_016582.1.

3D structure databases

ProteinModelPortaliD7CCB7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADI06636; ADI06636; SBI_03515.
GeneIDi11613976.
KEGGisbh:SBI_03515.
PATRICi43264892. VBIStrBin158249_3647.

Phylogenomic databases

HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.

Enzyme and pathway databases

BioCyciSBIN749414:GHKA-3537-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the milbemycin-producing bacterium Streptomyces bingchenggensis."
    Wang X.J., Yan Y.J., Zhang B., An J., Wang J.J., Tian J., Jiang L., Chen Y.H., Huang S.X., Yin M., Zhang J., Gao A.L., Liu C.X., Zhu Z.X., Xiang W.S.
    J. Bacteriol. 192:4526-4527(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BCW-1Imported.

Entry informationi

Entry nameiD7CCB7_STRBB
AccessioniPrimary (citable) accession number: D7CCB7
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: February 4, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.