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Protein

CCA-adding enzyme

Gene

cca

Organism
Lactobacillus crispatus (strain ST1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotationSAAS annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATP or CTP; via amide nitrogenUniRule annotation
Binding sitei36 – 361ATP or CTPUniRule annotation
Metal bindingi46 – 461MagnesiumUniRule annotation
Metal bindingi48 – 481MagnesiumUniRule annotation
Binding sitei117 – 1171ATP or CTPUniRule annotation
Binding sitei160 – 1601ATP or CTPUniRule annotation
Binding sitei163 – 1631ATP or CTPUniRule annotation
Binding sitei166 – 1661ATP or CTPUniRule annotation
Binding sitei169 – 1691ATP or CTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

RNA repairUniRule annotationSAAS annotation, tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciLCRI748671:GIX1-1110-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotationImported
Ordered Locus Names:LCRIS_01046Imported
OrganismiLactobacillus crispatus (strain ST1)Imported
Taxonomic identifieri748671 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000002371 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi748671.LCRIS_01046.

Structurei

3D structure databases

ProteinModelPortaliD5H3A8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000253346.
KOiK00974.
OMAiDITTSAY.

Family and domain databases

HAMAPiMF_01263. CCA_bact_type3.
InterProiIPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D5H3A8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKIDNLPEI FTKAMPVLQI LENAGFEAYF VGGSVRDVLL HRHVHDVDIT
60 70 80 90 100
TSAYPEEVKE LFDKSIDTGI KHGTVTVLYG GESYEITTFR TESGYQDFRR
110 120 130 140 150
PDHVTFVQNL DEDLKRRDFT INALAMDTRG QIVDLFNGLD DLKNHVIRAV
160 170 180 190 200
GDPEKRFHED ALRMMRAVRF MSQLEFKLES KTQQAIKDNH KLLKKISVER
210 220 230 240 250
IREEFVKLGL GPHARQAFQV FLDTNLSEDV PDFGGKKDQL AIFPQLKFSP
260 270 280 290 300
TMENSLWSLI ILLLKLPNEQ IHRFMRDWKN SNAMTEQVER IINLFDLLSS
310 320 330 340 350
HAPSDYELFL AGEETLINTI DVAHIVGQPI SSEALVDRYM ALPIKKASEL
360 370 380 390
AVDGRFLIDR GMRPGAKLGQ TLNKIRELVV SGEVENNQEA IESYLTTIE
Length:399
Mass (Da):45,574
Last modified:June 15, 2010 - v1
Checksum:i90CE4AF4AF161D75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN692037 Genomic DNA. Translation: CBL50493.1.
RefSeqiWP_005720213.1. NC_014106.1.
YP_003601518.1. NC_014106.1.

Genome annotation databases

EnsemblBacteriaiCBL50493; CBL50493; LCRIS_01046.
GeneIDi9106371.
KEGGilcr:LCRIS_01046.
PATRICi37240911. VBILacCri149433_1022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN692037 Genomic DNA. Translation: CBL50493.1.
RefSeqiWP_005720213.1. NC_014106.1.
YP_003601518.1. NC_014106.1.

3D structure databases

ProteinModelPortaliD5H3A8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi748671.LCRIS_01046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBL50493; CBL50493; LCRIS_01046.
GeneIDi9106371.
KEGGilcr:LCRIS_01046.
PATRICi37240911. VBILacCri149433_1022.

Phylogenomic databases

HOGENOMiHOG000253346.
KOiK00974.
OMAiDITTSAY.

Enzyme and pathway databases

BioCyciLCRI748671:GIX1-1110-MONOMER.

Family and domain databases

HAMAPiMF_01263. CCA_bact_type3.
InterProiIPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ST1Imported.
  2. "Genome Sequence of Lactobacillus crispatus ST1."
    Ojala T., Kuparinen V., Koskinen J.P., Alatalo E., Holm L., Auvinen P., Edelman S., Westerlund-Wikstroem B., Korhonen T.K., Paulin L., Kankainen M.
    Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ST1.

Entry informationi

Entry nameiD5H3A8_LACCS
AccessioniPrimary (citable) accession number: D5H3A8
Entry historyi
Integrated into UniProtKB/TrEMBL: June 15, 2010
Last sequence update: June 15, 2010
Last modified: June 24, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.