ID   D5W5A4_PARAM            Unreviewed;       746 AA.
AC   D5W5A4;
DT   13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT   13-JUL-2010, sequence version 1.
DT   24-JAN-2024, entry version 70.
DE   RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR009407};
DE            EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR009407};
DE   AltName: Full=Oxalosuccinate decarboxylase {ECO:0000256|PIRNR:PIRNR009407};
GN   OrderedLocusNames=BC1002_0850 {ECO:0000313|EMBL:ADG14946.1};
GN   ORFNames=HDG40_004433 {ECO:0000313|EMBL:MBB5426259.1};
OS   Paraburkholderia atlantica.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=2654982 {ECO:0000313|EMBL:ADG14946.1, ECO:0000313|Proteomes:UP000002190};
RN   [1] {ECO:0000313|EMBL:ADG14946.1, ECO:0000313|Proteomes:UP000002190}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCGE1002 {ECO:0000313|EMBL:ADG14946.1,
RC   ECO:0000313|Proteomes:UP000002190};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA   Kyrpides N., Ovchinnikova G., Martinez-Romero E., Hernandez M.A.R.,
RA   Tiedje J.M., Woyke T.;
RT   "Complete sequence of chromosome 1 of Burkholderia sp. CCGE1002.";
RL   Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ADG14946.1, ECO:0000313|Proteomes:UP000002190}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCGE1002 {ECO:0000313|EMBL:ADG14946.1,
RC   ECO:0000313|Proteomes:UP000002190};
RX   PubMed=23209196; DOI=10.1128/JB.01756-12;
RA   Ormeno-Orrillo E., Rogel M.A., Chueire L.M., Tiedje J.M.,
RA   Martinez-Romero E., Hungria M.;
RT   "Genome Sequences of Burkholderia sp. Strains CCGE1002 and H160, Isolated
RT   from Legume Nodules in Mexico and Brazil.";
RL   J. Bacteriol. 194:6927-6927(2012).
RN   [3] {ECO:0000313|EMBL:MBB5426259.1, ECO:0000313|Proteomes:UP000592780}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JPY158 {ECO:0000313|EMBL:MBB5426259.1,
RC   ECO:0000313|Proteomes:UP000592780};
RA   Whitman W.;
RT   "Genomic Encyclopedia of Type Strains, Phase IV (KMG-V): Genome sequencing
RT   to study the core and pangenomes of soil and plant-associated
RT   prokaryotes.";
RL   Submitted (AUG-2020) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC         Evidence={ECO:0000256|PIRNR:PIRNR009407};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000256|PIRSR:PIRSR009407-3};
CC   -!- SIMILARITY: Belongs to the monomeric-type IDH family.
CC       {ECO:0000256|PIRNR:PIRNR009407}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP002013; ADG14946.1; -; Genomic_DNA.
DR   EMBL; JACHDD010000007; MBB5426259.1; -; Genomic_DNA.
DR   RefSeq; WP_013088834.1; NZ_VTVD01000002.1.
DR   AlphaFoldDB; D5W5A4; -.
DR   STRING; 640511.BC1002_0850; -.
DR   KEGG; bge:BC1002_0850; -.
DR   eggNOG; COG2838; Bacteria.
DR   HOGENOM; CLU_025308_1_0_4; -.
DR   Proteomes; UP000002190; Chromosome 1.
DR   Proteomes; UP000592780; Unassembled WGS sequence.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.718.10; Isopropylmalate Dehydrogenase; 1.
DR   InterPro; IPR004436; Isocitrate_DH_NADP_mono.
DR   NCBIfam; TIGR00178; monomer_idh; 1.
DR   PANTHER; PTHR36999; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR   PANTHER; PTHR36999:SF1; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR   Pfam; PF03971; IDH; 1.
DR   PIRSF; PIRSF009407; IDH_monmr; 1.
DR   SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
PE   3: Inferred from homology;
KW   Glyoxylate bypass {ECO:0000256|PIRNR:PIRNR009407};
KW   Magnesium {ECO:0000256|PIRSR:PIRSR009407-3};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR009407-3};
KW   NADP {ECO:0000256|PIRNR:PIRNR009407};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR009407,
KW   ECO:0000313|EMBL:ADG14946.1};
KW   Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR009407}.
FT   BINDING         134..141
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT   BINDING         147
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT   BINDING         352
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT   BINDING         550
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT   BINDING         551
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT   BINDING         555
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT   SITE            257
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
FT   SITE            422
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
SQ   SEQUENCE   746 AA;  82635 MW;  05503DBB412A69BA CRC64;
     MTTQQPTIIY TLTDEAPLLA TAAFLPIIRT FTRPAGIEVE TSDISVAGRI LGEFPEFLTE
     AQRVPDNLAE LGRLTSLPDT NIIKLPNISA SVPQLTSAIK ELQAKGYKVP DYPEDPKTDA
     EKAIRQRYSK CLGSAVNPVL REGNSDRRAP AAVKNYARKH PHSMTEWSMA SRTHVATMRH
     GDFYHGEKSM TLDRARDVKM ELVTKNGKTI VLKPKVALED GEIIDSMFMS KKALLAFYED
     QMEDARKTGM MLSLHVKATM MKISHPIVFG HAVKVFYKDA FAKHGKLFEE LGVNVNNGLV
     NLYEKIEALP ESQREEVIRD LHACHEHRPE LAMVDSAKGI SNLHAPNDVI VDASMPAMIR
     AGGKMWGADG RPKDTKAVIP ESTFARIYQE IINFCKTNGA FDPVTMGTVP NVGLMAQKAE
     EYGSHDKTFE VPEDGEARIV DLASGEVLLT QNVEQGDIWR MCEVKDAAIR DWVKLAVTRV
     RNSRTPAVFW LDPYRPHEAE LIKKVEAYLK DYDTTGLDIQ IMSQVRAMRY TLERVVRGLD
     TISVTGNILR DYLTDLFPIM ELGTSAKMLS IVPLMAGGGM YETGAGGSAP KHVKQLVEEN
     HLRWDSLGEF LALAVSLEEL GIKTGNNRAK ILAKTLDTAT GKLLDNNRSP SPKTGELDNR
     GSQFYLAMYW AQELAAQTDD AALAAQFAPL ATQLTANEQV IVAELASVQG APADIGGYYK
     PDFNKLEEVM RPSRTFNEAL KAVRVE
//