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Protein

CCA-adding enzyme

Gene

cca

Organism
Lactobacillus crispatus (strain ST1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotationSAAS annotation

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATP or CTP; via amide nitrogenUniRule annotation1
Binding sitei36ATP or CTPUniRule annotation1
Metal bindingi46MagnesiumUniRule annotation1
Metal bindingi48MagnesiumUniRule annotation1
Binding sitei117ATP or CTPUniRule annotation1
Binding sitei160ATP or CTPUniRule annotation1
Binding sitei163ATP or CTPUniRule annotation1
Binding sitei166ATP or CTPUniRule annotation1
Binding sitei169ATP or CTPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferaseUniRule annotationSAAS annotation, RNA-bindingUniRule annotationSAAS annotation, Transferase
Biological processRNA repairUniRule annotationSAAS annotation, tRNA processingUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotationSAAS annotation (EC:2.7.7.72UniRule annotationSAAS annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotationImported
Ordered Locus Names:LCRIS_01046Imported
OrganismiLactobacillus crispatus (strain ST1)Imported
Taxonomic identifieri748671 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000002371 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi748671.LCRIS_01046.

Structurei

3D structure databases

ProteinModelPortaliD5H3A8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 148PolyA_polInterPro annotationAdd BLAST121
Domaini176 – 229PolyA_pol_RNAbdInterPro annotationAdd BLAST54
Domaini249 – 393tRNA_NucTran2_2InterPro annotationAdd BLAST145

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105D4J. Bacteria.
COG0617. LUCA.
HOGENOMiHOG000253346.
KOiK00974.
OMAiWKNSNAM.
OrthoDBiPOG091H022U.

Family and domain databases

CDDicd05398. NT_ClassII-CCAase. 1 hit.
HAMAPiMF_01263. CCA_bact_type3. 1 hit.
InterProiView protein in InterPro
IPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
PfamiView protein in Pfam
PF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.

Sequencei

Sequence statusi: Complete.

D5H3A8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKIDNLPEI FTKAMPVLQI LENAGFEAYF VGGSVRDVLL HRHVHDVDIT
60 70 80 90 100
TSAYPEEVKE LFDKSIDTGI KHGTVTVLYG GESYEITTFR TESGYQDFRR
110 120 130 140 150
PDHVTFVQNL DEDLKRRDFT INALAMDTRG QIVDLFNGLD DLKNHVIRAV
160 170 180 190 200
GDPEKRFHED ALRMMRAVRF MSQLEFKLES KTQQAIKDNH KLLKKISVER
210 220 230 240 250
IREEFVKLGL GPHARQAFQV FLDTNLSEDV PDFGGKKDQL AIFPQLKFSP
260 270 280 290 300
TMENSLWSLI ILLLKLPNEQ IHRFMRDWKN SNAMTEQVER IINLFDLLSS
310 320 330 340 350
HAPSDYELFL AGEETLINTI DVAHIVGQPI SSEALVDRYM ALPIKKASEL
360 370 380 390
AVDGRFLIDR GMRPGAKLGQ TLNKIRELVV SGEVENNQEA IESYLTTIE
Length:399
Mass (Da):45,574
Last modified:June 15, 2010 - v1
Checksum:i90CE4AF4AF161D75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN692037 Genomic DNA. Translation: CBL50493.1.
RefSeqiWP_005720213.1. NC_014106.1.

Genome annotation databases

EnsemblBacteriaiCBL50493; CBL50493; LCRIS_01046.
GeneIDi9106371.
KEGGilcr:LCRIS_01046.

Similar proteinsi

Entry informationi

Entry nameiD5H3A8_LACCS
AccessioniPrimary (citable) accession number: D5H3A8
Entry historyiIntegrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: September 27, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported