Glyceraldehyde-3-phosphate dehydrogenase - Thermosphaera aggregans (strain DSM 11486 / M11TL)

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D5U0C7 (D5U0C7_THEAM) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 23. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase HAMAP-Rule MF_00559
Short name=GAPDH HAMAP-Rule MF_00559
EC=1.2.1.59 HAMAP-Rule MF_00559

Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase HAMAP-Rule MF_00559

Gene names

Name:	gap HAMAP-Rule MF_00559
Ordered Locus Names:	Tagg_0301

Organism

Thermosphaera aggregans (strain DSM 11486 / M11TL) [Complete proteome] [HAMAP] EMBL ADG90577.1

Taxonomic identifier

633148 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Thermosphaera ›

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)⁺ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP-Rule MF_00559 SAAS SAAS006436 RuleBase RU003388
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP-Rule MF_00559 SAAS SAAS006436 RuleBase RU003388
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00559 RuleBase RU003388 SAAS SAAS006436
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00559 RuleBase RU003388.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. HAMAP-Rule MF_00559 RuleBase RU000397

Ontologies

Keywords
Biological process	Glycolysis HAMAP-Rule MF_00559 SAAS SAAS006436 RuleBase RU003388
Cellular component	Cytoplasm HAMAP-Rule MF_00559 SAAS SAAS006436
Ligand	NAD HAMAP-Rule MF_00559 SAAS SAAS006436 RuleBase RU003388 NADP SAAS SAAS006436 HAMAP-Rule MF_00559 RuleBase RU003388
Molecular function	Oxidoreductase HAMAP-Rule MF_00559 SAAS SAAS006436 EMBL ADG90577.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

11 – 12

NAD By similarity HAMAP-Rule MF_00559

Region

138 – 140

Glyceraldehyde 3-phosphate binding By similarity HAMAP-Rule MF_00559

Region

193 – 194

Glyceraldehyde 3-phosphate binding By similarity HAMAP-Rule MF_00559

Sites

Active site

139

Nucleophile By similarity HAMAP-Rule MF_00559

Binding site

109

NAD; via amide nitrogen By similarity HAMAP-Rule MF_00559

Binding site

167

NAD By similarity HAMAP-Rule MF_00559

Binding site

301

NAD; via carbonyl oxygen By similarity HAMAP-Rule MF_00559

Sequences

Sequence

Length

Mass (Da)

Tools

D5U0C7 [UniParc].

Last modified July 13, 2010. Version 1.
Checksum: 356D92B0171D6F33
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FASTA

343

37,975

        10         20         30         40         50         60 
MVKVGVNGYG TIGKRVADAI LKNPRFQLVG IVKYNPDYSS KIAFKKGINI YVPQDKWRDF 

        70         80         90        100        110        120 
ESTGITPKGT IQEFLEESSI IIDASPNGKG AANLSLYKGA GKPAIFQGGE KPEVAELSYS 

       130        140        150        160        170        180 
TYCNYDEAFG RKYLRVVSCN TTGILRVLCA LHREFNVKRA RVLVIRRGAD PKEDARGPIN 

       190        200        210        220        230        240 
SIKLESFEDV SHHGKDAMLV LPSVKIISQA VVVPTTLMHV QYLDIDVASP VTRSQVIEAL 

       250        260        270        280        290        300 
SKHARILLVN PKKTGIDSTS KLIEAARDFL RSRNDIYENI VFENTIIVSD NNISLIQAIH 

       310        320        330        340 
QESIVVPENM DALYAIANIR LPFEKVVEET DRLLGVGGLK DVF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermosphaera aggregans type strain (M11TLT)." Spring S., Rachel R., Lapidus A., Davenport K., Tice H., Copeland A., Cheng J.-F., Lucas S., Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A. Klenk H.-P. Stand. Genomic Sci. 2:245-259(2010) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11486 / M11TL.
[2]	"Complete genome sequence of Thermosphaera aggregans type strain (M11TL)." US DOE Joint Genome Institute (JGI-PGF) Spring S., Lapidus A., Munk C., Schroeder M., Glavina Del Rio T., Tice H., Copeland A., Cheng J.-F., Lucas S., Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N., Mavromatis K., Ovchinnikova G., Pati A. Klenk H.-P. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 11486.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001939 Genomic DNA. Translation: ADG90577.1.
RefSeq	YP_003649529.1. NC_014160.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADG90577; ADG90577; Tagg_0301.
GeneID	9165314.
KEGG	tag:Tagg_0301.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000223361.
KO	K00150.
OMA	VPSHHGP.
Enzyme and pathway databases
UniPathway	UPA00109; UER00184.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_00559. G3P_dehdrog_arch.
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D5U0C7_THEAM

Accession

Primary (citable) accession number: D5U0C7

Entry history

Integrated into UniProtKB/TrEMBL:	July 13, 2010
Last sequence update:	July 13, 2010
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information