D6RGH6 (MCIN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multicilin

Alternative name(s):
Multiciliate differentiation and DNA synthesis-associated cell cycle protein
Protein Idas

Gene names

Name:	MCIDAS
Synonyms:	IDAS, MCI, MCIN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription regulator required for multiciliate cell differentiation. Acts by promoting transcription of genes required for multiciliate cell formation. Probably acts in a multiprotein complex By similarity. Plays a role in mitotic cell cycle progression by promoting cell cycle exit. Ref.1
Subunit structure	Homodimer. Interacts with GMNN; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Ref.1
Subcellular location	Nucleus. Note: Excluded from the nucleolus. Ref.1
Developmental stage	Probable target of the anaphase promoting complex/cyclosome (APC/C) which regulates its level in the cell during the mitotic cell cycle. Highly expressed during interphase and early mitosis. Expression decreases during anaphase to become undetectable during telophase and cytokinesis. Ref.1
Miscellaneous	Was named Idas in reference to the cousin of the Gemini in ancient Greek mythology (Ref.1).
Sequence similarities	Belongs to the geminin family.

Ontologies

Keywords
Biological process	Cell cycle Cilium biogenesis/degradation Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cilium assembly Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB regulation of DNA replication Inferred from electronic annotation. Source: InterPro regulation of mitotic cell cycle Inferred from mutant phenotype Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-3954372,EBI-3954372
GMNN	O75496	7	EBI-3954372,EBI-371669

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Multicilin		PRO_0000411076
Regions
	Region	1 – 130	130	Necessary and sufficient for its degradation during the cell cycle
	Region	131 – 385	255	Necessary and sufficient for proper nuclear localization
	Region	173 – 245	73	Necessary and sufficient for interaction with GMNN and sufficient for homodimerization
	Coiled coil	198 – 233	36	Potential

Sequences

	Sequence		Length	Mass (Da)
	D6RGH6 [UniParc]. Last modified July 13, 2010. Version 1. Checksum: D52A35B926E75723	FASTA	385	41,720
	10 20 30 40 50 60 MQACGGGAAG RRAFDSICPN RMLALPGRAL LCKPGKPERK FAPPRKFFPG CTGGSPVSVY 70 80 90 100 110 120 EDPPDAEPTA LPALTTIDLQ DLADCSSLLG SDAPPGGDLA ASQNHSHQTE ADFNLQDFRD 130 140 150 160 170 180 TVDDLISDSS SMMSPTLASG DFPFSPCDIS PFGPCLSPPL DPRALQSPPL RPPDVPPPEQ 190 200 210 220 230 240 YWKEVADQNQ RALGDALVEN NQLHVTLTQK QEEIASLKER NVQLKELASR TRHLASVLDK 250 260 270 280 290 300 LMITQSRDCG AAAEPFLLKA KAKRSLEELV SAAGQDCAEV DAILREISER CDEALQSRDP 310 320 330 340 350 360 KRPRLLPEPA NTDTRPGNLH GAFRGLRTDC SRSALNLSHS ELEEGGSFST RIRSHSTIRT 370 380 LAFPQGNAFT IRTANGGYKF RWVPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression." Pefani D.E., Dimaki M., Spella M., Karantzelis N., Mitsiki E., Kyrousi C., Symeonidou I.E., Perrakis A., Taraviras S., Lygerou Z. J. Biol. Chem. 286:23234-23246(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GMNN, HOMODIMERIZATION, DEVELOPMENTAL STAGE.
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	FR854393 mRNA. Translation: CCA89438.1. AC091977 Genomic DNA. No translation available.
IPI	IPI00748260.
RefSeq	NP_001177716.1. NM_001190787.1.
UniGene	Hs.394578.
3D structure databases
ProteinModelPortal	D6RGH6.
SMR	D6RGH6. Positions 177-235.
ModBase	Search...
Protein-protein interaction databases
IntAct	D6RGH6. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000513312; ENSP00000426359; ENSG00000234602.
GeneID	345643.
KEGG	hsa:345643.
UCSC	uc021xyp.1. human.
Organism-specific databases
CTD	345643.
GeneCards	GC05M054520.
HGNC	HGNC:40050. MCIDAS.
HPA	HPA049981.
MIM	614086. gene.
neXtProt	NX_D6RGH6.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HOG000133078.
KO	K10749.
OMA	GYKFRWV.
Family and domain databases
InterPro	IPR022786. Geminin_fam. [Graphical view]
PANTHER	PTHR13372. PTHR13372. 1 hit.
Pfam	PF07412. Geminin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	345643.
NextBio	98889.
SOURCE	Search...

Entry information

Entry name

MCIN_HUMAN

Accession

Primary (citable) accession number: D6RGH6
Secondary accession number(s): C9JGY3, D6R920, F8KGQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	July 13, 2010
Last modified:	May 1, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.