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D5JGG0 (BGAL_BACCO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase LacZ

Short name=Beta-gal
EC=3.2.1.23
Gene names
Name:lacZ
OrganismBacillus coagulans
Taxonomic identifier1398 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length665 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 665665Beta-galactosidase LacZ
PRO_0000407682

Regions

Region351 – 3544Substrate binding By similarity

Sites

Active site1491Proton donor By similarity
Active site3031Nucleophile By similarity
Metal binding1141Zinc By similarity
Metal binding1571Zinc By similarity
Metal binding1591Zinc By similarity
Metal binding1621Zinc By similarity
Binding site1101Substrate By similarity
Binding site1481Substrate By similarity
Binding site3111Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
D5JGG0 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: E1DBF3B4E13FFAB4

FASTA66576,013
        10         20         30         40         50         60 
MLKKHEKFYY GGDYNPEQWD ESVWKEDMRL MKKAGVNYVS INIFSWARLQ PDEETYDFST 

        70         80         90        100        110        120 
LDKIMDMLAE NGIGADLATA TAAPPAWLSR KYPDSLPVDK DGSRFLPGSR QHYCPNSKDY 

       130        140        150        160        170        180 
ARLAAKLVRK IAERYKSHPA LVMWHVNNEY GCHISECYCD NCKKGFQTWL KEKYGTIENL 

       190        200        210        220        230        240 
NKSWSTDFWS QRYYEWEEIC LPGKTPTFAN PMQQLDYKAF MDDSLLALYK MERDILKTYT 

       250        260        270        280        290        300 
PDVPVMTNLM GLHKPVDGFH WAKEMDLVTW DAYPDPFEDI PYAQFMAHDL TRSLKKQPFL 

       310        320        330        340        350        360 
LMEQAAGAVN WRAQNAVKAP GVMRLWSYEA AAHGADGIMF FQWRASQGGA EKFHSGMVPH 

       370        380        390        400        410        420 
SGDEESRNFR EVVQLGNELK NLEKVTGSAY ASDVAIVFDW KNWWALELDS KPSSLVTYIK 

       430        440        450        460        470        480 
QLLPFYRVLH TQNIGVDFIH PDEAMDRYKV VFAPASYRVT KTFADKVKAY VENGGYFATN 

       490        500        510        520        530        540 
FFSGIADENE RVYLGGYPGA YRDILGIYVE EFAPMKKGAV HQIRTGYGDA AIRVWEEKIH 

       550        560        570        580        590        600 
LKGAEALAWF KDGYLAGSPA VTAHHCGKGK AYYIGTQPDE QYLSSLLKEI LKEADVRPAL 

       610        620        630        640        650        660 
DAPRGVEVAV RKNGHEKFLF LLNHTDQVQF VDAGGTYPEL IYGRTEAETV RLSPRDVKIL 


QVIEK 

« Hide

References

[1]"Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans."
Kovacs A.T., van Hartskamp M., Kuipers O.P., van Kranenburg R.
Appl. Environ. Microbiol. 76:4085-4088(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 7050 / DSM 1 / JCM 2257 / NCIB 9365 / NCTC 10334.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
GU323918 Genomic DNA. Translation: ADF47156.1.

3D structure databases

ProteinModelPortalD5JGG0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL_BACCO
AccessionPrimary (citable) accession number: D5JGG0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 15, 2010
Last modified: June 11, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries