ID   D5V7L2_ARCNC            Unreviewed;       292 AA.
AC   D5V7L2;
DT   13-JUL-2010, integrated into UniProtKB/TrEMBL.
DT   13-JUL-2010, sequence version 1.
DT   24-JAN-2024, entry version 53.
DE   RecName: Full=Type-2 restriction enzyme {ECO:0000256|PIRNR:PIRNR016080};
DE            EC=3.1.21.4 {ECO:0000256|PIRNR:PIRNR016080};
GN   OrderedLocusNames=Arnit_2984 {ECO:0000313|EMBL:ADG94632.1};
OS   Arcobacter nitrofigilis (strain ATCC 33309 / DSM 7299 / CCUG 15893 / LMG
OS   7604 / NCTC 12251 / CI) (Campylobacter nitrofigilis).
OC   Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC   Arcobacteraceae; Arcobacter.
OX   NCBI_TaxID=572480 {ECO:0000313|EMBL:ADG94632.1, ECO:0000313|Proteomes:UP000000939};
RN   [1] {ECO:0000313|EMBL:ADG94632.1, ECO:0000313|Proteomes:UP000000939}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33309 / DSM 7299 / CCUG 15893 / LMG 7604 / NCTC 12251 / CI
RC   {ECO:0000313|Proteomes:UP000000939};
RX   PubMed=21304714; DOI=10.4056/sigs.912121;
RA   Pati A., Gronow S., Lapidus A., Copeland A., Glavina Del Rio T., Nolan M.,
RA   Lucas S., Tice H., Cheng J.F., Han C., Chertkov O., Bruce D., Tapia R.,
RA   Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D., Detter J.C.,
RA   Rohde M., Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Klenk H.P., Kyrpides N.C.;
RT   "Complete genome sequence of Arcobacter nitrofigilis type strain (CI).";
RL   Stand. Genomic Sci. 2:300-308(2010).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded unmethylated sequence 5'-GATC-3'.
CC       {ECO:0000256|PIRNR:PIRNR016080}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC         Evidence={ECO:0000256|PIRNR:PIRNR016080};
CC   -!- SIMILARITY: Belongs to the DpnII type II restriction endonuclease
CC       family. {ECO:0000256|PIRNR:PIRNR016080}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP001999; ADG94632.1; -; Genomic_DNA.
DR   RefSeq; WP_013136777.1; NC_014166.1.
DR   AlphaFoldDB; D5V7L2; -.
DR   STRING; 572480.Arnit_2984; -.
DR   REBASE; 26107; Ani7299ORF2986P.
DR   KEGG; ant:Arnit_2984; -.
DR   eggNOG; ENOG502Z7V5; Bacteria.
DR   HOGENOM; CLU_089327_0_0_7; -.
DR   OrthoDB; 9771872at2; -.
DR   Proteomes; UP000000939; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-UniRule.
DR   InterPro; IPR021191; Restrct_endonuc_II_DpnII.
DR   InterPro; IPR007637; Restrct_endonuc_II_DpnII-like.
DR   Pfam; PF04556; DpnII; 1.
DR   PIRSF; PIRSF016080; Restrict_endonuc_II_DpmII; 1.
PE   3: Inferred from homology;
KW   Endonuclease {ECO:0000256|PIRNR:PIRNR016080};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR016080, ECO:0000313|EMBL:ADG94632.1};
KW   Nuclease {ECO:0000256|PIRNR:PIRNR016080};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000939};
KW   Restriction system {ECO:0000256|PIRNR:PIRNR016080}.
FT   DOMAIN          2..288
FT                   /note="Restriction endonuclease type II DpnII-like"
FT                   /evidence="ECO:0000259|Pfam:PF04556"
SQ   SEQUENCE   292 AA;  34623 MW;  A92095119FA97371 CRC64;
     MDFRNFKNTL KDSIFTWDYF TNFNKVRLNV KKVEKELNIL NYLIGKENLE REFISLIEEY
     PNVRKALPLL IAVRTNKLIV TPVLTNIETL IPENKHYIFY DELNDEIKRE LIIFFNQTGL
     KEVFENKCIK NLVDYSFGVE VGFDTNARKN RTGTLMENIV AKFLDEFCEE NPEFTYIEQA
     TQKRILNTFN YNIDIDKNSR RFDFALFNRK KKKLYLIEVN YYSGGGSKLK ATAGEYQYLH
     NFLKMQNLDF IWITDGKGWL TALNPLEETF NHNDYVVNLE MLKNGILDEI IK
//