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D6RGH6 (MCIN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multicilin
Alternative name(s):
Multiciliate differentiation and DNA synthesis-associated cell cycle protein
Protein Idas
Gene names
Name:MCIDAS
Synonyms:IDAS, MCI, MCIN
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription regulator required for multiciliate cell differentiation. Acts by promoting transcription of genes required for multiciliate cell formation. Probably acts in a multiprotein complex By similarity. Plays a role in mitotic cell cycle progression by promoting cell cycle exit. Modulates GMNN activity by reducing its affinity for CDT1. Ref.1 Ref.3

Subunit structure

Heterodimer (via coiled-coil domain) with GMNN (via coiled-coil domain); targets GMNN to the nucleus. Can form homodimers (in vitro, via coiled-coil domain), but these are much less stable than the heterodimer formed with GMNN. Ref.1 Ref.3

Subcellular location

Nucleus. Note: Excluded from the nucleolus. Ref.1

Developmental stage

Probable target of the anaphase promoting complex/cyclosome (APC/C) which regulates its level in the cell during the mitotic cell cycle. Highly expressed during interphase and early mitosis. Expression decreases during anaphase to become undetectable during telophase and cytokinesis. Ref.1

Miscellaneous

Was named Idas in reference to the cousin of the Gemini in ancient Greek mythology (Ref.1).

Sequence similarities

Belongs to the geminin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-3954372,EBI-3954372
GMNNO754967EBI-3954372,EBI-371669

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385Multicilin
PRO_0000411076

Regions

Region1 – 130130Necessary and sufficient for its degradation during the cell cycle
Region131 – 385255Necessary and sufficient for proper nuclear localization
Region173 – 24573Necessary and sufficient for interaction with GMNN and sufficient for homodimerization
Coiled coil179 – 22749 Ref.3

Secondary structure

..... 385
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
D6RGH6 [UniParc].

Last modified July 13, 2010. Version 1.
Checksum: D52A35B926E75723

FASTA38541,720
        10         20         30         40         50         60 
MQACGGGAAG RRAFDSICPN RMLALPGRAL LCKPGKPERK FAPPRKFFPG CTGGSPVSVY 

        70         80         90        100        110        120 
EDPPDAEPTA LPALTTIDLQ DLADCSSLLG SDAPPGGDLA ASQNHSHQTE ADFNLQDFRD 

       130        140        150        160        170        180 
TVDDLISDSS SMMSPTLASG DFPFSPCDIS PFGPCLSPPL DPRALQSPPL RPPDVPPPEQ 

       190        200        210        220        230        240 
YWKEVADQNQ RALGDALVEN NQLHVTLTQK QEEIASLKER NVQLKELASR TRHLASVLDK 

       250        260        270        280        290        300 
LMITQSRDCG AAAEPFLLKA KAKRSLEELV SAAGQDCAEV DAILREISER CDEALQSRDP 

       310        320        330        340        350        360 
KRPRLLPEPA NTDTRPGNLH GAFRGLRTDC SRSALNLSHS ELEEGGSFST RIRSHSTIRT 

       370        380 
LAFPQGNAFT IRTANGGYKF RWVPS 

« Hide

References

« Hide 'large scale' references
[1]"Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression."
Pefani D.E., Dimaki M., Spella M., Karantzelis N., Mitsiki E., Kyrousi C., Symeonidou I.E., Perrakis A., Taraviras S., Lygerou Z.
J. Biol. Chem. 286:23234-23246(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GMNN, HOMODIMERIZATION, SUBUNIT, DEVELOPMENTAL STAGE.
[2]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing."
Caillat C., Pefani E.D., Gillespie P.J., Taraviras S., Blow J.J., Lygerou Z., Perrakis A.
J. Biol. Chem. 288:31624-31634(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.89 ANGSTROMS) OF 173-245 IN COMPLEX WITH GMNN, FUNCTION, COILED-COIL DOMAIN, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FR854393 mRNA. Translation: CCA89438.1.
AC091977 Genomic DNA. No translation available.
RefSeqNP_001177716.1. NM_001190787.1.
UniGeneHs.394578.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BRYX-ray2.89B173-245[»]
ProteinModelPortalD6RGH6.
SMRD6RGH6. Positions 176-244.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActD6RGH6. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000513312; ENSP00000426359; ENSG00000234602.
GeneID345643.
KEGGhsa:345643.
UCSCuc021xyp.1. human.

Organism-specific databases

CTD345643.
GeneCardsGC05M054521.
HGNCHGNC:40050. MCIDAS.
HPAHPA049981.
MIM614086. gene.
neXtProtNX_D6RGH6.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHOG000133078.
KOK10749.
OMAGYKFRWV.

Family and domain databases

InterProIPR022786. Geminin_fam.
[Graphical view]
PANTHERPTHR13372. PTHR13372. 1 hit.
PfamPF07412. Geminin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi345643.
NextBio98889.
PROD6RGH6.
SOURCESearch...

Entry information

Entry nameMCIN_HUMAN
AccessionPrimary (citable) accession number: D6RGH6
Secondary accession number(s): C9JGY3, D6R920, F8KGQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 13, 2010
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM