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Protein

Zinc metalloproteinase-disintegrin-like atrase-A

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Snake venom zinc metalloproteinase that inhibits platelet aggregation by cleaving platelet glycoprotein Ib alpha (GP1BA) at Glu-298/Asp-299, and abolishes binding of von Willebrand factor (VWF) to GPIBA.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi208Calcium 1By similarity1
Metal bindingi290Calcium 1By similarity1
Metal bindingi338Zinc; catalyticBy similarity1
Metal bindingi342Zinc; catalyticBy similarity1
Metal bindingi348Zinc; catalyticBy similarity1
Metal bindingi393Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi396Calcium 1By similarity1
Metal bindingi411Calcium 2By similarity1
Metal bindingi413Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi415Calcium 2By similarity1
Metal bindingi418Calcium 2By similarity1
Metal bindingi421Calcium 2By similarity1
Metal bindingi472Calcium 3By similarity1
Metal bindingi473Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi475Calcium 3By similarity1
Metal bindingi487Calcium 3By similarity1
Metal bindingi488Calcium 3; via carbonyl oxygenBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandCalcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.159.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase-disintegrin-like atrase-A (EC:3.4.24.-)
Alternative name(s):
Snake venom metalloproteinase
Short name:
SVMP
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000041802921 – 196By similarityAdd BLAST176
ChainiPRO_0000418030197 – 607Zinc metalloproteinase-disintegrin-like atrase-AAdd BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi314 ↔ 393By similarity
Disulfide bondi353 ↔ 377By similarity
Disulfide bondi355 ↔ 360By similarity
Disulfide bondi409 ↔ 438By similarity
Disulfide bondi420 ↔ 433By similarity
Disulfide bondi422 ↔ 428By similarity
Disulfide bondi432 ↔ 455By similarity
Glycosylationi434N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi446 ↔ 452By similarity
Disulfide bondi451 ↔ 477By similarity
Disulfide bondi464 ↔ 484By similarity
Disulfide bondi471 ↔ 503By similarity
Disulfide bondi496 ↔ 508By similarity
Disulfide bondi515 ↔ 565By similarity
Glycosylationi522N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi530 ↔ 573By similarity
Disulfide bondi543 ↔ 553By similarity
Disulfide bondi560 ↔ 599By similarity
Disulfide bondi593 ↔ 604By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliD5LMJ3.
SMRiD5LMJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini205 – 398Peptidase M12BPROSITE-ProRule annotationAdd BLAST194
Domaini406 – 492DisintegrinPROSITE-ProRule annotationAdd BLAST87

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi470 – 472D/ECD-tripeptide3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi493 – 607Cys-richAdd BLAST115

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR006586. ADAM_Cys-rich.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D5LMJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQALLVIIC LAVFPHQGSS IILESGNVND YEVVYPQKVP ALLKGGVQNP
60 70 80 90 100
QPETKYEDTM RYEFQVNGEP VVLHLERNKG LFSEDYTETH YAPDGREITT
110 120 130 140 150
SPPVQDHCYY HGYIQNEADS SAVISACNGL KGHFKHQGET YFIEPLELSE
160 170 180 190 200
SEAHAIYKDE NVEKEDETPK ICAVTQTTWE SDESIEKTSQ LTNTPEQDRY
210 220 230 240 250
LQVKKYIEFY LVVDNKMYKN HTSNQELRTR VYEMVNYLNT KYRRLNFHIA
260 270 280 290 300
LIGLEIWSNQ DKVDMDPGAN VTLKSFAEWR AKLPPHKRND NAQLLTGIDF
310 320 330 340 350
NGTTVGLAYT GTLCTWGSVA VVQDYSRRTI LMASTMAHEL GHNMGIHHDK
360 370 380 390 400
ANCRCSHSPC IMSDTISDEP FYEFSSCSVR EHQEYLLRER PQCILNKPSR
410 420 430 440 450
KAIVSRPVCG NNFVEVGEQC DCGSLQDCQS TCCNATTCKL QPHAQCDSEE
460 470 480 490 500
CCEKCKFKGA ETECRAAKDD CDLPEFCTGQ SAECPTDSLQ RNGHPCQNNQ
510 520 530 540 550
GYCYNGKCPT MENQCITLLG PNYTVGPAGC FKNNRKGDDV SHCRKENGAK
560 570 580 590 600
IPCAAKDEKC GTLYCTEIKK TGCIVPVSPR DPDSRMVEPG TKCEDKKVCS

KSQCVKV
Length:607
Mass (Da):68,254
Last modified:June 15, 2010 - v1
Checksum:i38E9A800C2FD7D65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU944972 mRNA. Translation: ADF43026.1.

Similar proteinsi

Entry informationi

Entry nameiVM3A_NAJAT
AccessioniPrimary (citable) accession number: D5LMJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 15, 2010
Last modified: October 25, 2017
This is version 34 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families