Phosphoenolpyruvate carboxykinase [GTP] - Thermosphaera aggregans (strain DSM 11486 / M11TL)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
Short name=PEP carboxykinase HAMAP-Rule MF_00452
Short name=PEPCK HAMAP-Rule MF_00452
EC=4.1.1.32 HAMAP-Rule MF_00452

Alternative name(s):
Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00452

Gene names

Name:	pckG HAMAP-Rule MF_00452
Ordered Locus Names:	Tagg_0003

Organism

Thermosphaera aggregans (strain DSM 11486 / M11TL) [Complete proteome] [HAMAP] EMBL ADG90286.1

Taxonomic identifier

633148 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Desulfurococcaceae › Thermosphaera ›

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452
Catalytic activity	GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO₂. HAMAP-Rule MF_00452
Cofactor	Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00452.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. HAMAP-Rule MF_00452

Ontologies

Keywords
Biological process	Gluconeogenesis HAMAP-Rule MF_00452
Cellular component	Cytoplasm HAMAP-Rule MF_00452
Ligand	GTP-binding HAMAP-Rule MF_00452 Manganese HAMAP-Rule MF_00452 Metal-binding HAMAP-Rule MF_00452 Nucleotide-binding Pyruvate EMBL ADG90286.1
Molecular function	Decarboxylase HAMAP-Rule MF_00452 Kinase EMBL ADG90286.1 Lyase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP kinase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxykinase (GTP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

391 – 393

3

Substrate binding By similarity HAMAP-Rule MF_00452

Sites

Active site

279

1

By similarity HAMAP-Rule MF_00452

Metal binding

236

1

Manganese By similarity HAMAP-Rule MF_00452

Metal binding

255

1

Manganese By similarity HAMAP-Rule MF_00452

Metal binding

295

1

Manganese By similarity HAMAP-Rule MF_00452

Binding site

94

1

Substrate By similarity HAMAP-Rule MF_00452

Binding site

229

1

Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00452

Binding site

236

1

Substrate By similarity HAMAP-Rule MF_00452

Binding site

393

1

GTP By similarity HAMAP-Rule MF_00452

Binding site

424

1

GTP By similarity HAMAP-Rule MF_00452

Sequences

Sequence

Length

Mass (Da)

Tools

D5TZI6 [UniParc].

Last modified July 13, 2010. Version 1.
Checksum: 04EF80C467CDB31E
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FASTA

630

72,093

        10         20         30         40         50         60 
METQGFCTES LNALKPYMSS RELGKLARIR DCSLVEWIRQ VVEHLKPSSI FINTGSEEDL 

        70         80         90        100        110        120 
DYIRRRAVEN KEEYVSSYNQ LHTVHFDGVR DLARDRGNTK IMTGKGVKIP LVNSGEREEL 

       130        140        150        160        170        180 
KKEVWEIYRD IMRGKEMFIG FYCFGPRGSP FTLYGVQVTD SAYVVHSENI LYRVCYDEFT 

       190        200        210        220        230        240 
RNDRIEYMRF LHSSGERDEN GWSKNISKRR IYIDPEEMIT YSVNTQYAGN TVGLKKLSLR 

       250        260        270        280        290        300 
LCVYKGFREG WLCEHMFIIG MKGSGDRVAY FTGAFPAGCG KTSTALIADL VVGDDLAIIR 

       310        320        330        340        350        360 
NVDGVARAVN PEVGMFGIID GVNPVDDAEI YKILTSRTSE VIFANVLYTE DGDVWWNGKE 

       370        380        390        400        410        420 
GAPKPGVNYA GEWWPGKRDE NGNPIPPSHP NARFTTSIFY LSRVDPRIND PMGVPVSGMI 

       430        440        450        460        470        480 
FGGRDSDTWV PVEEAFDWFH GIVTKGASLE SERTTAVLGQ AGVREFNPFA ILDFLSISPG 

       490        500        510        520        530        540 
AFIDLHFRFA RELSQSPKVY GVNYFLRGED GRFLTEKVDK RVWLRWMEKR VHGELDAVET 

       550        560        570        580        590        600 
PTGYIPIYSD LVDLFNKELG KEFPESLYEK LFTVRVKQHL DKIERIWRIY AEIPDTPALF 

       610        620        630 
FSILKEEKQR LVNARNRFGD FISPFRLDRA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermosphaera aggregans type strain (M11TLT)." Spring S., Rachel R., Lapidus A., Davenport K., Tice H., Copeland A., Cheng J.-F., Lucas S., Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A. Klenk H.-P. Stand. Genomic Sci. 2:245-259(2010) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11486 / M11TL.
[2]	"Complete genome sequence of Thermosphaera aggregans type strain (M11TL)." US DOE Joint Genome Institute (JGI-PGF) Spring S., Lapidus A., Munk C., Schroeder M., Glavina Del Rio T., Tice H., Copeland A., Cheng J.-F., Lucas S., Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N., Mavromatis K., Ovchinnikova G., Pati A. Klenk H.-P. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DSM 11486.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001939 Genomic DNA. Translation: ADG90286.1.
RefSeq	YP_003649238.1. NC_014160.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADG90286; ADG90286; Tagg_0003.
GeneID	9165007.
KEGG	tag:Tagg_0003.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000191700.
KO	K01596.
OMA	WMRFGED.
Enzyme and pathway databases
UniPathway	UPA00138.
Family and domain databases
Gene3D	3.40.449.10. 1 hit. 3.90.228.20. 2 hits.
HAMAP	MF_00452. PEPCK_GTP.
InterPro	IPR013035. PEP_carboxykinase_C. IPR008209. PEP_carboxykinase_GTP. IPR008210. PEP_carboxykinase_N. [Graphical view]
PANTHER	PTHR11561. PTHR11561. 1 hit.
Pfam	PF00821. PEPCK. 1 hit. [Graphical view]
PIRSF	PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D5TZI6_THEAM

Accession

Primary (citable) accession number: D5TZI6

Entry history

Integrated into UniProtKB/TrEMBL:	July 13, 2010
Last sequence update:	July 13, 2010
Last modified:	May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information