D5H190 (D5H190_LACCS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A HAMAP-Rule MF_00171
EC=5.4.99.12 HAMAP-Rule MF_00171

Alternative name(s):
tRNA pseudouridine(38-40) synthase HAMAP-Rule MF_00171
tRNA pseudouridylate synthase I HAMAP-Rule MF_00171
tRNA-uridine isomerase I HAMAP-Rule MF_00171

Gene names

Name:	truA HAMAP-Rule MF_00171 EMBL CBL49775.1
Ordered Locus Names:	LCRIS_00328

Organism

Lactobacillus crispatus (strain ST1) [Complete proteome] [HAMAP] EMBL CBL49775.1

Taxonomic identifier

748671 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus crispatus

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171 SAAS SAAS020094
Catalytic activity	tRNA uridine = tRNA pseudouridine. RuleBase RU003792 tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171 SAAS SAAS020094
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family. HAMAP-Rule MF_00171 RuleBase RU003792

Ontologies

Keywords
Biological process	tRNA processing HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Molecular function	Isomerase HAMAP-Rule MF_00171 SAAS SAAS020094 RuleBase RU003792
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	54	1	Nucleophile By similarity HAMAP-Rule MF_00171
	Binding site	113	1	Substrate By similarity HAMAP-Rule MF_00171

Sequences

	Sequence		Length	Mass (Da)
	D5H190 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: BC64542698BBC271	FASTA	262	30,379
	10 20 30 40 50 60 MTTRYKMTMA YDGHLFHGFQ LQPDQRTVQG TIEDALKKMT KGQRVIVQGS GRTDAGVHAV 70 80 90 100 110 120 GQVIHFDYPG KTIPANRMIL AMNSMMPTDI IFDDCQIVDE NFHARYSIKG KWYRYRVSLD 130 140 150 160 170 180 HFVNPFKRFY TGHFPYPLDI EKMQVAAQDL LGKHDFTSFA ASGGQIENKV RTIYYVNITK 190 200 210 220 230 240 DEKENEVIFD FIGSGFLYNM VRIMVAALLE IGNDRRPIHD LQRVIAAKDR QEVRQTAHAS 250 260 GLYLYHVFYD EIPQKYRLDQ HL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of Lactobacillus crispatus ST1." Ojala T., Kuparinen V., Koskinen J.P., Alatalo E., Holm L., Auvinen P., Edelman S., Westerlund-Wikstrom B., Korhonen T.K., Paulin L., Kankainen M. J. Bacteriol. 192:3547-3548(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ST1.
[2]	"Genome Sequence of Lactobacillus crispatus ST1." Ojala T., Kuparinen V., Koskinen J.P., Alatalo E., Holm L., Auvinen P., Edelman S., Westerlund-Wikstroem B., Korhonen T.K., Paulin L., Kankainen M. Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ST1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN692037 Genomic DNA. Translation: CBL49775.1.
RefSeq	YP_003600800.1. NC_014106.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBL49775; CBL49775; LCRIS_00328.
GeneID	9107082.
KEGG	lcr:LCRIS_00328.
PATRIC	37239409. VBILacCri149433_0321.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000248673.
KO	K06173.
OMA	TPFRNVH.
Enzyme and pathway databases
BioCyc	LCRI748671:GIX1-342-MONOMER.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D5H190_LACCS

Accession

Primary (citable) accession number: D5H190

Entry history

Integrated into UniProtKB/TrEMBL:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)