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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Nocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / CIP 107115 / JCM 7437 / KCTC 9190 / NBRC 14626 / NCTC 10488 / NRRL B-5397 / IMRU 509) (Actinomadura dassonvillei)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotationSAAS annotation

Catalytic activityi

ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotationSAAS annotation
[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseImported, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:Ndas_0931Imported
OrganismiNocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / CIP 107115 / JCM 7437 / KCTC 9190 / NBRC 14626 / NCTC 10488 / NRRL B-5397 / IMRU 509) (Actinomadura dassonvillei)Imported
Taxonomic identifieri446468 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptosporangialesNocardiopsaceaeNocardiopsis
Proteomesi
  • UP000002219 Componenti: Chromosome 1

Interactioni

Protein-protein interaction databases

STRINGi446468.Ndas_0931.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini85 – 346GlnEInterPro annotationAdd BLAST262
Domaini371 – 515GlnD_UR_UTaseInterPro annotationAdd BLAST145
Domaini620 – 855GlnEInterPro annotationAdd BLAST236
Domaini879 – 1037GlnD_UR_UTaseInterPro annotationAdd BLAST159

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 519Adenylyl removaseUniRule annotationAdd BLAST519
Regioni527 – 1043Adenylyl transferaseUniRule annotationAdd BLAST517

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000243040.
KOiK00982.
OMAiFTVQLLQ.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

D7B0G8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDGTTNMTA GALARRGFSD STRALRLVEE AGLDARADTE IIGSLAAAPD
60 70 80 90 100
PDQALLGLIR VLESDPDPAD LLDALREDRE LRARLCRVLG TSTALADHLV
110 120 130 140 150
RHPGDWRELR GADAARSPGP EEVRRGLLHI VGADPHSPAP TADPAVVGDG
160 170 180 190 200
SSEDLRHVLR VAYRRRVLRL AGRDLTGLCT VDEVAGELAD LAASLLDASL
210 220 230 240 250
AVARAEHPED AALCRLAVIG MGKCGGRELN YVSDVDVVFV AEPAVAGDAD
260 270 280 290 300
EDAGAGPGSG EPVDEQAAMR AATRLATAMM RIPAETDAEG ALWEVDPALR
310 320 330 340 350
PEGRNGPLVR PLSGHRAYYE RWAKTWEFQA LLKARPIAGD AELGRAYMDL
360 370 380 390 400
ISPMVWEAAG RKDFVEDVQA MRRRVVAHIP AGEAERELKL GPGGLRDIEF
410 420 430 440 450
SVQLLQLVHG RTDERLRSGN TLEALAALSE HGYVGRGDAA GLAEAYRFLR
460 470 480 490 500
RLEHLLQLER LRRTHLMPDP AAEEGPDQLR RLGRALGMTA NPVRELTEAR
510 520 530 540 550
RRVSSEVRRL HEKLFYRPLL NAVARLPGEE ARLSPEQARA RLEALGFVDP
560 570 580 590 600
GGALRHLESL TSGVSRRAAI QRTLLPVMLG WFSDAPDPDA GLLGFRQVSE
610 620 630 640 650
ALGTTPWYLR LLRDDVRVAE RMAWLLGTSR YVTELLLRAP EAVAMFDDDG
660 670 680 690 700
DLVRRDPKVL AGEAEAARRR YDTAEESVSA VRALRRRELL RTAAADLLEV
710 720 730 740 750
ASVQDVGAAL TDIAAVTIEA ALRLAQNRVV AASGDEPLTR VCVVAMGRFG
760 770 780 790 800
GGELTYASDA DVMYVHDPLP GVDTGAATKQ ALAVVRELAR LLEMPAAEPP
810 820 830 840 850
LKVDTDLRPE GRSGPVVRTL ESYAAYYGRW SQVWESQALL RAKPVAGDPQ
860 870 880 890 900
LREAFTGLID PIRYPEGGID SSAVLEIRKL KARMEAERLP RGADPTLHTK
910 920 930 940 950
LGRGGLSDVE WVAQLIQLRH AHEYSDLRTT GTLEALEAAV AHGLLDAEDG
960 970 980 990 1000
EVLSHAWRLA SRVRGMVMLV RGRGGDSLPS DLRVRAALAG AMGCPGGDGE
1010 1020 1030 1040
DGEDGAGEGA ESVREGPAEQ LTETYLRATR RARAVMERVF YDD
Length:1,043
Mass (Da):113,093
Last modified:August 10, 2010 - v1
Checksum:i2BB9F10F9EA7D676
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002040 Genomic DNA. Translation: ADH66375.1.

Genome annotation databases

EnsemblBacteriaiADH66375; ADH66375; Ndas_0931.
KEGGinda:Ndas_0931.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiD7B0G8_NOCDD
AccessioniPrimary (citable) accession number: D7B0G8
Entry historyiIntegrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: June 7, 2017
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported