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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Streptomyces bingchenggensis (strain BCW-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 4511D-inositol 3-phosphateUniRule annotation
Binding sitei59 – 591UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei114 – 11411D-inositol 3-phosphateUniRule annotation
Binding sitei147 – 14711D-inositol 3-phosphateUniRule annotation
Binding sitei171 – 17111D-inositol 3-phosphateUniRule annotation
Binding sitei191 – 19111D-inositol 3-phosphateUniRule annotation
Binding sitei266 – 2661UDP-GlcNAcUniRule annotation
Binding sitei271 – 2711UDP-GlcNAcUniRule annotation
Metal bindingi341 – 3411Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi342 – 3421Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi344 – 3441Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei354 – 3541UDP-GlcNAcUniRule annotation
Binding sitei362 – 3621UDP-GlcNAcUniRule annotation
Metal bindingi368 – 3681MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSBIN749414:GHKA-5039-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:SBI_05009
OrganismiStreptomyces bingchenggensis (strain BCW-1)
Taxonomic identifieri749414 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces
Proteomesi
  • UP000000377 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455D-inositol 3-phosphate glycosyltransferasePRO_0000400161Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi749414.SBI_05009.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 522UDP-GlcNAc bindingUniRule annotation
Regioni56 – 6161D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

D7C367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQHVSRLGG LRGRSHGHGA FGGPYHQRLR RVGRRPRRIA MLSVHTSPLH
60 70 80 90 100
QPGTGDAGGM NVYIVELAKR LAAIDIEVEI FTRATTGTLP PTVELAPGVL
110 120 130 140 150
VRHVDAGPYE GLAKEDLPAQ LCAFTHGVMG AWAGHRPGYY DLVHSHYWLS
160 170 180 190 200
GHVGWLAAER WRVPLVHAMH TMAKVKNAAL AEGDTPEPAA RVIGEEQIVG
210 220 230 240 250
AADRLIANTE EEADELVRHY GADPELVAVV HPGVNLERFR PADGRAAARA
260 270 280 290 300
RLGLPPDALI PLFAGRIQPL KAPDILLHAV AHLLDEDPRL RERIVVPVVG
310 320 330 340 350
GPSGSGLAKP ERLHKLAARL GVSDVIRFRP PCTQDELADW YRAASVLVMP
360 370 380 390 400
SYNESFGLVA IEAQACGTPV IAAEVGGLPV AVRDGHSGIL VPGHNPADYA
410 420 430 440 450
RELHRLSADP GLLKRLGSGA AHHAERFGWD KAAAATADVY AEAMRGYRRL

RSVHG
Length:455
Mass (Da):48,982
Last modified:August 10, 2010 - v1
Checksum:i35F7C0A02E619BD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002047 Genomic DNA. Translation: ADI08129.1.
RefSeqiWP_014177596.1. NC_016582.1.

Genome annotation databases

EnsemblBacteriaiADI08129; ADI08129; SBI_05009.
KEGGisbh:SBI_05009.
PATRICi43267974. VBIStrBin158249_5180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002047 Genomic DNA. Translation: ADI08129.1.
RefSeqiWP_014177596.1. NC_016582.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi749414.SBI_05009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADI08129; ADI08129; SBI_05009.
KEGGisbh:SBI_05009.
PATRICi43267974. VBIStrBin158249_5180.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Enzyme and pathway databases

BioCyciSBIN749414:GHKA-5039-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the milbemycin-producing bacterium Streptomyces bingchenggensis."
    Wang X.J., Yan Y.J., Zhang B., An J., Wang J.J., Tian J., Jiang L., Chen Y.H., Huang S.X., Yin M., Zhang J., Gao A.L., Liu C.X., Zhu Z.X., Xiang W.S.
    J. Bacteriol. 192:4526-4527(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BCW-1.
  2. "A catalog of reference genomes from the human microbiome."
    Nelson K.E., Weinstock G.M., Highlander S.K., Worley K.C., Creasy H.H., Wortman J.R., Rusch D.B., Mitreva M., Sodergren E., Chinwalla A.T., Feldgarden M., Gevers D., Haas B.J., Madupu R., Ward D.V., Birren B.W., Gibbs R.A., Methe B.
    , Petrosino J.F., Strausberg R.L., Sutton G.G., White O.R., Wilson R.K., Durkin S., Giglio M.G., Gujja S., Howarth C., Kodira C.D., Kyrpides N., Mehta T., Muzny D.M., Pearson M., Pepin K., Pati A., Qin X., Yandava C., Zeng Q., Zhang L., Berlin A.M., Chen L., Hepburn T.A., Johnson J., McCorrison J., Miller J., Minx P., Nusbaum C., Russ C., Sykes S.M., Tomlinson C.M., Young S., Warren W.C., Badger J., Crabtree J., Markowitz V.M., Orvis J., Cree A., Ferriera S., Fulton L.L., Fulton R.S., Gillis M., Hemphill L.D., Joshi V., Kovar C., Torralba M., Wetterstrand K.A., Abouellleil A., Wollam A.M., Buhay C.J., Ding Y., Dugan S., FitzGerald M.G., Holder M., Hostetler J., Clifton S.W., Allen-Vercoe E., Earl A.M., Farmer C.N., Liolios K., Surette M.G., Xu Q., Pohl C., Wilczek-Boney K., Zhu D.
    Science 328:994-999(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiMSHA_STRBB
AccessioniPrimary (citable) accession number: D7C367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: August 10, 2010
Last modified: May 11, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.