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D5WD20

- D5WD20_BURSC

UniProt

D5WD20 - D5WD20_BURSC

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Protein
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Gene
miaB, BC1002_2597
Organism
Burkholderia sp. (strain CCGE1002)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity.UniRule annotationSAAS annotations

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotationSAAS annotations

Cofactori

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarityUniRule annotation
Metal bindingi157 – 1571Iron-sulfur (4Fe-4S-S-AdoMet) By similarityUniRule annotation
Metal bindingi161 – 1611Iron-sulfur (4Fe-4S-S-AdoMet) By similarityUniRule annotation
Metal bindingi164 – 1641Iron-sulfur (4Fe-4S-S-AdoMet) By similarityUniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotationsImported

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotations

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotations, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciBSP640511:GJ7J-2649-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:BC1002_2597Imported
OrganismiBurkholderia sp. (strain CCGE1002)Imported
Taxonomic identifieri640511 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000002190: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliD5WD20.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 120118MTTase N-terminal By similarityUniRule annotation
Add
BLAST
Domaini384 – 45168TRAM By similarityUniRule annotation
Add
BLAST

Sequence similaritiesi

Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation
Contains MTTase N-terminal domain.SAAS annotations
Contains TRAM domain.SAAS annotations

Phylogenomic databases

HOGENOMiHOG000224767.
KOiK06168.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5WD20-1 [UniParc]FASTAAdd to Basket

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MTKKVYVKTF GCQMNEYDSD KMVDVLGAAE GLVKTDTPED ADVILFNTCS    50
VREKAQEKVF SDLGRVRELK DANPNLIIGV GGCVASQEGA SIVARAPYVD 100
IVFGPQTLHR LPQMIDERRA SGRAQVDISF PEIEKFDHLP PARVEGPSAF 150
VSIMEGCSKY CSYCVVPYTR GEEVSRPLDD VLTEIAGLAD QGVREVTLLG 200
QNVNAYRGKF GGALTLGSTE IADFATLIEY VADIPGIERI RYTTSHPKEF 250
TQRLIDTYAK VPKLVSHLHL PVQHGSDRIL MAMKRGYTVL EYKSVIRKLR 300
AIRPDLSLST DMIVGFPGET EEDFAKMMAL VEEMKYDTSF SFIYSPRPGT 350
PAANLHDDTP REVKLKRLQH LQATIEENVQ RISDAMVGNV ERILVERPAR 400
KDPNELAGRT ENNRVVNFPA PVASHARLIG QMVDVKIVHA YPHSLRGELV 450
MVRDDSTAAT H 461
Length:461
Mass (Da):51,233
Last modified:July 13, 2010 - v1
Checksum:iDEC344B16A61E009
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002013 Genomic DNA. Translation: ADG16646.1.
RefSeqiWP_013090486.1. NC_014117.1.
YP_003606157.1. NC_014117.1.

Genome annotation databases

EnsemblBacteriaiADG16646; ADG16646; BC1002_2597.
GeneIDi9090309.
KEGGibge:BC1002_2597.
PATRICi37214178. VBIBurSp41388_2663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002013 Genomic DNA. Translation: ADG16646.1 .
RefSeqi WP_013090486.1. NC_014117.1.
YP_003606157.1. NC_014117.1.

3D structure databases

ProteinModelPortali D5WD20.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADG16646 ; ADG16646 ; BC1002_2597 .
GeneIDi 9090309.
KEGGi bge:BC1002_2597.
PATRICi 37214178. VBIBurSp41388_2663.

Phylogenomic databases

HOGENOMi HOG000224767.
KOi K06168.

Enzyme and pathway databases

BioCyci BSP640511:GJ7J-2649-MONOMER.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
HAMAPi MF_01864. tRNA_metthiotr_MiaB.
InterProi IPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view ]
PANTHERi PTHR11918. PTHR11918. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
PROSITEi PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia sp. CCGE1002."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Martinez-Romero E., Hernandez M.A.R., Tiedje J.M., Woyke T.
    Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCGE1002Imported.

Entry informationi

Entry nameiD5WD20_BURSC
AccessioniPrimary (citable) accession number: D5WD20
Entry historyi
Integrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: September 3, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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