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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Salinibacter ruber (strain M8)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Transaldolase (tal)
  3. ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATP; via amide nitrogenUniRule annotation1
Metal bindingi114Magnesium; catalyticUniRule annotation1
Sitei115Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei138Proton acceptorUniRule annotation1
Binding sitei173Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei233SubstrateUniRule annotation1
Binding sitei277Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi78 – 79ATPUniRule annotation2
Nucleotide bindingi113 – 116ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processGlycolysisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:SRM_02332Imported
OrganismiSalinibacter ruber (strain M8)Imported
Taxonomic identifieri761659 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeSalinibacter
Proteomesi
  • UP000000933 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD5HB48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 308PFKInterPro annotationAdd BLAST302

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 138Substrate bindingUniRule annotation3
Regioni180 – 182Substrate bindingUniRule annotation3
Regioni283 – 286Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248869.
KOiK21071.
OMAiRDGWRGP.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiView protein in InterPro
IPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
IPR035966. PKF_sf.
PfamiView protein in Pfam
PF00365. PFK. 1 hit.
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiView protein in PROSITE
PS00433. PHOSPHOFRUCTOKINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

D5HB48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSDSLRVGV LTGGGDCPGI NAALRAVTKS LTLQHDAEVL GFLDGFRGVV
60 70 80 90 100
DRNVEPLHYR DVSGILTRGG TILGASNKAD PFNYLPRGGA DVSAQVMRTY
110 120 130 140 150
RTLDLDALVA IGGDGTMSIA HRLTDLGVDI VGVPKTIDND LVGTDRTVGF
160 170 180 190 200
DTAVSTATDA IDRIHTTAQS HHRVMIVETM GRYSGWIALH AGVASGTDVI
210 220 230 240 250
LLPEIEYEVE TVADVCRDRE RSGQRFTIVA VAEGARRKEG DYVVQERGDE
260 270 280 290 300
YTDSIRLGGI SRVLADQLRE HLDTSIRTTV LGHMQRGGSP TAYDRTLATT
310 320 330 340 350
LGTRAATLVA EGTTGVMVAV QNDAFTQVPL DEIAGKTRTV PLDDPLIASG
360 370
LDVGTSFGVA VSDLSEAASH SKPASPIQP
Length:379
Mass (Da):40,144
Last modified:June 15, 2010 - v1
Checksum:i61A6205E6B33A745
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP565814 Genomic DNA. Translation: CBH25253.1.
RefSeqiWP_011404839.1. NC_014032.1.

Genome annotation databases

EnsemblBacteriaiCBH25253; CBH25253; SRM_02332.
KEGGisrm:SRM_02332.
PATRICifig|761659.10.peg.2537.

Similar proteinsi

Entry informationi

Entry nameiD5HB48_SALRM
AccessioniPrimary (citable) accession number: D5HB48
Entry historyiIntegrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: October 25, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported