Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

D5XEG9 (D5XEG9_THEPJ) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:TherJR_1176 EMBL ADG82040.1
OrganismThermincola potens (strain JR) [Complete proteome] [HAMAP] EMBL ADG82040.1
Taxonomic identifier635013 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeThermincola

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain141 – 347207ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding174 – 22956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3141Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3161Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D5XEG9 [UniParc].

Last modified July 13, 2010. Version 1.
Checksum: 536F4F38DB8BBB94

FASTA36540,380
        10         20         30         40         50         60 
MKKNRVAVIF GGKSGEHEVS LASANSVLQA LNKEKYEIIP IKISREGKWY ILSSLNTFDS 

        70         80         90        100        110        120 
NIKCEAAILA DPTGDNLVNL QEKEPGQIFF TRESVDVVLP VLHGTFGEDG TLQGLLELAN 

       130        140        150        160        170        180 
IPYVGCGVAA SSVGMDKAMM KAMFAQHNLP QGKYLTFLRK EWEKDQDPIC DEIETALGYP 

       190        200        210        220        230        240 
VFVKPANLGS SVGISKAANR DELVKAMHLA CLYDRKVVVE ENINGREIEV AVLGNDEPEA 

       250        260        270        280        290        300 
SIAGEIVPCN DFYDYEAKYI SGTSELKIPA PLDDKTMNEV RKLAVKAFKA IDGAGLSRVD 

       310        320        330        340        350        360 
FFIKKDTGEI LINEINTLPG FTEISMYPKL WAATGLPYDK LLDRLIELAI ERHQDKNRNK 


TVYSE 

« Hide

References

[1]"Complete sequence of Thermincola sp. JR."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Mikhailova N., Hazen T.C., Woyke T.
Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JR EMBL ADG82040.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002028 Genomic DNA. Translation: ADG82040.1.
RefSeqYP_003639941.1. NC_014152.1.

3D structure databases

ProteinModelPortalD5XEG9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADG82040; ADG82040; TherJR_1176.
GeneID9148863.
KEGGtjr:TherJR_1176.
PATRIC38273925. VBITheSp141296_1261.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.

Enzyme and pathway databases

BioCycTPOT635013:GHIM-1216-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5XEG9_THEPJ
AccessionPrimary (citable) accession number: D5XEG9
Entry history
Integrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)