D5GYB9 (D5GYB9_LACCS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
EC=2.1.2.9 HAMAP-Rule MF_00182

Gene names

Name:	fmt HAMAP-Rule MF_00182 EMBL CBL50778.1
Ordered Locus Names:	LCRIS_01331

Organism

Lactobacillus crispatus (strain ST1) [Complete proteome] [HAMAP] EMBL CBL50778.1

Taxonomic identifier

748671 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus crispatus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182 SAAS SAAS001555
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182 SAAS SAAS001555
Sequence similarities	Belongs to the fmt family. HAMAP-Rule MF_00182

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00182 SAAS SAAS001555
Molecular function	Methyltransferase HAMAP-Rule MF_00182 SAAS SAAS001555 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	110 – 113	4	Tetrahydrofolate (THF) binding By similarity HAMAP-Rule MF_00182

Sequences

	Sequence		Length	Mass (Da)
	D5GYB9 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: 45BAB52F1045D940	FASTA	314	34,335
	10 20 30 40 50 60 MTSIIFMGTP EFSVPVLEGL IDEGYEIKAV VTQPDKKVGR KQKITKSPAK ITAEKHDLPI 70 80 90 100 110 120 FQPVKLSGSD EMQKLIDMHA DLIVTAAYGQ FLPTKFLNSV KIAAVNVHGS LLPKYRGGAP 130 140 150 160 170 180 IQYSLINGDK ETGITIMEMV KKMDAGDIYA QEAIKIEPDD NAGTLFSKLS IVGRDLLLKT 190 200 210 220 230 240 LPAIIDGSIK KVPQDPEKVV FSPNITKEQE KLNVDMTAEE ANDMIRALNP DPGAYLMIEG 250 260 270 280 290 300 KRFKIWKAEI ASDSSSLNAG SVVANKGRFA ISFADNSVLN LLEVQPSGKK RMDIKSFLNG 310 QGSKFVPGEE IVDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of Lactobacillus crispatus ST1." Ojala T., Kuparinen V., Koskinen J.P., Alatalo E., Holm L., Auvinen P., Edelman S., Westerlund-Wikstrom B., Korhonen T.K., Paulin L., Kankainen M. J. Bacteriol. 192:3547-3548(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ST1.
[2]	"Genome Sequence of Lactobacillus crispatus ST1." Ojala T., Kuparinen V., Koskinen J.P., Alatalo E., Holm L., Auvinen P., Edelman S., Westerlund-Wikstroem B., Korhonen T.K., Paulin L., Kankainen M. Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ST1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN692037 Genomic DNA. Translation: CBL50778.1.
RefSeq	YP_003601803.1. NC_014106.1.
3D structure databases
ProteinModelPortal	D5GYB9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBL50778; CBL50778; LCRIS_01331.
GeneID	9107752.
KEGG	lcr:LCRIS_01331.
PATRIC	37241478. VBILacCri149433_1305.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
Enzyme and pathway databases
BioCyc	LCRI748671:GIX1-1395-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D5GYB9_LACCS

Accession

Primary (citable) accession number: D5GYB9

Entry history

Integrated into UniProtKB/TrEMBL:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)