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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Caulobacter segnis (strain ATCC 21756 / DSM 7131 / JCM 7823 / NBRC 15250 / LMG 17158 / TK0059) (Mycoplana segnis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotationSAAS annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Zinc 1UniRule annotation1
Metal bindingi58Zinc 1UniRule annotation1
Metal bindingi60Zinc 2UniRule annotation1
Metal bindingi61Zinc 2UniRule annotation1
Metal bindingi113Zinc 1UniRule annotation1
Metal bindingi132Zinc 1UniRule annotation1
Metal bindingi132Zinc 2UniRule annotation1
Metal bindingi170Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
LigandMetal-bindingUniRule annotationSAAS annotation, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCSEG509190:G1GKR-412-MONOMER.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
Ordered Locus Names:Cseg_0398Imported
OrganismiCaulobacter segnis (strain ATCC 21756 / DSM 7131 / JCM 7823 / NBRC 15250 / LMG 17158 / TK0059) (Mycoplana segnis)Imported
Taxonomic identifieri509190 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000002629 Componenti: Chromosome

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi509190.Cseg_0398.

Structurei

3D structure databases

ProteinModelPortaliD5VE93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 170Lactamase_BInterPro annotationAdd BLAST158

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
KOiK01069.
OMAiNYIWLLQ.
OrthoDBiPOG091H03YM.

Family and domain databases

CDDicd07723. hydroxyacylglutathione_hydrola. 1 hit.
Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiView protein in InterPro
IPR035680. Clx_II_MBL.
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
IPR036866. RibonucZ/Hydroxyglut_hydro.
PfamiView protein in Pfam
PF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

D5VE93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVTIHQFPC LSDNYGFLVR DEASGQVATI DTPDSETILT ALDALGWKLD
60 70 80 90 100
LILNTHWHPD HAGGNEALKA ATGAMIAGPS EVTRIAPVDR ALTDGDTVML
110 120 130 140 150
GETRFEVLDT GGHTLGHVSY HAPADGIAFV GDTLFALGCG RLFEGTPEQM
160 170 180 190 200
WASLSRLTAL PDQTTVYCAH EYTASNARFA LSVDDAPALK ARADAVFAAR
210 220 230 240
ERGEPTVPTT IAAEKATNPF LRAPLLRPNA ASPAEAFAEI RATKDSFKG
Length:249
Mass (Da):26,473
Last modified:July 13, 2010 - v1
Checksum:iFFE6383F99E5214E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002008 Genomic DNA. Translation: ADG08916.1.
RefSeqiWP_013077589.1. NC_014100.1.

Genome annotation databases

EnsemblBacteriaiADG08916; ADG08916; Cseg_0398.
KEGGicse:Cseg_0398.

Similar proteinsi

Entry informationi

Entry nameiD5VE93_CAUST
AccessioniPrimary (citable) accession number: D5VE93
Entry historyiIntegrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: March 28, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported