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D5H3A8

- D5H3A8_LACCS

UniProt

D5H3A8 - D5H3A8_LACCS

Protein

CCA-adding enzyme

Gene

cca

Organism
Lactobacillus crispatus (strain ST1)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotationSAAS annotation

    Catalytic activityi

    A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATP or CTP; via amide nitrogenUniRule annotation
    Binding sitei36 – 361ATP or CTPUniRule annotation
    Metal bindingi46 – 461MagnesiumUniRule annotation
    Metal bindingi48 – 481MagnesiumUniRule annotation
    Binding sitei117 – 1171ATP or CTPUniRule annotation
    Binding sitei160 – 1601ATP or CTPUniRule annotation
    Binding sitei163 – 1631ATP or CTPUniRule annotation
    Binding sitei166 – 1661ATP or CTPUniRule annotation
    Binding sitei169 – 1691ATP or CTPUniRule annotation

    GO - Molecular functioni

    1. ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-HAMAP
    3. CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
    4. CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
    5. magnesium ion binding Source: UniProtKB-HAMAP
    6. tRNA adenylyltransferase activity Source: UniProtKB-HAMAP
    7. tRNA binding Source: UniProtKB-HAMAP
    8. tRNA cytidylyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA repair Source: UniProtKB-KW
    2. tRNA 3'-terminal CCA addition Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

    Keywords - Biological processi

    RNA repairUniRule annotationSAAS annotation, tRNA processingUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciLCRI748671:GIX1-1110-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
    Alternative name(s):
    CCA tRNA nucleotidyltransferaseUniRule annotation
    tRNA CCA-pyrophosphorylaseUniRule annotation
    tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
    tRNA nucleotidyltransferaseUniRule annotation
    tRNA-NTUniRule annotation
    Gene namesi
    Name:ccaUniRule annotationImported
    Ordered Locus Names:LCRIS_01046Imported
    OrganismiLactobacillus crispatus (strain ST1)Imported
    Taxonomic identifieri748671 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000002371: Chromosome

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD5H3A8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000253346.
    KOiK00974.
    OMAiDITTSAY.

    Family and domain databases

    HAMAPiMF_01263. CCA_bact_type3.
    InterProiIPR023068. CCA-adding_enz_firmicutes.
    IPR002646. PolA_pol_head_dom.
    [Graphical view]
    PfamiPF01743. PolyA_pol. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D5H3A8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKIDNLPEI FTKAMPVLQI LENAGFEAYF VGGSVRDVLL HRHVHDVDIT    50
    TSAYPEEVKE LFDKSIDTGI KHGTVTVLYG GESYEITTFR TESGYQDFRR 100
    PDHVTFVQNL DEDLKRRDFT INALAMDTRG QIVDLFNGLD DLKNHVIRAV 150
    GDPEKRFHED ALRMMRAVRF MSQLEFKLES KTQQAIKDNH KLLKKISVER 200
    IREEFVKLGL GPHARQAFQV FLDTNLSEDV PDFGGKKDQL AIFPQLKFSP 250
    TMENSLWSLI ILLLKLPNEQ IHRFMRDWKN SNAMTEQVER IINLFDLLSS 300
    HAPSDYELFL AGEETLINTI DVAHIVGQPI SSEALVDRYM ALPIKKASEL 350
    AVDGRFLIDR GMRPGAKLGQ TLNKIRELVV SGEVENNQEA IESYLTTIE 399
    Length:399
    Mass (Da):45,574
    Last modified:June 15, 2010 - v1
    Checksum:i90CE4AF4AF161D75
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN692037 Genomic DNA. Translation: CBL50493.1.
    RefSeqiYP_003601518.1. NC_014106.1.

    Genome annotation databases

    EnsemblBacteriaiCBL50493; CBL50493; LCRIS_01046.
    GeneIDi9106371.
    KEGGilcr:LCRIS_01046.
    PATRICi37240911. VBILacCri149433_1022.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN692037 Genomic DNA. Translation: CBL50493.1 .
    RefSeqi YP_003601518.1. NC_014106.1.

    3D structure databases

    ProteinModelPortali D5H3A8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBL50493 ; CBL50493 ; LCRIS_01046 .
    GeneIDi 9106371.
    KEGGi lcr:LCRIS_01046.
    PATRICi 37240911. VBILacCri149433_1022.

    Phylogenomic databases

    HOGENOMi HOG000253346.
    KOi K00974.
    OMAi DITTSAY.

    Enzyme and pathway databases

    BioCyci LCRI748671:GIX1-1110-MONOMER.

    Family and domain databases

    HAMAPi MF_01263. CCA_bact_type3.
    InterProi IPR023068. CCA-adding_enz_firmicutes.
    IPR002646. PolA_pol_head_dom.
    [Graphical view ]
    Pfami PF01743. PolyA_pol. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ST1Imported.
    2. "Genome Sequence of Lactobacillus crispatus ST1."
      Ojala T., Kuparinen V., Koskinen J.P., Alatalo E., Holm L., Auvinen P., Edelman S., Westerlund-Wikstroem B., Korhonen T.K., Paulin L., Kankainen M.
      Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ST1.

    Entry informationi

    Entry nameiD5H3A8_LACCS
    AccessioniPrimary (citable) accession number: D5H3A8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 15, 2010
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3