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D5JGG0

- BGAL_BACCO

UniProt

D5JGG0 - BGAL_BACCO

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Protein
Beta-galactosidase LacZ
Gene
lacZ
Organism
Bacillus coagulans
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101Substrate By similarity
Metal bindingi114 – 1141Zinc By similarity
Binding sitei148 – 1481Substrate By similarity
Active sitei149 – 1491Proton donor By similarity
Metal bindingi157 – 1571Zinc By similarity
Metal bindingi159 – 1591Zinc By similarity
Metal bindingi162 – 1621Zinc By similarity
Active sitei303 – 3031Nucleophile By similarity
Binding sitei311 – 3111Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase LacZ (EC:3.2.1.23)
Short name:
Beta-gal
Gene namesi
Name:lacZ
OrganismiBacillus coagulans
Taxonomic identifieri1398 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 665665Beta-galactosidase LacZ
PRO_0000407682Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliD5JGG0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni351 – 3544Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

D5JGG0-1 [UniParc]FASTAAdd to Basket

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MLKKHEKFYY GGDYNPEQWD ESVWKEDMRL MKKAGVNYVS INIFSWARLQ    50
PDEETYDFST LDKIMDMLAE NGIGADLATA TAAPPAWLSR KYPDSLPVDK 100
DGSRFLPGSR QHYCPNSKDY ARLAAKLVRK IAERYKSHPA LVMWHVNNEY 150
GCHISECYCD NCKKGFQTWL KEKYGTIENL NKSWSTDFWS QRYYEWEEIC 200
LPGKTPTFAN PMQQLDYKAF MDDSLLALYK MERDILKTYT PDVPVMTNLM 250
GLHKPVDGFH WAKEMDLVTW DAYPDPFEDI PYAQFMAHDL TRSLKKQPFL 300
LMEQAAGAVN WRAQNAVKAP GVMRLWSYEA AAHGADGIMF FQWRASQGGA 350
EKFHSGMVPH SGDEESRNFR EVVQLGNELK NLEKVTGSAY ASDVAIVFDW 400
KNWWALELDS KPSSLVTYIK QLLPFYRVLH TQNIGVDFIH PDEAMDRYKV 450
VFAPASYRVT KTFADKVKAY VENGGYFATN FFSGIADENE RVYLGGYPGA 500
YRDILGIYVE EFAPMKKGAV HQIRTGYGDA AIRVWEEKIH LKGAEALAWF 550
KDGYLAGSPA VTAHHCGKGK AYYIGTQPDE QYLSSLLKEI LKEADVRPAL 600
DAPRGVEVAV RKNGHEKFLF LLNHTDQVQF VDAGGTYPEL IYGRTEAETV 650
RLSPRDVKIL QVIEK 665
Length:665
Mass (Da):76,013
Last modified:June 15, 2010 - v1
Checksum:iE1DBF3B4E13FFAB4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GU323918 Genomic DNA. Translation: ADF47156.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
GU323918 Genomic DNA. Translation: ADF47156.1 .

3D structure databases

ProteinModelPortali D5JGG0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans."
    Kovacs A.T., van Hartskamp M., Kuipers O.P., van Kranenburg R.
    Appl. Environ. Microbiol. 76:4085-4088(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7050 / DSM 1 / JCM 2257 / NCIB 9365 / NCTC 10334.

Entry informationi

Entry nameiBGAL_BACCO
AccessioniPrimary (citable) accession number: D5JGG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 15, 2010
Last modified: June 11, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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