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D5TAU4 (D5TAU4_LEGP2) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_00098
EC=6.1.1.10 HAMAP-Rule MF_00098
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_00098
Gene names
Name:metG HAMAP-Rule MF_00098 EMBL ADG26279.1
Ordered Locus Names:lpa_04190
OrganismLegionella pneumophila serogroup 1 (strain 2300/99 Alcoy) [Complete proteome] [HAMAP]
Taxonomic identifier423212 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella ›

Protein attributes

Sequence length664 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098 SAAS SAAS002547

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098 SAAS SAAS002547

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00098 SAAS SAAS002547

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00098 SAAS SAAS002547

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00098 SAAS SAAS002547.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. HAMAP-Rule MF_00098

Contains 1 tRNA-binding domain. HAMAP-Rule MF_00098 SAAS SAAS002547

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain564 – 664101tRNA-binding By similarity HAMAP-Rule MF_00098
Motif8 – 1811"HIGH" region By similarity HAMAP-Rule MF_00098
Motif324 – 3285"KMSKS" region By similarity HAMAP-Rule MF_00098

Sites

Metal binding1391Zinc By similarity HAMAP-Rule MF_00098
Metal binding1421Zinc By similarity HAMAP-Rule MF_00098
Metal binding1521Zinc By similarity HAMAP-Rule MF_00098
Metal binding1551Zinc By similarity HAMAP-Rule MF_00098
Binding site3271ATP By similarity HAMAP-Rule MF_00098

Sequences

Sequence LengthMass (Da)Tools
D5TAU4 [UniParc].

Last modified July 13, 2010. Version 1.
Checksum: 0533D15652A783A2

FASTA66475,194
        10         20         30         40         50         60 
MLVTSALPYA NGHLHLGHLV EHIQTDIWVR THKMLGIQCI SVCGDDAHGT PIMLKAEQLG 

        70         80         90        100        110        120 
ITPEALTAEI KLSHEKDFKA FAIDYDYYHT THSPENQALA TTIFERLQAG GDIVKKTIRQ 

       130        140        150        160        170        180 
FYDPVKQMFL PDRYVKGTCP KCAAVDQYGD NCEVCGATYS PTDLINPVSV ISGASPVEME 

       190        200        210        220        230        240 
SEHYFFDLPR YEELLKDWTR KGHLQTEVTN KLSEWFEAGL KQWDISRDAP YFGFPIPGVP 

       250        260        270        280        290        300 
DKYFYVWLDA PIGYMASFKK YCDERGVSFD EFWDKASKTE LYHFVGKDIV YFHALFWPAM 

       310        320        330        340        350        360 
LAASGFRTPT AVYTHGFLTV EGQKMSKSRG TFIEARAYLA HLHPEYLRYY FAAKLNGRVD 

       370        380        390        400        410        420 
DLDLNFDDFV NRVNADLVGK VVNIASRCAG FINKRFDNRL SSELINQKLY NDLLSAREFV 

       430        440        450        460        470        480 
IDAFVSRDYA RAIRQIMDCA DKVNQYIDAN KPWVLAKDES KLNEVHAICT MGINLFRILI 

       490        500        510        520        530        540 
TYLKPVLPMM AKASEEFLNS EPLHWGSIDK PLLNHRINTF KPLMVRVEKE KIEAMLVQSK 

       550        560        570        580        590        600 
ESLMTTPIKE NTPVEDSNLI SIEDFAKVDL RIAKIVNAEP VEGADKLMRL ILDLGDAQKQ 

       610        620        630        640        650        660 
VFAGIKKAYD AEELIGRLTV MVANLEPRTM RFGVSEGMVL AAGDGQGIYL LQPDAGAFPG 


MKVK

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References

[1]"Legionella pneumophila pangenome reveals strain-specific virulence factors."
D'Auria G., Jimenez-Hernandez N., Peris-Bondia F., Moya A., Latorre A.
BMC Genomics 11:181-181(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2300/99 Alcoy EMBL ADG26279.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001828 Genomic DNA. Translation: ADG26279.1.
RefSeqYP_003620231.1. NC_014125.1.

3D structure databases

ProteinModelPortalD5TAU4.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADG26279; ADG26279; lpa_04190.
GeneID9130839.
KEGGlpa:lpa_04190.
PATRIC38184211. VBILegPne70474_3188.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000200400.
KOK01874.

Enzyme and pathway databases

BioCycLPNE423212:GHRR-3085-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00098. Met_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD5TAU4_LEGP2
AccessionPrimary (citable) accession number: D5TAU4
Entry history
Integrated into UniProtKB/TrEMBL: July 13, 2010
Last sequence update: July 13, 2010
Last modified: May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info