Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Germacrene A oxidase

Gene
N/A
Organism
Barnadesia spinosa (Spiny barnadesia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of germacrene-derived sesquiterpene lactones. Catalyzes three consecutive oxidations of germacrene A to produce germacrene A acid. Could also catalyze the three-step oxidation of non-natural substrate amorphadiene to artemisinic acid.

Catalytic activityi

+-germacrene A + NADPH + O2 = germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O.1 Publication
Germacra-1(10),4,11(13)-trien-12-ol + 2 NADP+ + H2O = germacra-1(10),4,11(13)-trien-12-oate + 2 NADPH.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi432Iron (heme axial ligand)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15756.
BRENDAi1.14.13.123. 12601.

Names & Taxonomyi

Protein namesi
Recommended name:
Germacrene A oxidase
Short name:
BsGAO
Alternative name(s):
Germacrene A alcohol dehydrogenase (EC:1.1.1.3141 Publication)
Germacrene A hydroxylase (EC:1.14.13.1231 Publication)
OrganismiBarnadesia spinosa (Spiny barnadesia)
Taxonomic identifieri171760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeBarnadesioideaeBarnadesia

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini24 – 496LumenalSequence analysisAdd BLAST473

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004127641 – 496Germacrene A oxidaseAdd BLAST496

Structurei

3D structure databases

ProteinModelPortaliD5JBX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5JBX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELTLTTSLG LAVFVFILFK LLTGSKSTKN SLPEAWRLPI IGHMHHLVGT
60 70 80 90 100
LPHRGVTDMA RKYGSLMHLQ LGEVSTIVVS SPRWAKEVLT TYDITFANRP
110 120 130 140 150
ETLTGEIVAY HNTDIVLSPY GEYWRQLRKL CTLELLSAKK VKSFQSLREE
160 170 180 190 200
ECWNLVKEVR SSGSGSPVDL SESIFKLIAT ILSRAAFGKG IKDQREFTEI
210 220 230 240 250
VKEILRLTGG FDVADIFPSK KILHHLSGKR AKLTNIHNKL DSLINNIVSE
260 270 280 290 300
HPGSRTSSSQ ESLLDVLLRL KDSAELPLTS DNVKAVILDM FGAGTDTSSA
310 320 330 340 350
TIEWAISELI RCPRAMEKVQ TELRQALNGK ERIQEEDIQE LSYLKLVIKE
360 370 380 390 400
TLRLHPPLPL VMPRECREPC VLAGYEIPTK TKLIVNVFAI NRDPEYWKDA
410 420 430 440 450
ETFMPERFEN SPINIMGSEY EYLPFGAGRR MCPGAALGLA NVELPLAHIL
460 470 480 490
YYFNWKLPNG ARLDELDMSE CFGATVQRKS ELLLVPTAYK TANNSA
Length:496
Mass (Da):55,699
Last modified:June 15, 2010 - v1
Checksum:i028C2D4B45ED6909
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU256647 mRNA. Translation: ADF43083.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU256647 mRNA. Translation: ADF43083.1.

3D structure databases

ProteinModelPortaliD5JBX1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15756.
BRENDAi1.14.13.123. 12601.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAO_BARSP
AccessioniPrimary (citable) accession number: D5JBX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: June 15, 2010
Last modified: July 6, 2016
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.