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Protein

Beta-galactosidase LacZ

Gene

lacZ

Organism
Bacillus coagulans
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110SubstrateBy similarity1
Metal bindingi114ZincBy similarity1
Binding sitei148SubstrateBy similarity1
Active sitei149Proton donorBy similarity1
Metal bindingi157ZincBy similarity1
Metal bindingi159ZincBy similarity1
Metal bindingi162ZincBy similarity1
Active sitei303NucleophileBy similarity1
Binding sitei311SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding, Zinc

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase LacZ (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:lacZImported
OrganismiBacillus coagulans
Taxonomic identifieri1398 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004076821 – 665Beta-galactosidase LacZAdd BLAST665

Structurei

3D structure databases

ProteinModelPortaliD5JGG0.
SMRiD5JGG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni351 – 354Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence analysis

Phylogenomic databases

eggNOGiENOG4105C4C. Bacteria.
COG1874. LUCA.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiView protein in InterPro
IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

D5JGG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKKHEKFYY GGDYNPEQWD ESVWKEDMRL MKKAGVNYVS INIFSWARLQ
60 70 80 90 100
PDEETYDFST LDKIMDMLAE NGIGADLATA TAAPPAWLSR KYPDSLPVDK
110 120 130 140 150
DGSRFLPGSR QHYCPNSKDY ARLAAKLVRK IAERYKSHPA LVMWHVNNEY
160 170 180 190 200
GCHISECYCD NCKKGFQTWL KEKYGTIENL NKSWSTDFWS QRYYEWEEIC
210 220 230 240 250
LPGKTPTFAN PMQQLDYKAF MDDSLLALYK MERDILKTYT PDVPVMTNLM
260 270 280 290 300
GLHKPVDGFH WAKEMDLVTW DAYPDPFEDI PYAQFMAHDL TRSLKKQPFL
310 320 330 340 350
LMEQAAGAVN WRAQNAVKAP GVMRLWSYEA AAHGADGIMF FQWRASQGGA
360 370 380 390 400
EKFHSGMVPH SGDEESRNFR EVVQLGNELK NLEKVTGSAY ASDVAIVFDW
410 420 430 440 450
KNWWALELDS KPSSLVTYIK QLLPFYRVLH TQNIGVDFIH PDEAMDRYKV
460 470 480 490 500
VFAPASYRVT KTFADKVKAY VENGGYFATN FFSGIADENE RVYLGGYPGA
510 520 530 540 550
YRDILGIYVE EFAPMKKGAV HQIRTGYGDA AIRVWEEKIH LKGAEALAWF
560 570 580 590 600
KDGYLAGSPA VTAHHCGKGK AYYIGTQPDE QYLSSLLKEI LKEADVRPAL
610 620 630 640 650
DAPRGVEVAV RKNGHEKFLF LLNHTDQVQF VDAGGTYPEL IYGRTEAETV
660
RLSPRDVKIL QVIEK
Length:665
Mass (Da):76,013
Last modified:June 15, 2010 - v1
Checksum:iE1DBF3B4E13FFAB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU323918 Genomic DNA. Translation: ADF47156.1.

Genome annotation databases

GeneIDi29812235.

Similar proteinsi

Entry informationi

Entry nameiBGAL_BACCO
AccessioniPrimary (citable) accession number: D5JGG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 15, 2010
Last modified: November 22, 2017
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families