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Protein

DNA topoisomerase 4 subunit A

Gene

parC

Organism
Streptococcus pneumoniae serotype A19 (strain TCH8431)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei38 – 381Interaction with DNAUniRule annotation
Sitei74 – 741Interaction with DNAUniRule annotation
Sitei76 – 761Interaction with DNAUniRule annotation
Sitei87 – 871Interaction with DNAUniRule annotation
Sitei93 – 931Interaction with DNAUniRule annotation
Sitei117 – 1171Transition state stabilizerUniRule annotation
Active sitei118 – 1181O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Metal bindingi316 – 3161Magnesium; via carbonyl oxygenCombined sources
Metal bindingi319 – 3191Magnesium; via carbonyl oxygenCombined sources
Metal bindingi322 – 3221Magnesium; via carbonyl oxygenCombined sources

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-HAMAP
  2. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation, MagnesiumCombined sources, Metal-bindingCombined sources

Enzyme and pathway databases

BioCyciSPNE525381:GH2H-1675-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit AUniRule annotation
Gene namesi
Name:parCUniRule annotationImported
Ordered Locus Names:HMPREF0837_11636Imported
OrganismiStreptococcus pneumoniae serotype A19 (strain TCH8431)Imported
Taxonomic identifieri525381 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000009083: Chromosome

Subcellular locationi

Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. extrinsic component of plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membraneUniRule annotation, Membrane

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4I3HX-ray3.70A/B1-488[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000076279.
KOiK02621.
OMAiNVLIEMH.
OrthoDBiEOG661H5V.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_00937. ParC_type2.
InterProiIPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
IPR005741. TopoIV_A_Gpos.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 5 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01061. parC_Gpos. 1 hit.

Sequencei

Sequence statusi: Complete.

D6ZLV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNIQNMSLE DIMGERFGRY SKYIIQDRAL PDIRDGLKPV QRRILYSMNK
60 70 80 90 100
DSNTFDKSYR KSAKSVGNIM GNFHPHGDSS IYDAMVRMSQ NWKNREILVE
110 120 130 140 150
MHGNNGSMDG DPPAAMRYTE ARLSEIAGYL LQDIEKKTVP FAWNFDDTEK
160 170 180 190 200
EPTVLPAAFP NLLVNGSTGI SAGYATDIPP HNLAEVIDAA VYMIDHPTAK
210 220 230 240 250
IDKLMEFLPG PDFPTGAIIQ GRDEIKKAYE TGKGRVVVRS KTEIEKLKGG
260 270 280 290 300
KEQIVITEIP YEINKANLVK KIDDVRVNNK VAGIAEVRDE SDRDGLRIAI
310 320 330 340 350
ELKKDANTEL VLNYLFKYTD LQINYNFNMV AIDNFTPRQV GIVPILSSYI
360 370 380 390 400
AHRREVILAR SRFDKEKAEK RLHIVEGLIR VISILDEVIA LIRASENKAD
410 420 430 440 450
AKENLKVSYD FTEEQAEAIV TLQLYRLTNT DVVVLQEEEA ELREKIAMLA
460 470 480 490 500
AIIGDERTMY NLMKKELREV KKKFATPRLS SLEDTAKAIE IDTASLIAEE
510 520 530 540 550
DTYVSVTKAG YIKRTSPRSF AASTLEEIGK RDDDRLIFVQ SAKTTQHLLM
560 570 580 590 600
FTSLGNVIYR PIHELADIRW KDIGEHLSQT ITNFETNEEI LYVEVLDQFD
610 620 630 640 650
DATTYFAVTR LGQIKRVERK EFTPWRTYRS KSVKYAKLKD DTDQIVAVAP
660 670 680 690 700
IKLDDVVLVS QNGYALRFNI EEVPVVGAKA AGVKAMNLKE DDVLQSGFIC
710 720 730 740 750
NTSSFYLLTQ RGSLKRVSIE EILATSRAKR GLQVLRELKN KPHRVFLAGA
760 770 780 790 800
VAEQGFVGDF FSTEVDVNDQ TLLVQSNKGT IYESRLQDLN LSERTSNGSF
810 820
ISDTISDEEV FDAYLQEVVT EDK
Length:823
Mass (Da):93,121
Last modified:August 10, 2010 - v1
Checksum:iC22985D323D63A62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001993 Genomic DNA. Translation: ADI69864.1.
RefSeqiYP_003725078.1. NC_014251.1.

