D5VUB0 (RIBL_METIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FAD synthase
EC=2.7.7.2

Alternative name(s):
FMN adenylyltransferase
Flavin adenine dinucleotide synthase

Gene names

Name:	ribL
Ordered Locus Names:	Metin_0050

Organism

Methanocaldococcus infernus (strain DSM 11812 / JCM 15783 / ME) [Complete proteome] [HAMAP]

Taxonomic identifier

573063 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus infernus

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme By similarity. HAMAP-Rule MF_02115
Catalytic activity	ATP + FMN = diphosphate + FAD. HAMAP-Rule MF_02115
Cofactor	Divalent metal cations By similarity.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. HAMAP-Rule MF_02115
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the archaeal FAD synthase family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: HAMAP FMN metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP FMN adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	FAD synthase HAMAP-Rule MF_02115		PRO_0000406248
Regions
	Nucleotide binding	9 – 10	2	ATP By similarity
	Nucleotide binding	14 – 17	4	ATP By similarity
	Nucleotide binding	92 – 95	4	ATP By similarity
Sites
	Binding site	122	1	ATP; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	D5VUB0 [UniParc]. Last modified July 13, 2010. Version 1. Checksum: 0C7C2D7DBEB02383	FASTA	145	16,745
	10 20 30 40 50 60 MKRVVAAGTF DILHPGHYEF LKFAKSLGDE LIVIVARDKT VEKIKGRKPI IPEEQRRAMV 70 80 90 100 110 120 EALKPVDKAI LGSLNNKLEP IIELKPDIIV LGPDQRTFDE EELKRELKKY DLSPKIVRFN 130 140 KYIKCPFHSS YDIVKEILKR YGGKE

References

[1]

"Complete sequence of Methanocaldococcus infernus ME." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Woyke T. Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11812 / JCM 15783 / ME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002009 Genomic DNA. Translation: ADG12722.1.
RefSeq	YP_003615686.1. NC_014122.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADG12722; ADG12722; Metin_0050.
GeneID	9131049.
KEGG	mif:Metin_0050.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000284153.
KO	K14656.
OMA	TFDVFHV.
Enzyme and pathway databases
BioCyc	MINF573063:GHL3-51-MONOMER.
UniPathway	UPA00277; UER00407.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_02115. FAD_synth_arch.
InterPro	IPR004821. Cyt_trans-like. IPR024902. FAD_synth_RibL. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF01467. CTP_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBL_METIM

Accession

Primary (citable) accession number: D5VUB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	July 13, 2010
Last modified:	May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program