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Protein

Fructose-1,6-bisphosphate aldolase/phosphatase

Gene

fbp

Organism
Thermosphaera aggregans (strain DSM 11486 / M11TL)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).UniRule annotation

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.UniRule annotation
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13Proton acceptor; for FBP phosphatase activityUniRule annotation1
Metal bindingi13Magnesium 1UniRule annotation1
Metal bindingi20Magnesium 1; via pros nitrogenUniRule annotation1
Binding sitei20FBP; via tele nitrogenUniRule annotation1
Metal bindingi54Magnesium 1UniRule annotation1
Metal bindingi54Magnesium 2UniRule annotation1
Metal bindingi55Magnesium 2UniRule annotation1
Binding sitei92FBPUniRule annotation1
Metal bindingi96Magnesium 1UniRule annotation1
Metal bindingi133Magnesium 2UniRule annotation1
Binding sitei134FBP/DHAPUniRule annotation1
Active sitei230Proton donor/acceptor; for FBP aldolase activityUniRule annotation1
Active sitei233Schiff-base intermediate with DHAP; for FBP aldolase activityUniRule annotation1
Metal bindingi233Magnesium 3; via carbonyl oxygenUniRule annotation1
Metal bindingi234Magnesium 3UniRule annotation1
Metal bindingi234Magnesium 4UniRule annotation1
Metal bindingi235Magnesium 2UniRule annotation1
Metal bindingi235Magnesium 3UniRule annotation1
Binding sitei267FBP/DHAP; via amide nitrogenUniRule annotation1
Binding sitei288FBP/DHAPUniRule annotation1
Binding sitei349FBPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationImported, LyaseUniRule annotation
Biological processCarbohydrate metabolismUniRule annotation, GluconeogenesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation, Schiff baseUniRule annotation

Enzyme and pathway databases

BioCyciTAGG633148:GHBI-1265-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphate aldolase/phosphataseUniRule annotation (EC:3.1.3.11UniRule annotation, EC:4.1.2.13UniRule annotation)
Short name:
FBP A/PUniRule annotation
Short name:
FBP aldolase/phosphataseUniRule annotation
Gene namesi
Name:fbpUniRule annotation
Ordered Locus Names:Tagg_1241Imported
OrganismiThermosphaera aggregans (strain DSM 11486 / M11TL)Imported
Taxonomic identifieri633148 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeThermosphaera
Proteomesi
  • UP000002376 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; dimer of tetramers.UniRule annotation

Protein-protein interaction databases

STRINGi633148.Tagg_1241.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni105 – 106FBP bindingUniRule annotation2
Regioni243 – 244FBP binding; shared with dimeric partnerUniRule annotation2

Domaini

Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.UniRule annotation

Sequence similaritiesi

Belongs to the FBP aldolase/phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
HOGENOMiHOG000229394.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.

Sequencei

Sequence statusi: Complete.

D5U307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDKITLSII KADVGSIAGH HMPHPDQLAA ATKILAEAKQ KDIIIDFYVT
60 70 80 90 100
HVGDDIQLIM THRKGVDHPD IHGLAWEAFK SATKVAKELK LYAAGQDLLS
110 120 130 140 150
EAFSGNVRGM GPGVAELEME EREAEPVITF HADKTEPGAF NMPIYRIFAD
160 170 180 190 200
PFNTAGLVID PKMHDGFIFE VYDVFEGRSV LMKTPEESYD VLALIGTPGR
210 220 230 240 250
YIVRRVYRKS DNLLAAVVSV ERLNLTAGRY VGKDDPVAIV RAQHGLPAVG
260 270 280 290 300
EVLEPFSFPH LVAGWMRGSH HGPLMPVPMR YAKVTRFDGP PRIIALGWNI
310 320 330 340 350
SKGRLIGPVD LFDDVAFDET RRLAEMIAEY MRRHGPFMPH RLGPEEMEYT
360 370 380
TLPSVLEKLK DRFVKTEEVK VVKKHSDLLS H
Length:381
Mass (Da):42,534
Last modified:July 13, 2010 - v1
Checksum:iBAA86C23921BD26C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001939 Genomic DNA. Translation: ADG91507.1.
RefSeqiWP_013130100.1. NC_014160.1.

Genome annotation databases

EnsemblBacteriaiADG91507; ADG91507; Tagg_1241.
GeneIDi9166284.
KEGGitag:Tagg_1241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001939 Genomic DNA. Translation: ADG91507.1.
RefSeqiWP_013130100.1. NC_014160.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi633148.Tagg_1241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADG91507; ADG91507; Tagg_1241.
GeneIDi9166284.
KEGGitag:Tagg_1241.

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
HOGENOMiHOG000229394.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciTAGG633148:GHBI-1265-MONOMER.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiD5U307_THEAM
AccessioniPrimary (citable) accession number: D5U307
Entry historyiIntegrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: June 7, 2017
This is version 34 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.