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Protein

Probable dipeptidyl-aminopeptidase B

Gene

DAPB

Organism
Tuber melanosporum (strain Mel28) (Perigord black truffle)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.By similarity

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei746 – 7461Charge relay systemBy similarity
Active sitei823 – 8231Charge relay systemBy similarity
Active sitei856 – 8561Charge relay systemBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. serine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS09.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dipeptidyl-aminopeptidase B (EC:3.4.14.5)
Short name:
DPAP B
Gene namesi
Name:DAPB
ORF Names:GSTUM_00010539001
OrganismiTuber melanosporum (strain Mel28) (Perigord black truffle)
Taxonomic identifieri656061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaPezizomycetesPezizalesTuberaceaeTuber
ProteomesiUP000006911: Unassembled WGS sequence

Subcellular locationi

Vacuole membrane By similarity; Single-pass type II membrane protein By similarity
Note: Lysosome-like vacuoles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8888CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei89 – 10921Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini110 – 907798VacuolarSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 907907Probable dipeptidyl-aminopeptidase BPRO_0000412166Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi341 – 3411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi800 – 8001N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliD5GM60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9B family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiD5GM60.
KOiK01282.
OMAiDMSALQE.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

D5GM60-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYDQVPYRDT DEATPQIKND ASDSNRSSID TTSTTSLILE RLHREDPDDS
60 70 80 90 100
PGEYESSGPS QRGKPDEDDD LEIGRAARLK PMERKVRRAM YLLAFLMIGG
110 120 130 140 150
WFLALAVYVS REHFGTPDTA HDPSATATRK AGKKITLNQV MRGAWRSKTH
160 170 180 190 200
GIQWINGPHG DQDGLLLTQN SFGDGNFLEV QDVKNDSNTI VLIKDGALQG
210 220 230 240 250
SGQPVSAIKG WPSSDLKKVL VASDHEKRWR HSYNARYWIY DVEKATTEPL
260 270 280 290 300
VPSEPEARLS LATWSPKGDA IAFVKDNNVF IRQLGLDLTS EYYSVTQVTK
310 320 330 340 350
DGGPDLFYGI PDWVYEEEVF SGNSALWWSQ DGEFLAFLRT NETEVPEYPI
360 370 380 390 400
QYFVSRPSGN NPPNGLENYP ELEFIKYPKA GAPNPVVHLR FYDLKKKEDF
410 420 430 440 450
AVTVENDFPD DDRLITEVVW SDGKYLLVRE TNRESDVLRM VLIDVSARSG
460 470 480 490 500
KVVREVDISA IDGGWFEVSK NTRYIPADPA SGRPYEGYID TVIHEGYDHL
510 520 530 540 550
GYFTPLDNKD PILLTKGQWE VVDAPSAVDL KNGIVYFVAT EKSPIERHVY
560 570 580 590 600
SVKLDGSNFR PVTSTSEDGR YDVSFSKLSG YALLTYEGPG IPWQKVVGTP
610 620 630 640 650
SGDQSFVKDI EKNQGLATLA AKHELPTFHY STVNIDGFDL HVVERRPPHF
660 670 680 690 700
NKKRKYPVLF QVYGGPGSQQ VSKSFSIDFQ AYIAAGLEYI VVTVDGRGTG
710 720 730 740 750
FIGRKARVAV RGNLGYWEAH DQIETAKIWG KKGYVDKKRI AIWGWSYGGF
760 770 780 790 800
MTLKTLEQDA GRTFSYGMAV APVTDWRFYD SIYTERYMHT PQHNQEGYRN
810 820 830 840 850
ATISDTQALS KSVRFLLMHG VADDNVHMQN SLALLDKLDL ASVENYDVHV
860 870 880 890 900
FPDSDHSIYF HNANRMVYDR LEQWLIRAFN GEFLKLDGLK PIREISEPLR

KRNRELV
Length:907
Mass (Da):102,621
Last modified:June 15, 2010 - v1
Checksum:i137143AE5B685B7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN430352 Genomic DNA. Translation: CAZ85603.1.
RefSeqiXP_002841412.1. XM_002841366.1.
UniGeneiTme.1126.

Genome annotation databases

EnsemblFungiiCAZ85603; CAZ85603; GSTUM_00010539001.
GeneIDi9187387.
KEGGitml:GSTUM_00010539001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN430352 Genomic DNA. Translation: CAZ85603.1.
RefSeqiXP_002841412.1. XM_002841366.1.
UniGeneiTme.1126.

3D structure databases

ProteinModelPortaliD5GM60.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS09.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAZ85603; CAZ85603; GSTUM_00010539001.
GeneIDi9187387.
KEGGitml:GSTUM_00010539001.

Phylogenomic databases

InParanoidiD5GM60.
KOiK01282.
OMAiDMSALQE.
OrthoDBiEOG72VHFG.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mel28.

Entry informationi

Entry nameiDAPB_TUBMM
AccessioniPrimary (citable) accession number: D5GM60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: June 15, 2010
Last modified: January 7, 2015
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.