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D7A5Y5

- D7A5Y5_STAND

UniProt

D7A5Y5 - D7A5Y5_STAND

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Starkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB 9113)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Note: Magnesium.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei310 – 3101Coenzyme AUniRule annotation
Binding sitei334 – 3341Coenzyme AUniRule annotation
Binding sitei499 – 4991ATPUniRule annotation
Binding sitei514 – 5141ATPUniRule annotation
Binding sitei522 – 5221Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei525 – 5251ATPUniRule annotation
Metal bindingi536 – 5361Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi538 – 5381Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi541 – 5411Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei583 – 5831Coenzyme AUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi386 – 3883ATPUniRule annotation
Nucleotide bindingi410 – 4156ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSNOV639283:GCS4-2845-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Snov_2812Imported
OrganismiStarkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB 9113)Imported
Taxonomic identifieri639283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeStarkeya
ProteomesiUP000006633: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei608 – 6081N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD7A5Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni190 – 1934Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229981.
KOiK01895.
OMAiAWIWYRD.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D7A5Y5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDKIYDVPA EWAKRAFLNE HAYRAKYEAS VRDTEGFWAE EGKRIEWFEP
60 70 80 90 100
YTIVKNTSYD PGHVSIKWFE DGVTNVAWNC IDRHLPRLAD QTAIIWEGDD
110 120 130 140 150
PSQSRHITYQ ELHDEVCRFA NVLRNRNVEK GDRVTIYMPM IPEAAYAMLA
160 170 180 190 200
CARLGAIHSV VFGGFSPDSL AGRIRDCGSK VIITADEGLR GGRKVPLKAN
210 220 230 240 250
VDAAIAKVSD VEIDHVIVVK RTGGEVDMKP GRDVWYHQAA DLVTSECPAV
260 270 280 290 300
PMNAEDPLFI LYTSGSTGLP KGVLHTTGGY LVYASMTHQY VFDYHDGDVY
310 320 330 340 350
WCTADIGWVT GHSYIVYGPL ANGATTLMFE GVPNYPSNSR FWEVIDKHKV
360 370 380 390 400
NIFYTAPTAI RALMQAGDEP VKKTSRASLR LLGSVGEPIN PEAWEWYHRV
410 420 430 440 450
VGEDRCPIVD TWWQTETGGI LITPLPGATR LKPGSATQPF FGVIPQVVDA
460 470 480 490 500
EGRVLEGACE GNLVIADSWP GQMRTVYGDH ERFMQTYFAT YPGKYFTGDG
510 520 530 540 550
CRRDSDGYYW ITGRVDDVIN VSGHRMGTAE VESALVAHPK VSEAAVVGYP
560 570 580 590 600
HDIKGQGIYA YVTLMAGAEP TEELRKELVA WVRREIGPIA SPDLIQFAPS
610 620 630 640 650
LPKTRSGKIM RRILRKIAED EFGNLGDTST LADPTVVQDL ISQRQNKKHA

AA
Length:652
Mass (Da):72,499
Last modified:August 10, 2010 - v1
Checksum:i1A1A1258EB1694A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002026 Genomic DNA. Translation: ADH90100.1.
RefSeqiWP_013167604.1. NC_014217.1.
YP_003694719.1. NC_014217.1.

Genome annotation databases

EnsemblBacteriaiADH90100; ADH90100; Snov_2812.
GeneIDi9333895.
KEGGisno:Snov_2812.
PATRICi38258209. VBIStaNov45716_2813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002026 Genomic DNA. Translation: ADH90100.1 .
RefSeqi WP_013167604.1. NC_014217.1.
YP_003694719.1. NC_014217.1.

3D structure databases

ProteinModelPortali D7A5Y5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADH90100 ; ADH90100 ; Snov_2812 .
GeneIDi 9333895.
KEGGi sno:Snov_2812.
PATRICi 38258209. VBIStaNov45716_2813.

Phylogenomic databases

HOGENOMi HOG000229981.
KOi K01895.
OMAi AWIWYRD.

Enzyme and pathway databases

BioCyci SNOV639283:GCS4-2845-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB 9113Imported.

Entry informationi

Entry nameiD7A5Y5_STAND
AccessioniPrimary (citable) accession number: D7A5Y5
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: November 26, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3