D5VRG1 (D5VRG1_METIM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420
EC=6.3.5.3 HAMAP-Rule MF_00420

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II HAMAP-Rule MF_00420

Gene names

Name:	purL HAMAP-Rule MF_00420
Ordered Locus Names:	Metin_0494

Organism

Methanocaldococcus infernus (strain DSM 11812 / JCM 15783 / ME) [Complete proteome] [HAMAP]

Taxonomic identifier

573063 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus › Methanocaldococcus infernus

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity. HAMAP-Rule MF_00420
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00420.
Sequence similarities	Belongs to the FGAMS family. HAMAP-Rule MF_00420

Ontologies

Keywords
Biological process	Purine biosynthesis HAMAP-Rule MF_00420
Cellular component	Cytoplasm HAMAP-Rule MF_00420
Ligand	ATP-binding HAMAP-Rule MF_00420 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00420 EMBL ADG13164.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	94 – 105	12	ATP By similarity HAMAP-Rule MF_00420

Sequences

	Sequence		Length	Mass (Da)
	D5VRG1 [UniParc]. Last modified July 13, 2010. Version 1. Checksum: B74B583092B96D99	FASTA	722	80,097
	10 20 30 40 50 60 MDENDLKYIQ KTLGREINEI EKAMFENLWS EHCSYRTSKK LLRMFSKTIN ERTSKDIVVG 70 80 90 100 110 120 IGDDAAVIRL KDNICLALAM ESHNHPSYID PYNGAATGVG GIVRDVLSMG AKPIALLDPL 130 140 150 160 170 180 RFGDPRVEKV RWLIDGVVRG IGDYGNRIGV PTVGGECEFD ESFNYNNLVN VVCVGLVKEE 190 200 210 220 230 240 DIVRGKTKEK ELSLILVGST GRDGIGGASF ASKDLTEESE EERPSVQVGD AFSEKCLIDA 250 260 270 280 290 300 ILEAVKTKKV KAMKDLGAAG LSGAATEMCL SGCELYLENV PLREPLTPYE IMVSESQERM 310 320 330 340 350 360 LLAVEKGSEE EIIKIFEKYD LPAAVIGRTT DTGRIIAKYK GEVVVDLPIS LLCNAPLYDR 370 380 390 400 410 420 EEEERILEKE GRIEERVDLE EAILKLLESP NICSKEWIYS QYDYDVQLRT VIKPGRDAAV 430 440 450 460 470 480 LRILETYPVG IALTVDCNSR FCKLNPYVGS LYTVAEAVRN LACVGAKPLA MLDNLNFANP 490 500 510 520 530 540 EKPDRFWQLK ECIRGLADAA FSFDIPVVGG NVSLYNETVI EGKEYPINPT PAIALIGKID 550 560 570 580 590 600 NIEKVPGVLD NKVEEGDILI ITNETKEEMG GSEYYKVLFN SEIGRVPRVN LEKEKEIYKK 610 620 630 640 650 660 VVSLINRGLI SGTKDCSRGG LGIALAKFCI NNNIGVEVEL DNYNINKLRE DLLLFSETSG 670 680 690 700 710 720 RIILAVKEEN LNEVLKEIDG YVIGRVGGKE LSIKINNNKI SISLGEMKKV YKEAFPKMMG EL

References

[1]

"Complete sequence of Methanocaldococcus infernus ME." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Munk A.C., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Woyke T. Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11812 / JCM 15783 / ME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002009 Genomic DNA. Translation: ADG13164.1.
RefSeq	YP_003616128.1. NC_014122.1.
3D structure databases
ProteinModelPortal	D5VRG1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADG13164; ADG13164; Metin_0494.
GeneID	9131499.
KEGG	mif:Metin_0494.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
Enzyme and pathway databases
BioCyc	MINF573063:GHL3-501-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D5VRG1_METIM

Accession

Primary (citable) accession number: D5VRG1

Entry history

Integrated into UniProtKB/TrEMBL:	July 13, 2010
Last sequence update:	July 13, 2010
Last modified:	May 1, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)