Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent 6-phosphofructokinase

Gene

pfk-2

Organism
Geobacter sulfurreducens (strain DL-1 / KN400)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
Active sitei127 – 1271Proton acceptorUniRule annotation
Binding sitei154 – 1541Allosteric activator ADPUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Binding sitei243 – 2431Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATPUniRule annotation
Nucleotide bindingi102 – 1054ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSUL663917:GLDN-2052-MONOMER.
UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfk-2Imported
Synonyms:pfkAUniRule annotation
Ordered Locus Names:KN400_2090Imported
OrganismiGeobacter sulfurreducens (strain DL-1 / KN400)Imported
Taxonomic identifieri663917 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000000389 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

SMRiD7AL48. Positions 1-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni125 – 1273Substrate bindingUniRule annotation
Regioni169 – 1713Substrate bindingUniRule annotation
Regioni185 – 1873Allosteric activator ADP bindingUniRule annotation
Regioni249 – 2524Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D7AL48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIGILTSG GDCSGMNAAI RAAVRTAIRM NIEVVGFRKG YLGLMKGDAI
60 70 80 90 100
PLDTKAVSGI LHRGGTFLQS ARSPEFKTPE GQRTALNNLK ALGVEGMVVM
110 120 130 140 150
GGDGSLTGAL ALNRLGLPVV GIPASIDNDI PFTDMALGVD TALNNIIYAV
160 170 180 190 200
DCIKDTASSH ARAFVIEVMG RHSGYLASIS AIATGAEYAL VPEREYDLAE
210 220 230 240 250
ICQQLRARYE EGRDNAIIIL AEGAGHGHEI ANSIKDAIGF ETRVTVLGHY
260 270 280 290 300
QRGGAPTVFD RLLASRLGKK SVELLVTGTW GVMVGLSCNA ILATPLEDVI
310
KGEKRPQDEV LRLAEVLGV
Length:319
Mass (Da):33,753
Last modified:August 10, 2010 - v1
Checksum:i92C4F19B593FD636
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002031 Genomic DNA. Translation: ADI84902.1.
RefSeqiWP_010942710.1. NC_017454.1.
YP_006891055.1. NC_017454.1.

Genome annotation databases

EnsemblBacteriaiADI84902; ADI84902; KN400_2090.
KEGGigsk:KN400_2090.
PATRICi43028244. VBIGeoSul143269_2090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002031 Genomic DNA. Translation: ADI84902.1.
RefSeqiWP_010942710.1. NC_017454.1.
YP_006891055.1. NC_017454.1.

3D structure databases

SMRiD7AL48. Positions 1-319.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADI84902; ADI84902; KN400_2090.
KEGGigsk:KN400_2090.
PATRICi43028244. VBIGeoSul143269_2090.

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.
BioCyciGSUL663917:GLDN-2052-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "De Novo assembly of the complete genome of an enhanced electricity-producing variant of Geobacter sulfurreducens using only short reads."
    Nagarajan H., Butler J.E., Klimes A., Qiu Y., Zengler K., Ward J., Young N.D., Methe B.A., Palsson B.O., Lovley D.R., Barrett C.L.
    PLoS ONE 5:E10922-E10922(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DL-1 / KN400Imported.

Entry informationi

Entry nameiD7AL48_GEOSK
AccessioniPrimary (citable) accession number: D7AL48
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: April 29, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.