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D5WD20 (D5WD20_BURSC) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase HAMAP-Rule MF_01864

EC=2.8.4.3 HAMAP-Rule MF_01864
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864
tRNA-i(6)A37 methylthiotransferase HAMAP-Rule MF_01864
Gene names
Name:miaB HAMAP-Rule MF_01864
Ordered Locus Names:BC1002_2597 EMBL ADG16646.1
OrganismBurkholderia sp. (strain CCGE1002) [Complete proteome] [HAMAP] EMBL ADG16646.1
Taxonomic identifier640511 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864 SAAS SAAS006463

Catalytic activity

N(6)-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine. HAMAP-Rule MF_01864 SAAS SAAS006463

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_01864 SAAS SAAS006463

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864 SAAS SAAS006463

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01864 SAAS SAAS006463.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily. HAMAP-Rule MF_01864

Contains 1 MTTase N-terminal domain. HAMAP-Rule MF_01864

Contains 1 TRAM domain. HAMAP-Rule MF_01864

Contains MTTase N-terminal domain. SAAS SAAS006463

Contains TRAM domain. SAAS SAAS006463

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain3 – 120118MTTase N-terminal By similarity HAMAP-Rule MF_01864
Domain384 – 45168TRAM By similarity HAMAP-Rule MF_01864

Sites

Metal binding121Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01864
Metal binding491Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01864
Metal binding831Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01864
Metal binding1571Iron-sulfur (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01864
Metal binding1611Iron-sulfur (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01864
Metal binding1641Iron-sulfur (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01864

Sequences

Sequence LengthMass (Da)Tools
D5WD20 [UniParc].

Last modified July 13, 2010. Version 1.
Checksum: DEC344B16A61E009

FASTA46151,233
        10         20         30         40         50         60 
MTKKVYVKTF GCQMNEYDSD KMVDVLGAAE GLVKTDTPED ADVILFNTCS VREKAQEKVF 

        70         80         90        100        110        120 
SDLGRVRELK DANPNLIIGV GGCVASQEGA SIVARAPYVD IVFGPQTLHR LPQMIDERRA 

       130        140        150        160        170        180 
SGRAQVDISF PEIEKFDHLP PARVEGPSAF VSIMEGCSKY CSYCVVPYTR GEEVSRPLDD 

       190        200        210        220        230        240 
VLTEIAGLAD QGVREVTLLG QNVNAYRGKF GGALTLGSTE IADFATLIEY VADIPGIERI 

       250        260        270        280        290        300 
RYTTSHPKEF TQRLIDTYAK VPKLVSHLHL PVQHGSDRIL MAMKRGYTVL EYKSVIRKLR 

       310        320        330        340        350        360 
AIRPDLSLST DMIVGFPGET EEDFAKMMAL VEEMKYDTSF SFIYSPRPGT PAANLHDDTP 

       370        380        390        400        410        420 
REVKLKRLQH LQATIEENVQ RISDAMVGNV ERILVERPAR KDPNELAGRT ENNRVVNFPA 

       430        440        450        460 
PVASHARLIG QMVDVKIVHA YPHSLRGELV MVRDDSTAAT H 

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia sp. CCGE1002."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Martinez-Romero E., Hernandez M.A.R., Tiedje J.M., Woyke T.
Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCGE1002 EMBL ADG16646.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002013 Genomic DNA. Translation: ADG16646.1.
RefSeqYP_003606157.1. NC_014117.1.

3D structure databases

ProteinModelPortalD5WD20.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADG16646; ADG16646; BC1002_2597.
GeneID9090309.
KEGGbge:BC1002_2597.
PATRIC37214178. VBIBurSp41388_2663.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000224767.
KOK06168.

Enzyme and pathway databases

BioCycBSP640511:GJ7J-2649-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5WD20_BURSC
AccessionPrimary (citable) accession number: D5WD20
Entry history
Integrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: June 11, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)