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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermosphaera aggregans (strain DSM 11486 / M11TL)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei94SubstrateUniRule annotation1
Metal bindingi236ManganeseUniRule annotation1
Metal bindingi255Manganese; via tele nitrogenUniRule annotation1
Active sitei279UniRule annotation1
Metal bindingi295ManganeseUniRule annotation1
Binding sitei393GTPUniRule annotation1
Binding sitei424GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi278 – 283GTPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotation, KinaseImported, Lyase, Transferase
Biological processGluconeogenesisUniRule annotation
LigandGTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinaseUniRule annotation
Short name:
GTP-PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Tagg_0003Imported
OrganismiThermosphaera aggregans (strain DSM 11486 / M11TL)Imported
Taxonomic identifieri633148 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeThermosphaera
Proteomesi
  • UP000002376 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi633148.Tagg_0003.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 246PEPCK_NInterPro annotationAdd BLAST211
Domaini251 – 611PEPCK_CInterPro annotationAdd BLAST361

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni227 – 229Substrate bindingUniRule annotation3
Regioni391 – 393Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiGPTNNWV.
OrthoDBiPOG093Z01SN.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiView protein in InterPro
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR035077. PEP_carboxykinase_GTP_C.
IPR035078. PEP_carboxykinase_GTP_N.
IPR008210. PEP_carboxykinase_N.
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiView protein in Pfam
PF00821. PEPCK_C. 1 hit.
PF17297. PEPCK_N. 1 hit.
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.

Sequencei

Sequence statusi: Complete.

D5TZI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METQGFCTES LNALKPYMSS RELGKLARIR DCSLVEWIRQ VVEHLKPSSI
60 70 80 90 100
FINTGSEEDL DYIRRRAVEN KEEYVSSYNQ LHTVHFDGVR DLARDRGNTK
110 120 130 140 150
IMTGKGVKIP LVNSGEREEL KKEVWEIYRD IMRGKEMFIG FYCFGPRGSP
160 170 180 190 200
FTLYGVQVTD SAYVVHSENI LYRVCYDEFT RNDRIEYMRF LHSSGERDEN
210 220 230 240 250
GWSKNISKRR IYIDPEEMIT YSVNTQYAGN TVGLKKLSLR LCVYKGFREG
260 270 280 290 300
WLCEHMFIIG MKGSGDRVAY FTGAFPAGCG KTSTALIADL VVGDDLAIIR
310 320 330 340 350
NVDGVARAVN PEVGMFGIID GVNPVDDAEI YKILTSRTSE VIFANVLYTE
360 370 380 390 400
DGDVWWNGKE GAPKPGVNYA GEWWPGKRDE NGNPIPPSHP NARFTTSIFY
410 420 430 440 450
LSRVDPRIND PMGVPVSGMI FGGRDSDTWV PVEEAFDWFH GIVTKGASLE
460 470 480 490 500
SERTTAVLGQ AGVREFNPFA ILDFLSISPG AFIDLHFRFA RELSQSPKVY
510 520 530 540 550
GVNYFLRGED GRFLTEKVDK RVWLRWMEKR VHGELDAVET PTGYIPIYSD
560 570 580 590 600
LVDLFNKELG KEFPESLYEK LFTVRVKQHL DKIERIWRIY AEIPDTPALF
610 620 630
FSILKEEKQR LVNARNRFGD FISPFRLDRA
Length:630
Mass (Da):72,093
Last modified:July 13, 2010 - v1
Checksum:i04EF80C467CDB31E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001939 Genomic DNA. Translation: ADG90286.1.
RefSeqiWP_013128879.1. NC_014160.1.

Genome annotation databases

EnsemblBacteriaiADG90286; ADG90286; Tagg_0003.
GeneIDi9165007.
KEGGitag:Tagg_0003.

Similar proteinsi

Entry informationi

Entry nameiD5TZI6_THEAM
AccessioniPrimary (citable) accession number: D5TZI6
Entry historyiIntegrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: September 27, 2017
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported