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D5JGG0

- BGAL_BACCO

UniProt

D5JGG0 - BGAL_BACCO

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Protein

Beta-galactosidase LacZ

Gene

lacZ

Organism
Bacillus coagulans
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101SubstrateBy similarity
Metal bindingi114 – 1141ZincBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Active sitei149 – 1491Proton donorBy similarity
Metal bindingi157 – 1571ZincBy similarity
Metal bindingi159 – 1591ZincBy similarity
Metal bindingi162 – 1621ZincBy similarity
Active sitei303 – 3031NucleophileBy similarity
Binding sitei311 – 3111SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase LacZ (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:lacZImported
OrganismiBacillus coagulans
Taxonomic identifieri1398 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 665665Beta-galactosidase LacZPRO_0000407682Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliD5JGG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni351 – 3544Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

D5JGG0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKKHEKFYY GGDYNPEQWD ESVWKEDMRL MKKAGVNYVS INIFSWARLQ
60 70 80 90 100
PDEETYDFST LDKIMDMLAE NGIGADLATA TAAPPAWLSR KYPDSLPVDK
110 120 130 140 150
DGSRFLPGSR QHYCPNSKDY ARLAAKLVRK IAERYKSHPA LVMWHVNNEY
160 170 180 190 200
GCHISECYCD NCKKGFQTWL KEKYGTIENL NKSWSTDFWS QRYYEWEEIC
210 220 230 240 250
LPGKTPTFAN PMQQLDYKAF MDDSLLALYK MERDILKTYT PDVPVMTNLM
260 270 280 290 300
GLHKPVDGFH WAKEMDLVTW DAYPDPFEDI PYAQFMAHDL TRSLKKQPFL
310 320 330 340 350
LMEQAAGAVN WRAQNAVKAP GVMRLWSYEA AAHGADGIMF FQWRASQGGA
360 370 380 390 400
EKFHSGMVPH SGDEESRNFR EVVQLGNELK NLEKVTGSAY ASDVAIVFDW
410 420 430 440 450
KNWWALELDS KPSSLVTYIK QLLPFYRVLH TQNIGVDFIH PDEAMDRYKV
460 470 480 490 500
VFAPASYRVT KTFADKVKAY VENGGYFATN FFSGIADENE RVYLGGYPGA
510 520 530 540 550
YRDILGIYVE EFAPMKKGAV HQIRTGYGDA AIRVWEEKIH LKGAEALAWF
560 570 580 590 600
KDGYLAGSPA VTAHHCGKGK AYYIGTQPDE QYLSSLLKEI LKEADVRPAL
610 620 630 640 650
DAPRGVEVAV RKNGHEKFLF LLNHTDQVQF VDAGGTYPEL IYGRTEAETV
660
RLSPRDVKIL QVIEK
Length:665
Mass (Da):76,013
Last modified:June 15, 2010 - v1
Checksum:iE1DBF3B4E13FFAB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU323918 Genomic DNA. Translation: ADF47156.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU323918 Genomic DNA. Translation: ADF47156.1 .

3D structure databases

ProteinModelPortali D5JGG0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans."
    Kovacs A.T., van Hartskamp M., Kuipers O.P., van Kranenburg R.
    Appl. Environ. Microbiol. 76:4085-4088(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 7050 / DSM 1 / JCM 2257 / NCIB 9365 / NCTC 10334Imported.

Entry informationi

Entry nameiBGAL_BACCO
AccessioniPrimary (citable) accession number: D5JGG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 15, 2010
Last modified: October 1, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3