Genome annotation databases

EnsemblBacteriaiADI69864; ADI69864; HMPREF0837_11636.
GeneIDi9344899.
KEGGisnc:HMPREF0837_11636.
PATRICi38264973. VBIStrPne36006_1622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001993 Genomic DNA. Translation: ADI69864.1.
RefSeqiYP_003725078.1. NC_014251.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4I3HX-ray3.70A/B1-488[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADI69864; ADI69864; HMPREF0837_11636.
GeneIDi9344899.
KEGGisnc:HMPREF0837_11636.
PATRICi38264973. VBIStrPne36006_1622.

Phylogenomic databases

HOGENOMiHOG000076279.
KOiK02621.
OMAiNVLIEMH.
OrthoDBiEOG661H5V.

Enzyme and pathway databases

BioCyciSPNE525381:GH2H-1675-MONOMER.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_00937. ParC_type2.
InterProiIPR024946. Arg_repress_C-like.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
IPR005741. TopoIV_A_Gpos.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 5 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01061. parC_Gpos. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A catalog of reference genomes from the human microbiome."
    Nelson K.E., Weinstock G.M., Highlander S.K., Worley K.C., Creasy H.H., Wortman J.R., Rusch D.B., Mitreva M., Sodergren E., Chinwalla A.T., Feldgarden M., Gevers D., Haas B.J., Madupu R., Ward D.V., Birren B.W., Gibbs R.A., Methe B.
    , Petrosino J.F., Strausberg R.L., Sutton G.G., White O.R., Wilson R.K., Durkin S., Giglio M.G., Gujja S., Howarth C., Kodira C.D., Kyrpides N., Mehta T., Muzny D.M., Pearson M., Pepin K., Pati A., Qin X., Yandava C., Zeng Q., Zhang L., Berlin A.M., Chen L., Hepburn T.A., Johnson J., McCorrison J., Miller J., Minx P., Nusbaum C., Russ C., Sykes S.M., Tomlinson C.M., Young S., Warren W.C., Badger J., Crabtree J., Markowitz V.M., Orvis J., Cree A., Ferriera S., Fulton L.L., Fulton R.S., Gillis M., Hemphill L.D., Joshi V., Kovar C., Torralba M., Wetterstrand K.A., Abouellleil A., Wollam A.M., Buhay C.J., Ding Y., Dugan S., FitzGerald M.G., Holder M., Hostetler J., Clifton S.W., Allen-Vercoe E., Earl A.M., Farmer C.N., Liolios K., Surette M.G., Xu Q., Pohl C., Wilczek-Boney K., Zhu D.
    Science 328:994-999(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TCH8431Imported.
  2. "Structure of an 'open' clamp type II topoisomerase-DNA complex provides a mechanism for DNA capture and transport."
    Laponogov I., Veselkov D.A., Crevel I.M., Pan X.S., Fisher L.M., Sanderson M.R.
    Nucleic Acids Res. 41:9911-9923(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.70 ANGSTROMS) OF 1-488 IN COMPLEX WITH MAGNESIUM.

Entry informationi

Entry nameiD6ZLV0_STRP0
AccessioniPrimary (citable) accession number: D6ZLV0
Entry historyi
Integrated into UniProtKB/TrEMBL: August 10, 2010
Last sequence update: August 10, 2010
Last modified: January 7, 2015
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.