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D5JGG0

- BGAL_BACCO

UniProt

D5JGG0 - BGAL_BACCO

Protein

Beta-galactosidase LacZ

Gene

lacZ

Organism
Bacillus coagulans
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 14 (01 Oct 2014)
      Sequence version 1 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101SubstrateBy similarity
    Metal bindingi114 – 1141ZincBy similarity
    Binding sitei148 – 1481SubstrateBy similarity
    Active sitei149 – 1491Proton donorBy similarity
    Metal bindingi157 – 1571ZincBy similarity
    Metal bindingi159 – 1591ZincBy similarity
    Metal bindingi162 – 1621ZincBy similarity
    Active sitei303 – 3031NucleophileBy similarity
    Binding sitei311 – 3111SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase LacZ (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:lacZImported
    OrganismiBacillus coagulans
    Taxonomic identifieri1398 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 665665Beta-galactosidase LacZPRO_0000407682Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliD5JGG0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni351 – 3544Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D5JGG0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKKHEKFYY GGDYNPEQWD ESVWKEDMRL MKKAGVNYVS INIFSWARLQ    50
    PDEETYDFST LDKIMDMLAE NGIGADLATA TAAPPAWLSR KYPDSLPVDK 100
    DGSRFLPGSR QHYCPNSKDY ARLAAKLVRK IAERYKSHPA LVMWHVNNEY 150
    GCHISECYCD NCKKGFQTWL KEKYGTIENL NKSWSTDFWS QRYYEWEEIC 200
    LPGKTPTFAN PMQQLDYKAF MDDSLLALYK MERDILKTYT PDVPVMTNLM 250
    GLHKPVDGFH WAKEMDLVTW DAYPDPFEDI PYAQFMAHDL TRSLKKQPFL 300
    LMEQAAGAVN WRAQNAVKAP GVMRLWSYEA AAHGADGIMF FQWRASQGGA 350
    EKFHSGMVPH SGDEESRNFR EVVQLGNELK NLEKVTGSAY ASDVAIVFDW 400
    KNWWALELDS KPSSLVTYIK QLLPFYRVLH TQNIGVDFIH PDEAMDRYKV 450
    VFAPASYRVT KTFADKVKAY VENGGYFATN FFSGIADENE RVYLGGYPGA 500
    YRDILGIYVE EFAPMKKGAV HQIRTGYGDA AIRVWEEKIH LKGAEALAWF 550
    KDGYLAGSPA VTAHHCGKGK AYYIGTQPDE QYLSSLLKEI LKEADVRPAL 600
    DAPRGVEVAV RKNGHEKFLF LLNHTDQVQF VDAGGTYPEL IYGRTEAETV 650
    RLSPRDVKIL QVIEK 665
    Length:665
    Mass (Da):76,013
    Last modified:June 15, 2010 - v1
    Checksum:iE1DBF3B4E13FFAB4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GU323918 Genomic DNA. Translation: ADF47156.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    GU323918 Genomic DNA. Translation: ADF47156.1 .

    3D structure databases

    ProteinModelPortali D5JGG0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans."
      Kovacs A.T., van Hartskamp M., Kuipers O.P., van Kranenburg R.
      Appl. Environ. Microbiol. 76:4085-4088(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 7050 / DSM 1 / JCM 2257 / NCIB 9365 / NCTC 10334Imported.

    Entry informationi

    Entry nameiBGAL_BACCO
    AccessioniPrimary (citable) accession number: D5JGG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3