Pheromone alpha factor receptor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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D6VTK4 (STE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 25. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pheromone alpha factor receptor

Gene names

Name:	STE2
Ordered Locus Names:	YFL026W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the peptide pheromone alpha factor, the mating factor of yeast.
Subcellular location	Membrane; Multi-pass membrane protein.
Miscellaneous	N-glycosylation may be involved in the sorting process for misfolded STE2 protein.
Sequence similarities	Belongs to the G-protein coupled receptor 4 family.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell projection assembly Inferred from mutant phenotype PubMed 12378535. Source: SGD cytogamy Inferred from direct assay PubMed 17369365. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype PubMed 3037311. Source: SGD protein homooligomerization Inferred from physical interaction PubMed 16709573. Source: SGD
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 2839507. Source: SGD
Molecular_function	mating-type alpha-factor pheromone receptor activity Inferred from direct assay PubMed 2161538. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 431

431

Pheromone alpha factor receptor

PRO_0000195068

Regions

Transmembrane

51 – 73

Helical; Potential

Transmembrane

80 – 102

Helical; Potential

Transmembrane

133 – 155

Helical; Potential

Transmembrane

162 – 184

Helical; Potential

Transmembrane

209 – 231

Helical; Potential

Transmembrane

244 – 266

Helical; Potential

Transmembrane

276 – 298

Helical; Potential

Amino acid modifications

Modified residue

310

Phosphoserine Ref.10

Modified residue

329

Phosphothreonine Ref.10

Modified residue

331

Phosphoserine Ref.10

Modified residue

360

Phosphoserine Ref.10

Modified residue

363

Phosphothreonine Ref.10

Modified residue

366

Phosphoserine Ref.9 Ref.10 Ref.11

Modified residue

382

Phosphothreonine Ref.8 Ref.9 Ref.10 Ref.12

Modified residue

384

Phosphothreonine Ref.8

Modified residue

385

Phosphoserine Ref.8 Ref.10

Modified residue

386

Phosphoserine Ref.10 Ref.12

Modified residue

398

Phosphoserine Ref.12

Modified residue

411

Phosphothreonine Ref.8 Ref.10

Modified residue

414

Phosphothreonine Ref.8 Ref.10

Glycosylation

N-linked (GlcNAc...) Ref.7

Glycosylation

N-linked (GlcNAc...) Ref.7

Experimental info

Sequence conflict

269

K → E in BAA09212. Ref.4

Secondary structure

431

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

D6VTK4 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 011F604DC2A6907E
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FASTA

431

47,849

        10         20         30         40         50         60 
MSDAAPSLSN LFYDPTYNPG QSTINYTSIY GNGSTITFDE LQGLVNSTVT QAIMFGVRCG 

        70         80         90        100        110        120 
AAALTLIVMW MTSRSRKTPI FIINQVSLFL IILHSALYFK YLLSNYSSVT YALTGFPQFI 

       130        140        150        160        170        180 
SRGDVHVYGA TNIIQVLLVA SIETSLVFQI KVIFTGDNFK RIGLMLTSIS FTLGIATVTM 

       190        200        210        220        230        240 
YFVSAVKGMI VTYNDVSATQ DKYFNASTIL LASSINFMSF VLVVKLILAI RSRRFLGLKQ 

       250        260        270        280        290        300 
FDSFHILLIM SCQSLLVPSI IFILAYSLKP NQGTDVLTTV ATLLAVLSLP LSSMWATAAN 

       310        320        330        340        350        360 
NASKTNTITS DFTTSTDRFY PGTLSSFQTD SINNDAKSSL RSRLYDLYPR RKETTSDKHS 

       370        380        390        400        410        420 
ERTFVSETAD DIEKNQFYQL PTPTSSKNTR IGPFADASYK EGEVEPVDMY TPDTAADEEA 

       430 
RKFWTEDNNN L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae." Nakayama N., Miyajima A., Arai K. EMBO J. 4:2643-2648(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204660 / DBY746.
[2]	"The yeast alpha-factor receptor: structural properties deduced from the sequence of the STE2 gene." Burkholder A.C., Hartwell L.H. Nucleic Acids Res. 13:8463-8475(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and implication of recombination in phylogeny." Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S., Souciet J.-L. Nucleic Acids Res. 32:2069-2078(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 269. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Mutational analysis of the role of N-glycosylation in alpha-factor receptor function." Mentesana P.E., Konopka J.B. Biochemistry 40:9685-9694(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-25 AND ASN-32.
[8]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-382; THR-384; SER-385; THR-411 AND THR-414, MASS SPECTROMETRY. Strain: 2124.
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND THR-382, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310; THR-329; SER-331; SER-360; THR-363; SER-366; THR-382; SER-385; SER-386; THR-411 AND THR-414, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-382; SER-386 AND SER-398, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03010 Genomic DNA. Translation: CAA26794.1.
M24335 Genomic DNA. Translation: AAA35112.1.
AJ585736 Genomic DNA. Translation: CAE52256.1.
D50617 Genomic DNA. Translation: BAA09212.1.
AY693134 Genomic DNA. Translation: AAT93153.1.
BK006940 Genomic DNA. Translation: DAA12414.2.

PIR

S56228.

RefSeq

NP_116627.2. NM_001179940.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PJD	NMR	-	A	253-269	[»]
2K9P	NMR	-	A	31-110	[»]

ProteinModelPortal

D6VTK4.

SMR

D6VTK4. Positions 31-110.

ModBase

Search...

Protein-protein interaction databases

IntAct

D6VTK4. 1 interaction.

Protein family/group databases

GPCRDB

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFL026W; YFL026W; YFL026W.

GeneID

850518.

KEGG

sce:YFL026W.

Organism-specific databases

SGD

S000001868. STE2.

Phylogenomic databases

K11213.

OMA

FHILLIM.

Gene expression databases

ArrayExpress

D6VTK4.

GermOnline

YFL026W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000366. GPCR_STE2.
[Graphical view]

Pfam

PF02116. STE2. 1 hit.
[Graphical view]

PRINTS

PR00250. GPCRSTE2.

ProtoNet

Search...

Other

EvolutionaryTrace

D6VTK4.

NextBio

966245.

Entry information

Entry name

STE2_YEAST

Accession

Primary (citable) accession number: D6VTK4
Secondary accession number(s): P06842

Q70D74

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents