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D5H3A8

- D5H3A8_LACCS

UniProt

D5H3A8 - D5H3A8_LACCS

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Protein

CCA-adding enzyme

Gene

cca

Organism
Lactobacillus crispatus (strain ST1)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotationSAAS annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotationSAAS annotation

Cofactori

Note: Magnesium.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATP or CTP; via amide nitrogenUniRule annotation
Binding sitei36 – 361ATP or CTPUniRule annotation
Metal bindingi46 – 461MagnesiumUniRule annotation
Metal bindingi48 – 481MagnesiumUniRule annotation
Binding sitei117 – 1171ATP or CTPUniRule annotation
Binding sitei160 – 1601ATP or CTPUniRule annotation
Binding sitei163 – 1631ATP or CTPUniRule annotation
Binding sitei166 – 1661ATP or CTPUniRule annotation
Binding sitei169 – 1691ATP or CTPUniRule annotation

GO - Molecular functioni

  1. ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-HAMAP
  3. CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
  4. CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
  5. magnesium ion binding Source: UniProtKB-HAMAP
  6. tRNA adenylyltransferase activity Source: UniProtKB-HAMAP
  7. tRNA binding Source: UniProtKB-HAMAP
  8. tRNA cytidylyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA repair Source: UniProtKB-KW
  2. tRNA 3'-terminal CCA addition Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

RNA repairUniRule annotationSAAS annotation, tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciLCRI748671:GIX1-1110-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotationImported
Ordered Locus Names:LCRIS_01046Imported
OrganismiLactobacillus crispatus (strain ST1)Imported
Taxonomic identifieri748671 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000002371: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD5H3A8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000253346.
KOiK00974.
OMAiDITTSAY.

Family and domain databases

HAMAPiMF_01263. CCA_bact_type3.
InterProiIPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5H3A8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKIDNLPEI FTKAMPVLQI LENAGFEAYF VGGSVRDVLL HRHVHDVDIT
60 70 80 90 100
TSAYPEEVKE LFDKSIDTGI KHGTVTVLYG GESYEITTFR TESGYQDFRR
110 120 130 140 150
PDHVTFVQNL DEDLKRRDFT INALAMDTRG QIVDLFNGLD DLKNHVIRAV
160 170 180 190 200
GDPEKRFHED ALRMMRAVRF MSQLEFKLES KTQQAIKDNH KLLKKISVER
210 220 230 240 250
IREEFVKLGL GPHARQAFQV FLDTNLSEDV PDFGGKKDQL AIFPQLKFSP
260 270 280 290 300
TMENSLWSLI ILLLKLPNEQ IHRFMRDWKN SNAMTEQVER IINLFDLLSS
310 320 330 340 350
HAPSDYELFL AGEETLINTI DVAHIVGQPI SSEALVDRYM ALPIKKASEL
360 370 380 390
AVDGRFLIDR GMRPGAKLGQ TLNKIRELVV SGEVENNQEA IESYLTTIE
Length:399
Mass (Da):45,574
Last modified:June 15, 2010 - v1
Checksum:i90CE4AF4AF161D75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN692037 Genomic DNA. Translation: CBL50493.1.
RefSeqiYP_003601518.1. NC_014106.1.

Genome annotation databases

EnsemblBacteriaiCBL50493; CBL50493; LCRIS_01046.
GeneIDi9106371.
KEGGilcr:LCRIS_01046.
PATRICi37240911. VBILacCri149433_1022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN692037 Genomic DNA. Translation: CBL50493.1 .
RefSeqi YP_003601518.1. NC_014106.1.

3D structure databases

ProteinModelPortali D5H3A8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CBL50493 ; CBL50493 ; LCRIS_01046 .
GeneIDi 9106371.
KEGGi lcr:LCRIS_01046.
PATRICi 37240911. VBILacCri149433_1022.

Phylogenomic databases

HOGENOMi HOG000253346.
KOi K00974.
OMAi DITTSAY.

Enzyme and pathway databases

BioCyci LCRI748671:GIX1-1110-MONOMER.

Family and domain databases

HAMAPi MF_01263. CCA_bact_type3.
InterProi IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
[Graphical view ]
Pfami PF01743. PolyA_pol. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ST1Imported.
  2. "Genome Sequence of Lactobacillus crispatus ST1."
    Ojala T., Kuparinen V., Koskinen J.P., Alatalo E., Holm L., Auvinen P., Edelman S., Westerlund-Wikstroem B., Korhonen T.K., Paulin L., Kankainen M.
    Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ST1.

Entry informationi

Entry nameiD5H3A8_LACCS
AccessioniPrimary (citable) accession number: D5H3A8
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: November 26, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3