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Protein

Glutamate-ammonia-ligase adenylyltransferase

Gene

glnE

Organism
Nocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / IMRU 509 / JCM 7437 / NCTC 10488) (Actinomadura dassonvillei)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Adenylation and deadenylation of glutamate--ammonia ligase.UniRule annotationSAAS annotation

Catalytic activityi

ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)].UniRule annotationSAAS annotation

GO - Molecular functioni

  1. [glutamate-ammonia-ligase] adenylyltransferase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. ligase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseImported, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciNDAS446468:GHUM-950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-ammonia-ligase adenylyltransferaseUniRule annotationSAAS annotation (EC:2.7.7.42UniRule annotationSAAS annotation)
Alternative name(s):
Glutamine-synthase adenylyltransferaseUniRule annotation
[Glutamate--ammonia-ligase] adenylyltransferaseUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:Ndas_0931Imported
OrganismiNocardiopsis dassonvillei (strain ATCC 23218 / DSM 43111 / IMRU 509 / JCM 7437 / NCTC 10488) (Actinomadura dassonvillei)Imported
Taxonomic identifieri446468 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeNocardiopsaceaeNocardiopsis
ProteomesiUP000002219: Chromosome 1

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni160 – 374215GlnE 1UniRule annotationAdd
BLAST
Regioni673 – 881209GlnE 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000243040.
KOiK00982.
OMAiEQYYQDQ.

Family and domain databases

HAMAPiMF_00802. GlnE.
InterProiIPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D7B0G8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDGTTNMTA GALARRGFSD STRALRLVEE AGLDARADTE IIGSLAAAPD
60 70 80 90 100
PDQALLGLIR VLESDPDPAD LLDALREDRE LRARLCRVLG TSTALADHLV
110 120 130 140 150
RHPGDWRELR GADAARSPGP EEVRRGLLHI VGADPHSPAP TADPAVVGDG
160 170 180 190 200
SSEDLRHVLR VAYRRRVLRL AGRDLTGLCT VDEVAGELAD LAASLLDASL
210 220 230 240 250
AVARAEHPED AALCRLAVIG MGKCGGRELN YVSDVDVVFV AEPAVAGDAD
260 270 280 290 300
EDAGAGPGSG EPVDEQAAMR AATRLATAMM RIPAETDAEG ALWEVDPALR
310 320 330 340 350
PEGRNGPLVR PLSGHRAYYE RWAKTWEFQA LLKARPIAGD AELGRAYMDL
360 370 380 390 400
ISPMVWEAAG RKDFVEDVQA MRRRVVAHIP AGEAERELKL GPGGLRDIEF
410 420 430 440 450
SVQLLQLVHG RTDERLRSGN TLEALAALSE HGYVGRGDAA GLAEAYRFLR
460 470 480 490 500
RLEHLLQLER LRRTHLMPDP AAEEGPDQLR RLGRALGMTA NPVRELTEAR
510 520 530 540 550
RRVSSEVRRL HEKLFYRPLL NAVARLPGEE ARLSPEQARA RLEALGFVDP
560 570 580 590 600
GGALRHLESL TSGVSRRAAI QRTLLPVMLG WFSDAPDPDA GLLGFRQVSE
610 620 630 640 650
ALGTTPWYLR LLRDDVRVAE RMAWLLGTSR YVTELLLRAP EAVAMFDDDG
660 670 680 690 700
DLVRRDPKVL AGEAEAARRR YDTAEESVSA VRALRRRELL RTAAADLLEV
710 720 730 740 750
ASVQDVGAAL TDIAAVTIEA ALRLAQNRVV AASGDEPLTR VCVVAMGRFG
760 770 780 790 800
GGELTYASDA DVMYVHDPLP GVDTGAATKQ ALAVVRELAR LLEMPAAEPP
810 820 830 840 850
LKVDTDLRPE GRSGPVVRTL ESYAAYYGRW SQVWESQALL RAKPVAGDPQ
860 870 880 890 900
LREAFTGLID PIRYPEGGID SSAVLEIRKL KARMEAERLP RGADPTLHTK
910 920 930 940 950
LGRGGLSDVE WVAQLIQLRH AHEYSDLRTT GTLEALEAAV AHGLLDAEDG
960 970 980 990 1000
EVLSHAWRLA SRVRGMVMLV RGRGGDSLPS DLRVRAALAG AMGCPGGDGE
1010 1020 1030 1040
DGEDGAGEGA ESVREGPAEQ LTETYLRATR RARAVMERVF YDD
Length:1,043
Mass (Da):113,093
Last modified:August 10, 2010 - v1
Checksum:i2BB9F10F9EA7D676
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002040 Genomic DNA. Translation: ADH66375.1.
RefSeqiYP_003678881.1. NC_014210.1.

Genome annotation databases

EnsemblBacteriaiADH66375; ADH66375; Ndas_0931.
GeneIDi9244776.
KEGGinda:Ndas_0931.
PATRICi38211682. VBINocDas52663_0970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002040 Genomic DNA. Translation: ADH66375.1.
RefSeqiYP_003678881.1. NC_014210.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADH66375; ADH66375; Ndas_0931.
GeneIDi9244776.
KEGGinda:Ndas_0931.
PATRICi38211682. VBINocDas52663_0970.

Phylogenomic databases

HOGENOMiHOG000243040.
KOiK00982.
OMAiEQYYQDQ.

Enzyme and pathway databases

BioCyciNDAS446468:GHUM-950-MONOMER.

Family and domain databases

HAMAPiMF_00802. GlnE.
InterProiIPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23218 / DSM 43111 / IMRU 509 / JCM 7437 / NCTC 10488Imported.

Entry informationi

Entry nameiD7B0G8_NOCDD
AccessioniPrimary (citable) accession number: D7B0G8
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: March 4, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.