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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Thermoanaerobacter mathranii subsp. mathranii (strain DSM 11426 / CIP 108742 / A3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotationSAAS annotation

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (Tmath_1227)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation1
Binding sitei35NADPUniRule annotation1
Active sitei141Proton donor/acceptorUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Active sitei145Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13NAD(P)UniRule annotation6
Nucleotide bindingi84 – 86NAD(P)UniRule annotation3
Nucleotide bindingi108 – 111NAD(P)UniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation
LigandNADUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotationSAAS annotation (EC:1.17.1.8UniRule annotationSAAS annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:Tmath_0814Imported
OrganismiThermoanaerobacter mathranii subsp. mathranii (strain DSM 11426 / CIP 108742 / A3)Imported
Taxonomic identifieri583358 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
Proteomesi
  • UP000002064 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD7AU45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 111DapB_NInterPro annotationAdd BLAST110
Domaini114 – 247DapB_CInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 152Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapB family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000227155.
KOiK00215.
OMAiEHEVIFA.

Family and domain databases

HAMAPiMF_00102. DapB. 1 hit.
InterProiView protein in InterPro
IPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR023940. DHDPR_bac.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiView protein in Pfam
PF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
PIRSFiPIRSF000161. DHPR. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiView protein in PROSITE
PS01298. DAPB. 1 hit.

Sequencei

Sequence statusi: Complete.

D7AU45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRIIIHGCN GKMGKVVAKL AAENPDFKIV AGVDKNLSPL EFPVYSDLKD
60 70 80 90 100
VKEEADVVID FSYHEAVPNL VKAALQKKLP VVIATTGLSE EELNVIKDAS
110 120 130 140 150
KEIPIFKSAN MSLGVNILIN LVKEAAKILQ QDFDIEIIEK HHNMKKDAPS
160 170 180 190 200
GTALMIADSI NQVLNDKKEY VYGRHTKTEQ RKTNELGIHA VRGGTIVGEH
210 220 230 240 250
QVIFAGHDEV ITISHSAQSR EIFGYGALHA AKFIIGQKPG LYSMEDLVKK

G
Length:251
Mass (Da):27,590
Last modified:August 10, 2010 - v1
Checksum:i98864B69DD71890F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002032 Genomic DNA. Translation: ADH60552.1.
RefSeqiWP_013150079.1. NC_014209.1.

Genome annotation databases

EnsemblBacteriaiADH60552; ADH60552; Tmath_0814.
KEGGitmt:Tmath_0814.

Similar proteinsi

Entry informationi

Entry nameiD7AU45_THEM3
AccessioniPrimary (citable) accession number: D7AU45
Entry historyiIntegrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: October 25, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.UniRule annotation

Keywords - Technical termi

Complete proteomeImported