D5WZX8 (D5WZX8_THIK1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Hydrogenase (NiFe) small subunit HydA EMBL ADG32406.1
EC=1.12.99.6 EMBL ADG32406.1

Gene names

Ordered Locus Names:

Tint_3077

Organism

Thiomonas intermedia (strain K12) (Thiobacillus intermedius) [Complete proteome] [HAMAP]

Taxonomic identifier

75379 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › unclassified Burkholderiales › Burkholderiales Genera incertae sedis › Thiomonas › Thiomonas intermedia

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor

Binds 1 3Fe-4S cluster By similarity. PIRSR PIRSR000310-1 Binds 2 4Fe-4S clusters By similarity. PIRSR PIRSR000310-1

Ontologies

Keywords
Ligand	3Fe-4S PIRSR PIRSR000310-1 4Fe-4S PIRSR PIRSR000310-1 Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase EMBL ADG32406.1
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	65	1	Iron-sulfur 1 (4Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	160	1	Iron-sulfur 1 (4Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	194	1	Iron-sulfur 1 (4Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	232	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity PIRSR PIRSR000310-1
	Metal binding	235	1	Iron-sulfur 2 (4Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	260	1	Iron-sulfur 2 (4Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	266	1	Iron-sulfur 2 (4Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	275	1	Iron-sulfur 3 (3Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	294	1	Iron-sulfur 3 (3Fe-4S) By similarity PIRSR PIRSR000310-1
	Metal binding	297	1	Iron-sulfur 3 (3Fe-4S) By similarity PIRSR PIRSR000310-1

Sequences

	Sequence		Length	Mass (Da)
	D5WZX8 [UniParc]. Last modified July 13, 2010. Version 1. Checksum: 2FA6E8388DBFA57E	FASTA	370	40,541
	10 20 30 40 50 60 MPVTETFYEV MRRQGISRRS FIKYCSLTAA ALGLGPQYAT EIAYAMENKP RLPVLWLHGL 70 80 90 100 110 120 ECTCCSESFI RSGHPLAKDV ILSMISLDYD DTLMAAAGHQ AEAIVEEVVN KYKGNYILAV 130 140 150 160 170 180 EGNPPLNEDG MYCIIAGQPF VEKLRYAASN AKAIISWGSC ASWGCVQAAK PNPTRATPVH 190 200 210 220 230 240 KVITDKPLIK VPGCPPIAEV MTAVITYMLT FDRMPELDRQ GRPKMFYSQR IHDKCYRRPN 250 260 270 280 290 300 FDAGQFVEAF DDENARKGYC LYKVGCKGPT TYNACSTTRW NDGVSFPIQS GHGCIGCSED 310 320 330 340 350 360 GFWDKGSWYA HLTDIKPFGV EANADDIGAA AAGVVGAAVG VHAVASVVKR LRSSDQPKTK 370 NETEHTGGKE

References

[1]

"Complete sequence of Thiomonas intermedia K12." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Kerfeld C.A., Cannon G.C., Heinhorst S., Woyke T. Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 EMBL ADG32406.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002021 Genomic DNA. Translation: ADG32406.1.
RefSeq	YP_003644736.1. NC_014153.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADG32406; ADG32406; Tint_3077.
GeneID	9153814.
KEGG	tin:Tint_3077.
PATRIC	38296751. VBIThiInt85915_3108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000278822.
KO	K06282.
Family and domain databases
Gene3D	3.40.50.700. 1 hit.
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D5WZX8_THIK1

Accession

Primary (citable) accession number: D5WZX8

Entry history

Integrated into UniProtKB/TrEMBL:	July 13, 2010
Last sequence update:	July 13, 2010
Last modified:	May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)