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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Salinibacter ruber (strain M8)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi114 – 1141Magnesium; catalyticUniRule annotation
Sitei115 – 1151Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei138 – 1381Proton acceptorUniRule annotation
Binding sitei173 – 1731Substrate; shared with dimeric partnerUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation
Binding sitei277 – 2771Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 792ATPUniRule annotation
Nucleotide bindingi113 – 1164ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSRUB761659:GHC6-2366-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotationSAAS annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:SRM_02332Imported
OrganismiSalinibacter ruber (strain M8)Imported
Taxonomic identifieri761659 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeSalinibacter
ProteomesiUP000000933 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Proteomic databases

PRIDEiD5HB48.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD5HB48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 1383Substrate bindingUniRule annotation
Regioni180 – 1823Substrate bindingUniRule annotation
Regioni283 – 2864Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248869.
KOiK00850.
PhylomeDBiD5HB48.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5HB48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSDSLRVGV LTGGGDCPGI NAALRAVTKS LTLQHDAEVL GFLDGFRGVV
60 70 80 90 100
DRNVEPLHYR DVSGILTRGG TILGASNKAD PFNYLPRGGA DVSAQVMRTY
110 120 130 140 150
RTLDLDALVA IGGDGTMSIA HRLTDLGVDI VGVPKTIDND LVGTDRTVGF
160 170 180 190 200
DTAVSTATDA IDRIHTTAQS HHRVMIVETM GRYSGWIALH AGVASGTDVI
210 220 230 240 250
LLPEIEYEVE TVADVCRDRE RSGQRFTIVA VAEGARRKEG DYVVQERGDE
260 270 280 290 300
YTDSIRLGGI SRVLADQLRE HLDTSIRTTV LGHMQRGGSP TAYDRTLATT
310 320 330 340 350
LGTRAATLVA EGTTGVMVAV QNDAFTQVPL DEIAGKTRTV PLDDPLIASG
360 370
LDVGTSFGVA VSDLSEAASH SKPASPIQP
Length:379
Mass (Da):40,144
Last modified:June 15, 2010 - v1
Checksum:i61A6205E6B33A745
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP565814 Genomic DNA. Translation: CBH25253.1.
RefSeqiWP_011404839.1. NC_014032.1.
YP_003572205.1. NC_014032.1.

Genome annotation databases

EnsemblBacteriaiCBH25253; CBH25253; SRM_02332.
KEGGisrm:SRM_02332.
PATRICi35492548. VBISalRub49863_2254.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP565814 Genomic DNA. Translation: CBH25253.1.
RefSeqiWP_011404839.1. NC_014032.1.
YP_003572205.1. NC_014032.1.

3D structure databases

ProteinModelPortaliD5HB48.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiD5HB48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBH25253; CBH25253; SRM_02332.
KEGGisrm:SRM_02332.
PATRICi35492548. VBISalRub49863_2254.

Phylogenomic databases

HOGENOMiHOG000248869.
KOiK00850.
PhylomeDBiD5HB48.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciSRUB761659:GHC6-2366-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fine-scale evolution: genomic, phenotypic and ecological differentiation in two coexisting Salinibacter ruber strains."
    Pena A., Teeling H., Huerta-Cepas J., Santos F., Yarza P., Brito-Echeverria J., Lucio M., Schmitt-Kopplin P., Meseguer I., Schenowitz C., Dossat C., Barbe V., Dopazo J., Rossello-Mora R., Schuler M., Glockner F.O., Amann R., Gabaldon T., Anton J.
    ISME J. -:0-0(2010)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: M8.
  2. "Genome sequence of Salinibacter ruber M8."
    Genoscope
    Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: M8Imported.

Entry informationi

Entry nameiD5HB48_SALRM
AccessioniPrimary (citable) accession number: D5HB48
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: June 24, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.